A laboratory experiment was conducted to assess the bioaccumulation of Pb^2＋ and its effects on growth, morphology and pigment contents of Spirulina （Arthrosl）ira）platensis. The specimen cultured in Zarrouk liquid...A laboratory experiment was conducted to assess the bioaccumulation of Pb^2＋ and its effects on growth, morphology and pigment contents of Spirulina （Arthrosl）ira）platensis. The specimen cultured in Zarrouk liquid medium was treated with various initial metal concentrations （0, 5, 10, 30, 50 and 10μgmL^-1）. The growth ofS. platensis was adversely affected by Pb〉 at high concentrations （30, 50 and 100μgmL^-1）. However, at low concentrations （5μgmL^-1）, Pb^2＋ could stimulate its growth slightly. The pigment contents （chlorophyllα and β carotene） were decreased in a dose-dependent manner. The highest reductions （67% and 53% respectively in chlorophyll α andβ carotene） were observed in 100 μg mL^-1 treatment group. The LC50 （96 h） of Pb^2- was measured as 75.34 μg mL^-1. Apart from a few cases of filament breakages at elevated concentrations （50 and 100 μgmL^-1）, morphological abnormalities are not specific. Metal bioaccumulation increased with Pb^2- concentrations, but decreased with exposure time. The maximum accumulated amount was 188 mgg^-1 dry weight. The bioconcentration factor （BCF） reached to a peak at day 2, followed by a gradual reduction tbr all the exposure concentrations. S. platensis is able to tolerate considerably high Pb^2＋ concentrations. Consequently it can be used as a potential species to remove heavy metal from contaminated waters.展开更多
基金the Chinese Scholarship Council is greatly acknowledged
文摘A laboratory experiment was conducted to assess the bioaccumulation of Pb^2＋ and its effects on growth, morphology and pigment contents of Spirulina （Arthrosl）ira）platensis. The specimen cultured in Zarrouk liquid medium was treated with various initial metal concentrations （0, 5, 10, 30, 50 and 10μgmL^-1）. The growth ofS. platensis was adversely affected by Pb〉 at high concentrations （30, 50 and 100μgmL^-1）. However, at low concentrations （5μgmL^-1）, Pb^2＋ could stimulate its growth slightly. The pigment contents （chlorophyllα and β carotene） were decreased in a dose-dependent manner. The highest reductions （67% and 53% respectively in chlorophyll α andβ carotene） were observed in 100 μg mL^-1 treatment group. The LC50 （96 h） of Pb^2- was measured as 75.34 μg mL^-1. Apart from a few cases of filament breakages at elevated concentrations （50 and 100 μgmL^-1）, morphological abnormalities are not specific. Metal bioaccumulation increased with Pb^2- concentrations, but decreased with exposure time. The maximum accumulated amount was 188 mgg^-1 dry weight. The bioconcentration factor （BCF） reached to a peak at day 2, followed by a gradual reduction tbr all the exposure concentrations. S. platensis is able to tolerate considerably high Pb^2＋ concentrations. Consequently it can be used as a potential species to remove heavy metal from contaminated waters.
