Fourier-domain-compressed optical time-stretch quantitative phase imaging flow cytometry

Optical time-stretch(OTS)imaging flow cytometry offers a promising solution for high-throughput and highprecision cell analysis due to its capabilities of high-speed,high-quality,and continuous imaging.Compressed sensing(CS)makes it practically applicable by significantly reducing the data volume while maintaining its highspeed and high-quality imaging properties.To enrich the information of the images acquired with CS-equipped OTS imaging flow cytometry,in this work we propose and experimentally demonstrate Fourier-domaincompressed OTS quantitative phase imaging flow cytometry.It is capable of acquiring intensity and quantitative phase images of cells simultaneously from the compressed data.To evaluate the performance of our method,static microparticles and a corn root cross section are experimentally measured under various compression ratios.Furthermore,to show how our method can be applied in practice,we utilize it in the drug response analysis of breast cancer cells.Experimental results show that our method can acquire high-quality intensity and quantitative phase images of flowing cells at a flowing speed of 1 m/s and a compression ratio of 30%.Combined with machine-learning-based image analysis,it can distinguish drug-treated and drug-untreated cells with an accuracy of over 95%.We believe our method can facilitate cell analysis in both scientific research and clinical settings where both high-throughput and high-content cell analysis is required.

Ultrafast imaging for uncovering laser–material interaction dynamics

The physical mechanism of the dynamics in laser–material interaction has been an important research area.In addition to theoretical analysis,direct imaging‐based observation of ultrafast dynamic processes is an important approach to understand many fundamental issues in laser–material interaction such as inertial confinement fusion(ICF),laser accelerator construction,and advanced laser production.In this review,the principles and applications of three types of commonly used ultrafast imaging methods are introduced,including the pump–probe,X‐ray diagnosis,and single‐shot optical burst imaging.We focus on the technical features such as the spatial and temporal resolution for each technique,and present several conventional applications.