为鉴别及检测不同地区市售羊乳粉的品质,基于反相高效液相色谱(reversed-phase high performance liquid chromatography,RP-HPLC)技术,将35种市售羊乳粉根据陕西地区和非陕西地区、纯羊乳粉和配方羊乳粉进行分类与检测,研究不同产地、...为鉴别及检测不同地区市售羊乳粉的品质,基于反相高效液相色谱(reversed-phase high performance liquid chromatography,RP-HPLC)技术,将35种市售羊乳粉根据陕西地区和非陕西地区、纯羊乳粉和配方羊乳粉进行分类与检测,研究不同产地、不同种类羊乳粉的差异,对羊乳粉品质进行分析判别。结果表明:对市售羊乳粉乳蛋白进行RP-HPLC测定发现,配方羊乳粉α_(s2)-酪蛋白(α_(s2)-casein,α_(s2)-CN)和β-CN相对含量分别低于纯羊乳粉20%和24%,κ-CN和乳清蛋白相对含量分别高于纯羊乳粉30%和50%;不同地区纯羊乳粉乳蛋白组成及含量相似。因此,通过RP-HPLC可以实现对市售羊乳粉中乳蛋白的测定和质量评价。展开更多
As a less time-consuming procedure, subsampling technology has been widely used in biological monitoring and assessment programs. It is clear that subsampling counts af fect the value of traditional biodiversity indic...As a less time-consuming procedure, subsampling technology has been widely used in biological monitoring and assessment programs. It is clear that subsampling counts af fect the value of traditional biodiversity indices, but its ef fect on taxonomic distinctness(TD) indices is less well studied. Here, we examined the responses of traditional(species richness, Shannon-Wiener diversity) and TD(average taxonomic distinctness: Δ +, and variation in taxonomic distinctness: Λ +) indices to subsample counts using a random subsampling procedure from 50 to 400 individuals, based on macroinvertebrate datasets from three dif ferent river systems in China. At regional scale, taxa richness asymptotically increased with ?xed-count size; ≥250–300 individuals to express 95% information of the raw data. In contrast, TD indices were less sensitive to the subsampling procedure. At local scale, TD indices were more stable and had less deviation than species richness and Shannon-Wiener index, even at low subsample counts, with ≥100 individuals needed to estimate 95% of the information of the actual Δ + and Λ + in the three river basins. We also found that abundance had a certain ef fect on diversity indices during the subsampling procedure, with dif ferent subsampling counts for species richness and TD indices varying by regions. Therefore, we suggest that TD indices are suitable for biodiversity assessment and environment monitoring. Meanwhile, pilot analyses are necessary when to determine the appropriate subsample counts for bioassessment in a new region or habitat type.展开更多
文摘为鉴别及检测不同地区市售羊乳粉的品质,基于反相高效液相色谱(reversed-phase high performance liquid chromatography,RP-HPLC)技术,将35种市售羊乳粉根据陕西地区和非陕西地区、纯羊乳粉和配方羊乳粉进行分类与检测,研究不同产地、不同种类羊乳粉的差异,对羊乳粉品质进行分析判别。结果表明:对市售羊乳粉乳蛋白进行RP-HPLC测定发现,配方羊乳粉α_(s2)-酪蛋白(α_(s2)-casein,α_(s2)-CN)和β-CN相对含量分别低于纯羊乳粉20%和24%,κ-CN和乳清蛋白相对含量分别高于纯羊乳粉30%和50%;不同地区纯羊乳粉乳蛋白组成及含量相似。因此,通过RP-HPLC可以实现对市售羊乳粉中乳蛋白的测定和质量评价。
基金Supported by the National Natural Science Foundation of China(Nos.31400469,41571495,31770460)the National Science and Technology Basic Research Program(No.2015FY110400-4)+2 种基金the China Three Gorges Corporation Research Project(No.JGJ/0272015)the Key Program of the Chinese Academy of Sciences(Comprehensive Assessment Technology of River Ecology and Environment for the Water Source Region of "South-toNorth Water Diversion Central Route")the Program for Biodiversity Protection(No.2017HB2096001006)
文摘As a less time-consuming procedure, subsampling technology has been widely used in biological monitoring and assessment programs. It is clear that subsampling counts af fect the value of traditional biodiversity indices, but its ef fect on taxonomic distinctness(TD) indices is less well studied. Here, we examined the responses of traditional(species richness, Shannon-Wiener diversity) and TD(average taxonomic distinctness: Δ +, and variation in taxonomic distinctness: Λ +) indices to subsample counts using a random subsampling procedure from 50 to 400 individuals, based on macroinvertebrate datasets from three dif ferent river systems in China. At regional scale, taxa richness asymptotically increased with ?xed-count size; ≥250–300 individuals to express 95% information of the raw data. In contrast, TD indices were less sensitive to the subsampling procedure. At local scale, TD indices were more stable and had less deviation than species richness and Shannon-Wiener index, even at low subsample counts, with ≥100 individuals needed to estimate 95% of the information of the actual Δ + and Λ + in the three river basins. We also found that abundance had a certain ef fect on diversity indices during the subsampling procedure, with dif ferent subsampling counts for species richness and TD indices varying by regions. Therefore, we suggest that TD indices are suitable for biodiversity assessment and environment monitoring. Meanwhile, pilot analyses are necessary when to determine the appropriate subsample counts for bioassessment in a new region or habitat type.