Obiective:To investigate whether"Fuzheng Qingretonglin"decoction can reduce urinary tract damage caused by complex urinary tract infection caused by drug resistant Escherichia coli by regulating Nod-like rec...Obiective:To investigate whether"Fuzheng Qingretonglin"decoction can reduce urinary tract damage caused by complex urinary tract infection caused by drug resistant Escherichia coli by regulating Nod-like receptor pyrin domain3 inflammasome,and to explore the feasibility of this decoction combined with levofloxacin in the treatment of complex urinary tract infection caused by drug resistant bacteria.Methods:SD rats were divided into five groups:sham group,model group,levofloxacin group(Lev group),levofloxacin+Fuzheng Qingre Tonglin decoction group(FZ+lev group),and Fuzheng Qingre Tonglin decoction group(FZQRTL group).After the experiment,urine was taken for bacterial culture to determine the urinary tract infection of rats in each group;HE staining was used to observe the pathological changes of kidney and bladder tissues in rats;The expression of NLRP3 in kidney and bladder tissues was detected by immunohistochemistry;The expression of IL-1βand IL-18 in serum of rats was detected by ELISA;The expressions of NLRP3,ASC and Caspase-1 were detected by Western blotting.Results:The positive rate of urine bacteria culture in the sham group was 0%,the positive rate of urine bacteria culture in the model group was 100%;and the positive rate of urine bacteria culture in the FZ+lev group was 37.50%,which was statistically different from that in the model group(P<0.05).A large number of inflammatory cells were observed in the kidney and bladder tissues of the model group by HE staining,while the number of inflammatory cells in the kidney and bladder tissues of the Lev group and FZQRTL group was significantly reduced compared with that of the model group.The FZ+lev group in the number and structure of inflammatory cells in kidney and bladder were similar to the sham group.The NLRP3 immunohistochemistry of kidney and bladder tissue in FZ+lev groups and FZQRTL groups was significantly different from that in model group(P<0.001).The levels of IL-1βand IL-18 in serum of Lev group,FZQRTL group and FZ+lev group were significantly decreased by ELISA compared with model group(P<0.001).The levels of IL-1βand IL-18 in the FZ+lev groups were significantly lower than in the Lev group and FZQRTL group,and the differences were statistically significant(P<0.05).The protein expressions of NLRP3,ASC and Caspase-1 in the Lev group,FZQRTL group and FZ+lev group were significantly lower than those in the model group(P<0.001).The protein expressions of NLRP3,ASC and Caspase-1 in the FZ+lev groups were significantly lower than in the Lev group and FZQRTL group,and the differences were statistically significant(P<0.05).Conclusions:"Fuzheng Qingretonglin"decoction may have a protective effect on the kidney and bladder of rats with complex urinary tract infection caused by drug-resistant Escherichia coli by inhibiting the activation of NLRP3 inflammatory bodies,and TCM combined with levofloxacin has a better therapeutic effect than TCM or levofloxacin alone.展开更多
以山麻鸭为试验对象,研究在笼养条件下不同的采精频率对精液品质的影响及稀释液种类、输精量和输精间隔对山麻鸭受精率和孵化率的影响。选用山麻鸭公鸭30羽双笼饲养,随机平均分成3组,每组10羽,分别按间隔0 d、1 d(即次日、隔日采精),2 ...以山麻鸭为试验对象,研究在笼养条件下不同的采精频率对精液品质的影响及稀释液种类、输精量和输精间隔对山麻鸭受精率和孵化率的影响。选用山麻鸭公鸭30羽双笼饲养,随机平均分成3组,每组10羽,分别按间隔0 d、1 d(即次日、隔日采精),2 d采精,每组共采集4次精液并进行品质鉴定分析。母鸭384羽双笼饲养,随机分成6个试验组,每小组64羽,在得出最佳公鸭采精频率的基础上,采用不同的稀释液与输精量:原精液(0.01 m L、0.02 m L)、生理盐水和稀释液III(10倍稀释,输精量:0.1 m L、0.2 m L),间隔3 d输精,得出最佳的稀释液与输精量。再设计以输精间隔2,3,4,5,6,7 d为参数,探究最佳的输精间隔。结果表明:采精频率为间隔2 d时,可获得较好的精液品质。在进行授精时,使用生理盐水10倍稀释精液,输精量0.1 m L/羽,间隔3 d输精的情况下可获得最优的受精率和孵化率。由此可见,笼养山麻鸭最佳的采精频率为间隔2 d,且可采用生理盐水10倍稀释、输精量0.1 m L/羽、间隔3 d授精的输精参数。展开更多
基金Luzhou People's Government-Science and technology Strategic Cooperation project of Southwest Medical University(2017LZXNYD-T09)。
文摘Obiective:To investigate whether"Fuzheng Qingretonglin"decoction can reduce urinary tract damage caused by complex urinary tract infection caused by drug resistant Escherichia coli by regulating Nod-like receptor pyrin domain3 inflammasome,and to explore the feasibility of this decoction combined with levofloxacin in the treatment of complex urinary tract infection caused by drug resistant bacteria.Methods:SD rats were divided into five groups:sham group,model group,levofloxacin group(Lev group),levofloxacin+Fuzheng Qingre Tonglin decoction group(FZ+lev group),and Fuzheng Qingre Tonglin decoction group(FZQRTL group).After the experiment,urine was taken for bacterial culture to determine the urinary tract infection of rats in each group;HE staining was used to observe the pathological changes of kidney and bladder tissues in rats;The expression of NLRP3 in kidney and bladder tissues was detected by immunohistochemistry;The expression of IL-1βand IL-18 in serum of rats was detected by ELISA;The expressions of NLRP3,ASC and Caspase-1 were detected by Western blotting.Results:The positive rate of urine bacteria culture in the sham group was 0%,the positive rate of urine bacteria culture in the model group was 100%;and the positive rate of urine bacteria culture in the FZ+lev group was 37.50%,which was statistically different from that in the model group(P<0.05).A large number of inflammatory cells were observed in the kidney and bladder tissues of the model group by HE staining,while the number of inflammatory cells in the kidney and bladder tissues of the Lev group and FZQRTL group was significantly reduced compared with that of the model group.The FZ+lev group in the number and structure of inflammatory cells in kidney and bladder were similar to the sham group.The NLRP3 immunohistochemistry of kidney and bladder tissue in FZ+lev groups and FZQRTL groups was significantly different from that in model group(P<0.001).The levels of IL-1βand IL-18 in serum of Lev group,FZQRTL group and FZ+lev group were significantly decreased by ELISA compared with model group(P<0.001).The levels of IL-1βand IL-18 in the FZ+lev groups were significantly lower than in the Lev group and FZQRTL group,and the differences were statistically significant(P<0.05).The protein expressions of NLRP3,ASC and Caspase-1 in the Lev group,FZQRTL group and FZ+lev group were significantly lower than those in the model group(P<0.001).The protein expressions of NLRP3,ASC and Caspase-1 in the FZ+lev groups were significantly lower than in the Lev group and FZQRTL group,and the differences were statistically significant(P<0.05).Conclusions:"Fuzheng Qingretonglin"decoction may have a protective effect on the kidney and bladder of rats with complex urinary tract infection caused by drug-resistant Escherichia coli by inhibiting the activation of NLRP3 inflammatory bodies,and TCM combined with levofloxacin has a better therapeutic effect than TCM or levofloxacin alone.
文摘以山麻鸭为试验对象,研究在笼养条件下不同的采精频率对精液品质的影响及稀释液种类、输精量和输精间隔对山麻鸭受精率和孵化率的影响。选用山麻鸭公鸭30羽双笼饲养,随机平均分成3组,每组10羽,分别按间隔0 d、1 d(即次日、隔日采精),2 d采精,每组共采集4次精液并进行品质鉴定分析。母鸭384羽双笼饲养,随机分成6个试验组,每小组64羽,在得出最佳公鸭采精频率的基础上,采用不同的稀释液与输精量:原精液(0.01 m L、0.02 m L)、生理盐水和稀释液III(10倍稀释,输精量:0.1 m L、0.2 m L),间隔3 d输精,得出最佳的稀释液与输精量。再设计以输精间隔2,3,4,5,6,7 d为参数,探究最佳的输精间隔。结果表明:采精频率为间隔2 d时,可获得较好的精液品质。在进行授精时,使用生理盐水10倍稀释精液,输精量0.1 m L/羽,间隔3 d输精的情况下可获得最优的受精率和孵化率。由此可见,笼养山麻鸭最佳的采精频率为间隔2 d,且可采用生理盐水10倍稀释、输精量0.1 m L/羽、间隔3 d授精的输精参数。
