Aristolochic acid （AA） is a known nephrotoxin and potential carcinogen, which can form covalent DNA adducts after metabolic activation in vivo and in vitro. A simple method for preparation and characterization of ar...Aristolochic acid （AA） is a known nephrotoxin and potential carcinogen, which can form covalent DNA adducts after metabolic activation in vivo and in vitro. A simple method for preparation and characterization of aristolochic acid-DNA adducts was developed. Four AA-adducts were synthesized by a direct reaction of AAI/AAII with 2′-deoxynucleosides. The reaction mixture was first cleaned-up and pre-concentrated using solid phase extraction （SPE）, and further purified by a reversed-phase high performance liquid chromatography （HPLC）. By the application of developed SPE procedure, matrices and byproducts in reaction mixture could be greatly reduced and adducts of high purity （more than 94% as indicated by HPLC） were obtained. The purified AA-DNA adducts were identified and characterized with liquid-electrospray ionization-quadrupole-time of flight-mass spectrometry （LC-ESI-Q-TOF-MS/MS） and LC-Diode array detector-fluorescence （LC-DAD-FL） analysis. This work provides a robust tool for possible large-scale preparation of AA-DNA adduct standards, which can promote the further studies on carcinogenic and mutagenic mechanism of aristolochic acids.展开更多
A preliminary survey of estrogenic activity of the contaminant in part of waters (Ziya River and the estu- ary of Haihe River) in Haihe River, Tianjin has been per- formed with the plasma vitellogenin (Vtg) in feral f...A preliminary survey of estrogenic activity of the contaminant in part of waters (Ziya River and the estu- ary of Haihe River) in Haihe River, Tianjin has been per- formed with the plasma vitellogenin (Vtg) in feral fish as a biomarker for estrogenic activity. The concentrations of bisphenol A (BPA), 4-tert-octylphenol (OP) and 4-nonyl- phenol (NP) in surface water were also determined. The presence of Vtg in male fish plasma as well as that in female can be detected at different sites and different seasons. The results indicate that the water in the sampling sites was con- taminated by some estrogenic compounds. Although BPA, OP and NP can be detected in all of the water samples, their concentrations were much lower than the effective concen- trations for those chemicals to induce Vtg production in male fish.展开更多
基金supported by the National Basic Research Program (973) of China (No. 2007CB407305,2008CB417201)the National High Technology Research and Development Program (863) of China (No.2007AA06A407)the National Natural Science Foundation of China (No. 20737003, 20621703, 20805057)
文摘Aristolochic acid （AA） is a known nephrotoxin and potential carcinogen, which can form covalent DNA adducts after metabolic activation in vivo and in vitro. A simple method for preparation and characterization of aristolochic acid-DNA adducts was developed. Four AA-adducts were synthesized by a direct reaction of AAI/AAII with 2′-deoxynucleosides. The reaction mixture was first cleaned-up and pre-concentrated using solid phase extraction （SPE）, and further purified by a reversed-phase high performance liquid chromatography （HPLC）. By the application of developed SPE procedure, matrices and byproducts in reaction mixture could be greatly reduced and adducts of high purity （more than 94% as indicated by HPLC） were obtained. The purified AA-DNA adducts were identified and characterized with liquid-electrospray ionization-quadrupole-time of flight-mass spectrometry （LC-ESI-Q-TOF-MS/MS） and LC-Diode array detector-fluorescence （LC-DAD-FL） analysis. This work provides a robust tool for possible large-scale preparation of AA-DNA adduct standards, which can promote the further studies on carcinogenic and mutagenic mechanism of aristolochic acids.
基金This work was supported by the High Tech Re-search and Development Progran of China (“863” Plan) (Grant No.2003AA640610) the National Natural Science Foundation of China (Grant No. 20137010) and the Chinese Academy of Sciences (Grant No.KZCX2-4 14).
文摘A preliminary survey of estrogenic activity of the contaminant in part of waters (Ziya River and the estu- ary of Haihe River) in Haihe River, Tianjin has been per- formed with the plasma vitellogenin (Vtg) in feral fish as a biomarker for estrogenic activity. The concentrations of bisphenol A (BPA), 4-tert-octylphenol (OP) and 4-nonyl- phenol (NP) in surface water were also determined. The presence of Vtg in male fish plasma as well as that in female can be detected at different sites and different seasons. The results indicate that the water in the sampling sites was con- taminated by some estrogenic compounds. Although BPA, OP and NP can be detected in all of the water samples, their concentrations were much lower than the effective concen- trations for those chemicals to induce Vtg production in male fish.
