Background Nitric oxide （NO） synthesized by endothelial nitric oxide synthase （eNOS） plays an important role in both the regulation of endothelial function and the control of blood pressure. Up to now, there has b...Background Nitric oxide （NO） synthesized by endothelial nitric oxide synthase （eNOS） plays an important role in both the regulation of endothelial function and the control of blood pressure. Up to now, there has been conflicting data regarding the association between three clinically relevant polymorphisms （T-786C, intron4b/a and G894T） of the eNOS gene and essential hypertension.展开更多
Background Hypertension is a complex biological trait that influenced by multiple factors. The encouraging results for hypertension research showed that the linkage analysis can be used to replicate other studies and ...Background Hypertension is a complex biological trait that influenced by multiple factors. The encouraging results for hypertension research showed that the linkage analysis can be used to replicate other studies and discover new genetic risk factors. Previous studies linked human chromosome 14 to essential hypertension or blood pressure traits. With a Chinese population, we tried to replicate these findings.Methods A linkage about lO centi Morgen analysis and exclusion was performed with the scan was performed on chromosome 14 with 14-microsatellite markers with a density of （cM） in 147 Chinese hypertensive nuclear families. Muhipoint non-parametric linkage mapping were performed with the GENEHUNTER software, whereas quantitative analysis variance component method integrated in the SOLAR package.Results In the qualitative analysis, the highest non-parametric linkage score is 1.0 （ P=0.14） at DI4S261 in the single point analysis, and no loci achieved non-parametric linkage score more than 1.0 in the multipoint analysis. Maximum-likelihood mapping showed no significant results, either. Subsequently the traditional exclusion criteria of the log-of-the-odds score-2 were adopted, and the chromosome 14 with λs≥ 2.4 was excluded. In the quantitative analysis of blood pressure with the SOLAR software, two-point analysis and multipoint analysis suggested no evidence for linkage occurred on chromosome 14 for systolic and diastolic blood pressure.Conclusion There was no substantial evidence to support the linkage of chromosome 14 and essential hypertension or blood pressure trait in Chinese hypertensive subjects in this study.展开更多
基金This work was funded by grants of the National Tenth Five-year Plan Key Programs from the Ministry of Science and Technology of People's Republic of China (No. 2002BA711A05, 2002BA711A08 and 2002BA711A10)grant of biomedical project from the Council of Science and Technology, Beijing (No.H020220030130) grant of National Natural Science Foundation of China (No. 30270733).
文摘Background Nitric oxide （NO） synthesized by endothelial nitric oxide synthase （eNOS） plays an important role in both the regulation of endothelial function and the control of blood pressure. Up to now, there has been conflicting data regarding the association between three clinically relevant polymorphisms （T-786C, intron4b/a and G894T） of the eNOS gene and essential hypertension.
文摘Background Hypertension is a complex biological trait that influenced by multiple factors. The encouraging results for hypertension research showed that the linkage analysis can be used to replicate other studies and discover new genetic risk factors. Previous studies linked human chromosome 14 to essential hypertension or blood pressure traits. With a Chinese population, we tried to replicate these findings.Methods A linkage about lO centi Morgen analysis and exclusion was performed with the scan was performed on chromosome 14 with 14-microsatellite markers with a density of （cM） in 147 Chinese hypertensive nuclear families. Muhipoint non-parametric linkage mapping were performed with the GENEHUNTER software, whereas quantitative analysis variance component method integrated in the SOLAR package.Results In the qualitative analysis, the highest non-parametric linkage score is 1.0 （ P=0.14） at DI4S261 in the single point analysis, and no loci achieved non-parametric linkage score more than 1.0 in the multipoint analysis. Maximum-likelihood mapping showed no significant results, either. Subsequently the traditional exclusion criteria of the log-of-the-odds score-2 were adopted, and the chromosome 14 with λs≥ 2.4 was excluded. In the quantitative analysis of blood pressure with the SOLAR software, two-point analysis and multipoint analysis suggested no evidence for linkage occurred on chromosome 14 for systolic and diastolic blood pressure.Conclusion There was no substantial evidence to support the linkage of chromosome 14 and essential hypertension or blood pressure trait in Chinese hypertensive subjects in this study.
