Characterizing how chemical compounds binding to serum albumin is essential in evaluating drug candidates and is the focus of this study. A surface plasmon resonance biosenser developed in this laboratory was used to ...Characterizing how chemical compounds binding to serum albumin is essential in evaluating drug candidates and is the focus of this study. A surface plasmon resonance biosenser developed in this laboratory was used to determine the binding constants of antibiotics with serum albumin. The binding constants of five antibiotics（asithromycin, spectinomycin, gentamycin, metacycline and kanamycin） with serum albumins were obtained.展开更多
We tried to develop an efficient strategy to isolate geniposide from Gardenia fruit and use it to treat chronic pain. Eight extraction solvents and three extraction methods were respectively compared with each other f...We tried to develop an efficient strategy to isolate geniposide from Gardenia fruit and use it to treat chronic pain. Eight extraction solvents and three extraction methods were respectively compared with each other for the efficiency of geniposide extraction from Gardenia fruit. Extraction with 80% ethanol under reflux was found to give the highest yield of geniposide. Orthogonal experiment was conducted to optimize the reflux conditions and the optimal conditions are a mass（of Gardeniajasminoides Ellis powder） to volume（of solvent, 80% ethanol） ratio of 1：7 and reflux twice for 60 rain each. The geniposide obtained by the reflux method was tested on the rats with chronic constriction injury（CCI） for pain relief. The results show that geniposide can enhance pain threshold and relieve pain via repressing tumor necrosis factor-a（TNF-a） expression in either ipsilateral or contralateral dorsal root gan- glion（DRG） cells. These results suggest that geniposide treatement may be an effective clinical method to relieve pain based on the mechanism of molecular biology at DRG due to nerve injury.展开更多
文摘Characterizing how chemical compounds binding to serum albumin is essential in evaluating drug candidates and is the focus of this study. A surface plasmon resonance biosenser developed in this laboratory was used to determine the binding constants of antibiotics with serum albumin. The binding constants of five antibiotics（asithromycin, spectinomycin, gentamycin, metacycline and kanamycin） with serum albumins were obtained.
基金Supported by the Natural Science Foundation of Jilin Province, China（No.20125059）.
文摘We tried to develop an efficient strategy to isolate geniposide from Gardenia fruit and use it to treat chronic pain. Eight extraction solvents and three extraction methods were respectively compared with each other for the efficiency of geniposide extraction from Gardenia fruit. Extraction with 80% ethanol under reflux was found to give the highest yield of geniposide. Orthogonal experiment was conducted to optimize the reflux conditions and the optimal conditions are a mass（of Gardeniajasminoides Ellis powder） to volume（of solvent, 80% ethanol） ratio of 1：7 and reflux twice for 60 rain each. The geniposide obtained by the reflux method was tested on the rats with chronic constriction injury（CCI） for pain relief. The results show that geniposide can enhance pain threshold and relieve pain via repressing tumor necrosis factor-a（TNF-a） expression in either ipsilateral or contralateral dorsal root gan- glion（DRG） cells. These results suggest that geniposide treatement may be an effective clinical method to relieve pain based on the mechanism of molecular biology at DRG due to nerve injury.
