Objective:To study the effect of maternal BDE-209 (brominated Diphenyl Ethers-209)exposure on the expression of microtubule-associated protein-1b (map-1b) and S-100 in rat's hippocampus of the offspring by RT-PCR....Objective:To study the effect of maternal BDE-209 (brominated Diphenyl Ethers-209)exposure on the expression of microtubule-associated protein-1b (map-1b) and S-100 in rat's hippocampus of the offspring by RT-PCR.Methods:Peanut oil suspensions of commercial deca-BDE was given in dose of 300 mg/(kg·d) by oral gavage throughout gestation and lactation in experimental group.The control group was administered only with the same capacity of peanut oil at the same time.The expression of MAP-1B in the hippocampus of the offspring's rats were tested when the pups were newborn,7days,14 days,21days and 45days old respectively by means of RT-PCR.Result:MAP-1B protein showed a statistically significantly lower concentration in the groups 14 days,21days,45days than that of the control groups.The expression of S-100 in the group which received with deca-BDE by RT-PCR showed higher than that of control groups.But only the 45days groups had significant difference of expression of MAP-1B protein compared with the control groups(P<0.05).Conclusions:Maternal BDE-209 exposure during the period of pregnancy will diminish the expression of map-1b protein in hippocampus of offspring's rats.展开更多
准确获取及预测光合色素含量可为精细化种植管理提供数据依据,为探究花生冠层叶片色素吸收特征,该研究以开花下针期的花生冠层叶片为研究对象,以ASD Field Spec4野外便携式高光谱仪采集的光谱数据为数据源,进行花生叶片叶绿素含量和类...准确获取及预测光合色素含量可为精细化种植管理提供数据依据,为探究花生冠层叶片色素吸收特征,该研究以开花下针期的花生冠层叶片为研究对象,以ASD Field Spec4野外便携式高光谱仪采集的光谱数据为数据源,进行花生叶片叶绿素含量和类胡萝卜素含量反演。通过对比7种单一筛选特征波长变量算法及结合4种模型(PLSR、SVR、GBDT和XGBoost)的结果,优选出3种算法进行两两耦合。结果表明:1)在单一算法试验中IRIV、UVE和GA算法结果较优;2)在耦合算法试验中UVE-IRIV、GA-IRIV和GA-UVE方法都能有效降维,且模型稳定性提升。在叶绿素含量反演模型中,GA-IRIV-XGBoost模型精度最高,R^(2)=0.622,RMSE=0.235 mg/g;在类胡萝卜素含量反演模型中,UVE-IRIV-XGBoost模型精度最高,R^(2)=0.575,RMSE=0.056 mg/g;3)比较两种色素反演模型的预测精度,表明叶绿素的预测精度优于类胡萝卜素。该结果可为快速、准确预测花生叶片光合色素含量提供一种方法。展开更多
文摘Objective:To study the effect of maternal BDE-209 (brominated Diphenyl Ethers-209)exposure on the expression of microtubule-associated protein-1b (map-1b) and S-100 in rat's hippocampus of the offspring by RT-PCR.Methods:Peanut oil suspensions of commercial deca-BDE was given in dose of 300 mg/(kg·d) by oral gavage throughout gestation and lactation in experimental group.The control group was administered only with the same capacity of peanut oil at the same time.The expression of MAP-1B in the hippocampus of the offspring's rats were tested when the pups were newborn,7days,14 days,21days and 45days old respectively by means of RT-PCR.Result:MAP-1B protein showed a statistically significantly lower concentration in the groups 14 days,21days,45days than that of the control groups.The expression of S-100 in the group which received with deca-BDE by RT-PCR showed higher than that of control groups.But only the 45days groups had significant difference of expression of MAP-1B protein compared with the control groups(P<0.05).Conclusions:Maternal BDE-209 exposure during the period of pregnancy will diminish the expression of map-1b protein in hippocampus of offspring's rats.
文摘准确获取及预测光合色素含量可为精细化种植管理提供数据依据,为探究花生冠层叶片色素吸收特征,该研究以开花下针期的花生冠层叶片为研究对象,以ASD Field Spec4野外便携式高光谱仪采集的光谱数据为数据源,进行花生叶片叶绿素含量和类胡萝卜素含量反演。通过对比7种单一筛选特征波长变量算法及结合4种模型(PLSR、SVR、GBDT和XGBoost)的结果,优选出3种算法进行两两耦合。结果表明:1)在单一算法试验中IRIV、UVE和GA算法结果较优;2)在耦合算法试验中UVE-IRIV、GA-IRIV和GA-UVE方法都能有效降维,且模型稳定性提升。在叶绿素含量反演模型中,GA-IRIV-XGBoost模型精度最高,R^(2)=0.622,RMSE=0.235 mg/g;在类胡萝卜素含量反演模型中,UVE-IRIV-XGBoost模型精度最高,R^(2)=0.575,RMSE=0.056 mg/g;3)比较两种色素反演模型的预测精度,表明叶绿素的预测精度优于类胡萝卜素。该结果可为快速、准确预测花生叶片光合色素含量提供一种方法。
