One antibacterial activity fraction from an immunized dipteran insect, Bactrocera dorsalis, was isolated and purified by prepurification, ion-exchange chromatography, gel filtration chromatography and reverse-phase hi...One antibacterial activity fraction from an immunized dipteran insect, Bactrocera dorsalis, was isolated and purified by prepurification, ion-exchange chromatography, gel filtration chromatography and reverse-phase high performance liquid chromatography （HPLC）. The final purified fraction was checked on the Smart system HPLC and was judged as a pure fraction. The results of physical and biological analysis revealed that this fraction is heat stable and showed strong activities against Gram-positive bacterial growth. It possesses antibicrobial peptide properties and is worth further investigation.展开更多
Male-specific wing spots are usually associated with wing displays in the courtship behavior of Drosophila and may play important roles in sexual selection.Two closely related species,D.nepalensis and D.trilutea,diffe...Male-specific wing spots are usually associated with wing displays in the courtship behavior of Drosophila and may play important roles in sexual selection.Two closely related species,D.nepalensis and D.trilutea,differ in wing spots and scissoring behavior.Here,we compare male morphological characters,pigmentation intensity of male wing spots,wing-scissoring behavior,courtship songs,and reproductive isolation between 2 species.F1 fertile females and sterile males result from the cross between females of D.nepalensis and males of D.trilutea.The pigmentation of wing spots is significantly weaker in D.trilutea than in D.nepalensis and the F1 hybrid.Males scissor both wings in front of the female during courtship,with a posture spreading wings more widely,and at a faster frequency in D.nepalensis than in D.trilutea and the F1s.Males of D.trilutea vibrate wings to produce 2 types(A and B)of pulse songs,whereas D.nepalensis and the F1s sing only type B songs.The incidence of wing vibration and scissoring during courtship suggests that wing vibration is essential but scissoring is a facultative courtship element for successful mating in both species.The association between the darker wing spots with more elaborate scissoring might be the consequence of correlated evolution of these traits in D.nepalensis;however,D.trilutea retains wing scissoring during courtship despite having weaker pigmentation of wing spots.The genetic architecture of 2 traits differs in the F1s,consistent with maternal or sex-linked effects for spots but nonadditive effects for scissoring.展开更多
基金Acknowledgments We thank Prof. X.-F. Pang, late Academician of Chinese Academy of Science, for his support in getting this project started. We also thank Prof. Yamakawa （National of Institute of Sericultural and Entomological Science, Japan） for analyzing the purity of the final purified fraction. We thank Dr H. Ma for his suggestions on this project and reading our manuscript. This work was supported partly by the Natural Science Foundation of Guangdong Province （032256）, the National Basic Research Program of China under grant 2005CB121000 and the National Natural Science Foundation （30370716）.
文摘One antibacterial activity fraction from an immunized dipteran insect, Bactrocera dorsalis, was isolated and purified by prepurification, ion-exchange chromatography, gel filtration chromatography and reverse-phase high performance liquid chromatography （HPLC）. The final purified fraction was checked on the Smart system HPLC and was judged as a pure fraction. The results of physical and biological analysis revealed that this fraction is heat stable and showed strong activities against Gram-positive bacterial growth. It possesses antibicrobial peptide properties and is worth further investigation.
基金funded by the National Scientifie Foundation of China(31372187)funded by the NERC,UK(grants NE/E015255/1 and NE/020818/1).
文摘Male-specific wing spots are usually associated with wing displays in the courtship behavior of Drosophila and may play important roles in sexual selection.Two closely related species,D.nepalensis and D.trilutea,differ in wing spots and scissoring behavior.Here,we compare male morphological characters,pigmentation intensity of male wing spots,wing-scissoring behavior,courtship songs,and reproductive isolation between 2 species.F1 fertile females and sterile males result from the cross between females of D.nepalensis and males of D.trilutea.The pigmentation of wing spots is significantly weaker in D.trilutea than in D.nepalensis and the F1 hybrid.Males scissor both wings in front of the female during courtship,with a posture spreading wings more widely,and at a faster frequency in D.nepalensis than in D.trilutea and the F1s.Males of D.trilutea vibrate wings to produce 2 types(A and B)of pulse songs,whereas D.nepalensis and the F1s sing only type B songs.The incidence of wing vibration and scissoring during courtship suggests that wing vibration is essential but scissoring is a facultative courtship element for successful mating in both species.The association between the darker wing spots with more elaborate scissoring might be the consequence of correlated evolution of these traits in D.nepalensis;however,D.trilutea retains wing scissoring during courtship despite having weaker pigmentation of wing spots.The genetic architecture of 2 traits differs in the F1s,consistent with maternal or sex-linked effects for spots but nonadditive effects for scissoring.
