Dihydroartemisinin attenuates ischemia/reperfusion-induced renal tubular senescence by activating autophagy

Acute kidney injury(AKI)is an important factor for the occurrence and development of CKD.The protective effect of dihydroartemisinin on AKI and and reported mechanism have not been reported.In this study,we used two animal models including ischemia-reperfusion and UUO,as well as a high-glucose-stimulated HK-2 cell model,to evaluate the protective effect of dihydroartemisinin on premature senescence of renal tubular epithelial cells in vitro and in vivo.We demonstrated that dihydroartemisinin improved renal aging and renal injury by activating autophagy.In addition,we found that co-treatment with chloroquine,an autophagy inhibitor,abolished the anti-renal aging effect of dihydroartemisinin in vitro.These findings suggested that activation of autophagy/elimination of senescent cell might be a useful strategy to prevent AKI/UUO induced renal tubular senescence and fibrosis.

两栖类心脏起搏点观察实验中剪断法与传统结扎法的比较

斯氏结扎法是两栖类心脏起搏点观察的经典方法,但成功率较低.将斯氏结扎法改为剪断法,并从成功率、心率恢复时间及恢复后心率等方面对2种方法进行比较.结果表明,斯氏第一扎成功率为47.6%,心率恢复时间为21.0 min,而剪断法的窦一房分割成功率为83.3%,心率恢复时间仅3.8 min.此外,在孵育液中添加肝素和通氧均可显著延长心搏维持时间.剪断法具有操作简单、成功率高、恢复时间短等诸多优点,可代替结扎法用于起搏点观察实验.