Treatment of malignant brain tumors continues to challenge scientists and clinicians alike.Location of these tumors within the central nervous system(CNS),which is considered a'privileged" organ,can prevent t...Treatment of malignant brain tumors continues to challenge scientists and clinicians alike.Location of these tumors within the central nervous system(CNS),which is considered a'privileged" organ,can prevent the penetration of chemotherapeutic agents through the blood-brain barrier(BBB).To overcome this limitation,nanoparticles are taken up and transported bymacrophage and then delivered directly into the CNs.In this study,we used macrophage touptake the folate-targeted bifunctional miclles loaded with near-infrared(NIR)dye ICG-Der-01and investigate the dynamic bio-distributions of macrophage after intravenous injection intotumor-bearing mice.In vitro cellular experiments by confocal microscopy indicated that theuptake of micelles in macrophage was greatly enhanced due to the folate receptor overexpression.Dynamic bio-distributions of macrophage showed a rapid clearing rate through the liver int estine path way.In conclusion,macrophage could potentially be used as nanoparticle drug carriers andrequire further investigation.展开更多
A novel near-infrared light responsive microcapsule sy stem,gold nanorod-covered DoX-loadedhollow CaCO;microcapsule(AuNR-HM-DOX)is developed for cancer therapy.The hollowCaCOs microcapsules were prepared based on the ...A novel near-infrared light responsive microcapsule sy stem,gold nanorod-covered DoX-loadedhollow CaCO;microcapsule(AuNR-HM-DOX)is developed for cancer therapy.The hollowCaCOs microcapsules were prepared based on the selfassembly between chitosan and sodiumalgimate on CaCOs Particles via layer-by-layer technique,and then coveredl with gold nanorods toobtain the microcapsule systemn.Upon near-infrared(NIR)irradiation,microcapsule with goldnanorods can convert the absorbed NIR light into heat.Meanwhile,doxorubicin(DOX),a che.motherapy drug,is loaded into the microcapsule system via electrostatic adsorption for combinedphotothermal therapy and chemotherapy,Properties of AuNR-HM-DOX including grain dia-meter,optical spectra were characterized'Confocal fluorescence imaging was performed to observet he morphology of the capsules and existence of D OX in the core,confrming t he successful loading of DOX.The release of DOX from the capsules under continuous NIR rradiation was investigatedto evaluate the temperat ure responsiveness of AuNR-HMDOX.Results indicate that AuNR-HM.DOX microcapsules possess uniform particle size and high light responsiveness.The combination of chemical and physical therapy of AuNR-HMDOX features great potential as an adjuvanttherapeutic alternative material for combined cancer therapy.展开更多
基金grateful to Natural Science Foundation Committee of China(NSFC 81220108012,81171395,81071194,81000666,30970776,30672015,30800257and 31050110123)the Ministry of Science and Technology(2009ZX09310-004)the Priority Academic Program Development of Jiangsu Higher Education Institutions for their financial support.
文摘Treatment of malignant brain tumors continues to challenge scientists and clinicians alike.Location of these tumors within the central nervous system(CNS),which is considered a'privileged" organ,can prevent the penetration of chemotherapeutic agents through the blood-brain barrier(BBB).To overcome this limitation,nanoparticles are taken up and transported bymacrophage and then delivered directly into the CNs.In this study,we used macrophage touptake the folate-targeted bifunctional miclles loaded with near-infrared(NIR)dye ICG-Der-01and investigate the dynamic bio-distributions of macrophage after intravenous injection intotumor-bearing mice.In vitro cellular experiments by confocal microscopy indicated that theuptake of micelles in macrophage was greatly enhanced due to the folate receptor overexpression.Dynamic bio-distributions of macrophage showed a rapid clearing rate through the liver int estine path way.In conclusion,macrophage could potentially be used as nanoparticle drug carriers andrequire further investigation.
基金grateful to Natural Science Foun-dation Committee of China(NSFC 81220108012,81171395,81071194,81000666,30970776,30672015,30800257and 31050110123)the Ministry,of Scienceand Technology(2009ZX09310-004)the Pri-ority Academic Program Development of Jiangsu Higher Education Institutions for their financial support.
文摘A novel near-infrared light responsive microcapsule sy stem,gold nanorod-covered DoX-loadedhollow CaCO;microcapsule(AuNR-HM-DOX)is developed for cancer therapy.The hollowCaCOs microcapsules were prepared based on the selfassembly between chitosan and sodiumalgimate on CaCOs Particles via layer-by-layer technique,and then coveredl with gold nanorods toobtain the microcapsule systemn.Upon near-infrared(NIR)irradiation,microcapsule with goldnanorods can convert the absorbed NIR light into heat.Meanwhile,doxorubicin(DOX),a che.motherapy drug,is loaded into the microcapsule system via electrostatic adsorption for combinedphotothermal therapy and chemotherapy,Properties of AuNR-HM-DOX including grain dia-meter,optical spectra were characterized'Confocal fluorescence imaging was performed to observet he morphology of the capsules and existence of D OX in the core,confrming t he successful loading of DOX.The release of DOX from the capsules under continuous NIR rradiation was investigatedto evaluate the temperat ure responsiveness of AuNR-HMDOX.Results indicate that AuNR-HM.DOX microcapsules possess uniform particle size and high light responsiveness.The combination of chemical and physical therapy of AuNR-HMDOX features great potential as an adjuvanttherapeutic alternative material for combined cancer therapy.
