[ Objective] To investigate the optimal culture system for in vitro fertilization （IVF） of oocyte in bovine. [ Method] The IVF of mature bovine oocytes was conducted and the fertilized eggs were cultured in vitro wi...[ Objective] To investigate the optimal culture system for in vitro fertilization （IVF） of oocyte in bovine. [ Method] The IVF of mature bovine oocytes was conducted and the fertilized eggs were cultured in vitro with different culture systems. In the traditional culture system, the TALP medium and BO medium were used as fertilization medium, respectively; and M-199 medium supplemented with 5% FCS was used as fertilized egg culture medium. In the transitional culture system, the TALP medium and BO medium were used as fertilization medium; 60% TALP and 40% M-199 supplemented with 5% FCS and 60% BO and 40% M-199 supplemented with 5% FCS were used as fertilized egg culture medium, respectively. The effects of different culture systems on fertilization rate and cleavage rate were observed. E Result] In the transitional culture system （with TALP medium as fertilization medium and with 60% TALP and 40% M-199 supplemented with 5% FCS as fertilized egg culture medium）, the fertilization rate and cleavage rate were increased to 77.8% and 55.6%, respectively. [ Conclusion] The transitional culture system （with TALP medium as fertilization medium and with 60% TALP and 40% M-199 supplemented with 5% FCS as fertilized egg culture medium） is the optimal culture system for IVF of bovine oocyte.展开更多
基金supported by the Scientific Research & Development Fund of Tianjin Agricultural University
文摘[ Objective] To investigate the optimal culture system for in vitro fertilization （IVF） of oocyte in bovine. [ Method] The IVF of mature bovine oocytes was conducted and the fertilized eggs were cultured in vitro with different culture systems. In the traditional culture system, the TALP medium and BO medium were used as fertilization medium, respectively; and M-199 medium supplemented with 5% FCS was used as fertilized egg culture medium. In the transitional culture system, the TALP medium and BO medium were used as fertilization medium; 60% TALP and 40% M-199 supplemented with 5% FCS and 60% BO and 40% M-199 supplemented with 5% FCS were used as fertilized egg culture medium, respectively. The effects of different culture systems on fertilization rate and cleavage rate were observed. E Result] In the transitional culture system （with TALP medium as fertilization medium and with 60% TALP and 40% M-199 supplemented with 5% FCS as fertilized egg culture medium）, the fertilization rate and cleavage rate were increased to 77.8% and 55.6%, respectively. [ Conclusion] The transitional culture system （with TALP medium as fertilization medium and with 60% TALP and 40% M-199 supplemented with 5% FCS as fertilized egg culture medium） is the optimal culture system for IVF of bovine oocyte.
