Low stature in wheat is closely associated with lodging resistance,and this impacts harvest index and grain yield.The discovery of novel dwarfing or semi-dwarfing genes can have great significance for dwarf wheat bree...Low stature in wheat is closely associated with lodging resistance,and this impacts harvest index and grain yield.The discovery of novel dwarfing or semi-dwarfing genes can have great significance for dwarf wheat breeding.In this study,we identified an EMS induced dwarf wheat mutant JE0124 from the elite cultivar Jing411.JE0124 possesses increased stem strength and a 33%reduction in plant height compared with wild type.Giberelic acid(GA)treatment analysis suggested that JE0124 was GA-sensitive.Analysis of the frequency distribution of plant height in four F,populations derived from crosses between JE0124 and the relatively taller varieties Nongda 5181 and WT indicated that the dwarfism phenotype was quantitatively inherited.We used two F,populations and 312 individuals from the reciprocal cross of Nongda 5181 and JE0124 to map the quantitative trait locus(QTL)for reduced height to a 0.85-cM interval on chromosome 2DL.The mapping was done by using a combination of 660K SNP array-based bulked segregant analysis(BSA)and genetic linkage analysis,with logarithm of odds(LOD)scores of 5.34 and 5.78,respectively.Additionally,this QTL accounted for 8.27-8.52%of the variation in the phenotype.The dwarf mutant JE0124 and the newly discovered dwarfing gene on chromosome 2DL in this study will enrich genetic resources for dwarf wheat breeding.展开更多
Maize rough dwarf disease(MRDD) is a viral disease caused by brown planthopper infestation, and leads to great yield loss, especially in China. Comparative proteomics was performed using maize inbred line Zheng 58 and...Maize rough dwarf disease(MRDD) is a viral disease caused by brown planthopper infestation, and leads to great yield loss, especially in China. Comparative proteomics was performed using maize inbred line Zheng 58 and LN 287. MRDD pathogen was detected as rice black-streaked dwarf virus(RBSDV) by quantitative real time PCR(q RT-PCR) in Shandong Province, China. The modified trichloroacetic acid(TCA)/acetone method was used for soluble protein extraction from leaves. Two-dimensional electrophoresis(2-DE) analysis was performed on 24-cm long, p H 4-7 linear immobilized p H gradient(IPG) strips, and gels were stained with silver and coomassie brilliant blue. We identified 944 proteins expressed in RBSDV infected maize leaves by proteomics approaches. Among these, 44 protein spots that revealed a 1.5-fold difference in intensity were identified by mass spectrometry between mock-inoculated and RBSDV infected samples. Among these, 17 and 26 spots were up-regulated, and 27 and 18 spots were down-regulated in the virus infected samples of Zheng 58 and LN 287, respectively. Differential protein spots were analyzed by mass spectrometry identification, which could be divided into six categories. Furthermore, the expression of stress-related proteins was detected and confirmed by q RT-PCR. This study lays the foundation for further investigations, enabling the enhancement of MRDD resistance in maize.展开更多
基金This work was financially supported by the National Key Research and Development Program of China(2016YFD0102100 and 2016YFD0101802)the National Natural Science Foundation of China(31801346)the earmarked fund for China Agriculture Research System(CARS-03).
文摘Low stature in wheat is closely associated with lodging resistance,and this impacts harvest index and grain yield.The discovery of novel dwarfing or semi-dwarfing genes can have great significance for dwarf wheat breeding.In this study,we identified an EMS induced dwarf wheat mutant JE0124 from the elite cultivar Jing411.JE0124 possesses increased stem strength and a 33%reduction in plant height compared with wild type.Giberelic acid(GA)treatment analysis suggested that JE0124 was GA-sensitive.Analysis of the frequency distribution of plant height in four F,populations derived from crosses between JE0124 and the relatively taller varieties Nongda 5181 and WT indicated that the dwarfism phenotype was quantitatively inherited.We used two F,populations and 312 individuals from the reciprocal cross of Nongda 5181 and JE0124 to map the quantitative trait locus(QTL)for reduced height to a 0.85-cM interval on chromosome 2DL.The mapping was done by using a combination of 660K SNP array-based bulked segregant analysis(BSA)and genetic linkage analysis,with logarithm of odds(LOD)scores of 5.34 and 5.78,respectively.Additionally,this QTL accounted for 8.27-8.52%of the variation in the phenotype.The dwarf mutant JE0124 and the newly discovered dwarfing gene on chromosome 2DL in this study will enrich genetic resources for dwarf wheat breeding.
基金funded by the National Natural Science Foundation of China(31371636)the Modern Agricultural System of Shandong Province,China(SDAIT-01-022-01)+1 种基金the Key Research and Development Project of Shandong Province,China(2016GNC110018)the Applied Basic Research Project of Qingdao,China(14-2-4-13-jch)
文摘Maize rough dwarf disease(MRDD) is a viral disease caused by brown planthopper infestation, and leads to great yield loss, especially in China. Comparative proteomics was performed using maize inbred line Zheng 58 and LN 287. MRDD pathogen was detected as rice black-streaked dwarf virus(RBSDV) by quantitative real time PCR(q RT-PCR) in Shandong Province, China. The modified trichloroacetic acid(TCA)/acetone method was used for soluble protein extraction from leaves. Two-dimensional electrophoresis(2-DE) analysis was performed on 24-cm long, p H 4-7 linear immobilized p H gradient(IPG) strips, and gels were stained with silver and coomassie brilliant blue. We identified 944 proteins expressed in RBSDV infected maize leaves by proteomics approaches. Among these, 44 protein spots that revealed a 1.5-fold difference in intensity were identified by mass spectrometry between mock-inoculated and RBSDV infected samples. Among these, 17 and 26 spots were up-regulated, and 27 and 18 spots were down-regulated in the virus infected samples of Zheng 58 and LN 287, respectively. Differential protein spots were analyzed by mass spectrometry identification, which could be divided into six categories. Furthermore, the expression of stress-related proteins was detected and confirmed by q RT-PCR. This study lays the foundation for further investigations, enabling the enhancement of MRDD resistance in maize.
文摘FARs为脂酰辅酶A还原酶,在植物生长发育和抵御非生物胁迫过程中起着重要作用。为进一步研究FARs在玉米中行使的生物学功能,在玉米中克隆1个脂酰辅酶A还原酶1基因(ZmFAR1),该基因全长1883 bp,开放阅读框长1491 bp,编码496个氨基酸,分子量为55.983 k Da。生物信息学分析表明,ZmFAR1蛋白的分子式为C_(2522)H_(4010)N^(690)O_(701)S_(24),为稳定的碱性亲水蛋白,存在59个磷酸化位点,未发现信号肽,存在跨膜结构域,最大可能定位于细胞质。氨基酸残基组成中主要以α螺旋和无规则卷曲为主。系统进化树分析表明,ZmFAR1蛋白与南荻和高粱的FAR蛋白相似度最高。利用实时荧光定量PCR技术对ZmFAR1在模拟盐、干旱两种非生物胁迫条件下对根、茎、叶3种组织的表达模式进行研究,结果表明,ZmFAR1呈现组织特异性表达,在叶中的表达量最高。盐和干旱处理下,ZmFAR1的表达量出现明显变化,推测ZmFAR1可能在不同程度参与了玉米对非生物胁迫的应答。
