在基于SDN架构的混合卫星网络上讨论了服务功能链(Service Function Chain,SFC)的可靠性部署问题,首先对SFC可靠性保护的问题进行描述,建立了底层网络与SFC请求模型,然后建立了网络服务功能的可靠性需求模型与低轨卫星链路的可靠性需求...在基于SDN架构的混合卫星网络上讨论了服务功能链(Service Function Chain,SFC)的可靠性部署问题,首先对SFC可靠性保护的问题进行描述,建立了底层网络与SFC请求模型,然后建立了网络服务功能的可靠性需求模型与低轨卫星链路的可靠性需求模型,明确了优化目标与约束条件。接着提出基于可靠性的卫星服务功能链保护方法,包括基于深度强化学习的可靠性保护算法和基于低轨卫星节点与链路可靠性备份算法。实验表明,提出的基于可靠性的卫星服务功能链保护方法能在SDN架构的混合卫星网络上提高SFC请求接受率,减少平均时延,在不同的SFC可靠性需求的条件下也保持较高的请求接受率。展开更多
Neurogenesis is an important progress wherein the neural stem cells(NSCs) differentiate into functional neurons under conductive conditions. Neurogenesis occurs continuously in different areas of the central neural sy...Neurogenesis is an important progress wherein the neural stem cells(NSCs) differentiate into functional neurons under conductive conditions. Neurogenesis occurs continuously in different areas of the central neural system in adult teleosts compared with adult mammals. Therefore, NSC cell lines must be established to offer a valuable in vitro system for studies on neurogenesis and other related functions. In this study, four cell lines designated as PoB1, PoB2, PoBf and PoBh were established from the brain of the Japanese flounder Paralichthys olivaceus. The cell lines were sub-cultured over 150 times and still grew well in DMEM/F12 medium at 24℃. PoB1, PoB2, PoBf and PoBh were identified as neural stem/progenitor cell lines on the basis of the mRNA expression of nestin and/or aldh111 or slc6 a4 and the formation of neurospheres. The cells transfected with the pEGFP-C1 plasmid showed fluorescent signals with distinct reagent dependencies. The established cell lines from the brain of P. olivaceus offer a valuable system in vitro for the study of neurogenesis, fish neural regulation and endocrinology-related functions.展开更多
文摘在基于SDN架构的混合卫星网络上讨论了服务功能链(Service Function Chain,SFC)的可靠性部署问题,首先对SFC可靠性保护的问题进行描述,建立了底层网络与SFC请求模型,然后建立了网络服务功能的可靠性需求模型与低轨卫星链路的可靠性需求模型,明确了优化目标与约束条件。接着提出基于可靠性的卫星服务功能链保护方法,包括基于深度强化学习的可靠性保护算法和基于低轨卫星节点与链路可靠性备份算法。实验表明,提出的基于可靠性的卫星服务功能链保护方法能在SDN架构的混合卫星网络上提高SFC请求接受率,减少平均时延,在不同的SFC可靠性需求的条件下也保持较高的请求接受率。
基金supported by the National High-Tech Research and Development Program of China (Nos. 2012AA10A402,2012AA10A408)。
文摘Neurogenesis is an important progress wherein the neural stem cells(NSCs) differentiate into functional neurons under conductive conditions. Neurogenesis occurs continuously in different areas of the central neural system in adult teleosts compared with adult mammals. Therefore, NSC cell lines must be established to offer a valuable in vitro system for studies on neurogenesis and other related functions. In this study, four cell lines designated as PoB1, PoB2, PoBf and PoBh were established from the brain of the Japanese flounder Paralichthys olivaceus. The cell lines were sub-cultured over 150 times and still grew well in DMEM/F12 medium at 24℃. PoB1, PoB2, PoBf and PoBh were identified as neural stem/progenitor cell lines on the basis of the mRNA expression of nestin and/or aldh111 or slc6 a4 and the formation of neurospheres. The cells transfected with the pEGFP-C1 plasmid showed fluorescent signals with distinct reagent dependencies. The established cell lines from the brain of P. olivaceus offer a valuable system in vitro for the study of neurogenesis, fish neural regulation and endocrinology-related functions.
