In order to improve the therapeutic index and reduce the side effects of As2O3 so as to expand its clinical utility to solid tumors,As2O3-loaded polylactic acid（PLA）/magnetic nanoparticles（PAMNs） were prepared and...In order to improve the therapeutic index and reduce the side effects of As2O3 so as to expand its clinical utility to solid tumors,As2O3-loaded polylactic acid（PLA）/magnetic nanoparticles（PAMNs） were prepared and the preparation conditions were optimized by the improved double emulsion solvent-evaporation method to obtain as a drug delivery system with a high As2O3 encapsulation efficiency and homogeneous nanosized particles.Successful loading of magnetic nanoparticles into the nanoparticles was confirmed by Fourier transform infrared（FTIR） spectroscopy.The drug loading,encapsulation efficiency and releasing properties of As2O3 in PAMNs were investigated by atomic fluorescence spectrometer analysis.Subsequently,the in vitro antitumor efficacy of free As2O3 and As2O3-PAMNs against SMMC-7721 cell line was investigated.Finally,confocal fluorescence microscopy was conducted to reveal the celluar uptake of As2O3-loaded PAMNs by SMMC-7721 cell line.展开更多
基金Supported by the National Natural Science Foundation of China（Nos.21201149,51273171）,the Natural Science Foundation of Jiangsu Province,China（No.BK2012259）,the Jiangsu College Graduate Research and Innovation Programs,China （No.CXLX12_0914）,the Project of the Testing Center of Yangzhou University（China） and the Priority Academic Program Development of Jiangsu Higher Education Institutions（China）.
文摘In order to improve the therapeutic index and reduce the side effects of As2O3 so as to expand its clinical utility to solid tumors,As2O3-loaded polylactic acid（PLA）/magnetic nanoparticles（PAMNs） were prepared and the preparation conditions were optimized by the improved double emulsion solvent-evaporation method to obtain as a drug delivery system with a high As2O3 encapsulation efficiency and homogeneous nanosized particles.Successful loading of magnetic nanoparticles into the nanoparticles was confirmed by Fourier transform infrared（FTIR） spectroscopy.The drug loading,encapsulation efficiency and releasing properties of As2O3 in PAMNs were investigated by atomic fluorescence spectrometer analysis.Subsequently,the in vitro antitumor efficacy of free As2O3 and As2O3-PAMNs against SMMC-7721 cell line was investigated.Finally,confocal fluorescence microscopy was conducted to reveal the celluar uptake of As2O3-loaded PAMNs by SMMC-7721 cell line.
