The presence of microplastics in aquatic ecosystems is of increasing global concern.Nano-sized plastics,in particular,can penetrate the cell membrane and cause biological death.Our study evaluated the combined impacts...The presence of microplastics in aquatic ecosystems is of increasing global concern.Nano-sized plastics,in particular,can penetrate the cell membrane and cause biological death.Our study evaluated the combined impacts of several polystyrene mi-crospheres’sizes and nominal concentrations on the overall performance changes of Brachionus plicatilis.Experimental animals were exposed to three microplastic sizes(0.08,0.5 and 6μm)and five nominal concentrations(0,0.5,2,8,32μg mL−1)for 20 d.Our results showed that the toxicological effect of particle size on rotifers did not significantly depend on the nominal concentration.The interaction between the nominal concentration and size occurred only for body length and lorica width.Specifically,high nominal concentrations of microplastics that were close to nanometer size significantly impaired the overall vitality of rotifers,embodied in shortage of body type,delay in the arrival of maturity,reduction in the cumulative number of neonates,and the advance of the death process.In comparison,fair-sized size(0.5 and 6μm)displayed non-significant damage except for individual groups.Most notably,the net reproductive yield was only a third of what it was in the original environment,implying that there was not much fertility left.Besides,with the development of rotifers,the adverse effects of polystyrene microsphere drive had become more and more serious.展开更多
Anthropogenic emission of atmospheric carbon dioxide (CO2) has led to a rapid increase in atmospheric CO2 concentra- tion. Increasing atmospheric CO2 can reduce seawater pH and carbonate ions, which may adversely affe...Anthropogenic emission of atmospheric carbon dioxide (CO2) has led to a rapid increase in atmospheric CO2 concentra- tion. Increasing atmospheric CO2 can reduce seawater pH and carbonate ions, which may adversely affect the survival of the larvae of calcareous animals. Cyclina sinensis is a commercially and ecologically important species in several Asian countries. Living in coast shallow waters, this species has experienced the coastal environmental changes frequently throughout its life cycle. In this study, we simulated possible future seawater pH values including 8.2, 7.8 and 7.4 and examined the effects of ocean acidification on the early development of C. sinensis. Clam embryos were incubated for 48 h (2 d) in control and high-CO2 seawater to compare embryo- genesis, larval growth and swimming behavior. Fertilization rate was quite sensitive to pH, and moderate acidification could induce a significant decrease in fertilization rate. However, only extreme acidification could bring significant negative effect to hatching rate, body size, and average path velocity of trochophora. Moreover, with seawater acidification, C. sinensis needs much more time to reach the same developmental stage, which increases the risk of larva survival. Together with recent studies demonstrating negative impacts of high CO2 on fertilization and larva swimming behavior, the results imply a future decrease of C. sinensis populations in oceans if its acclimation to the predicted environmental alteration does not occur.展开更多
Nanoplastic(NP)contaminants are receiving increasing attention due to the harm they can cause to aquatic organisms and their accumulation in the human body through the food web.The goal of this study was to evaluate t...Nanoplastic(NP)contaminants are receiving increasing attention due to the harm they can cause to aquatic organisms and their accumulation in the human body through the food web.The goal of this study was to evaluate the oxidative stress caused by NPs on the clams Meretrix meretrix and Sinonovacula constricta.Specimens were exposed to three concentrations of fluorescently labeled polystyrene NPs(PS-NPs;0,0.1,and 1 mg L^(-1))for 7 d followed by a 3 d recovery period.The activities of antioxidant enzymes,such as catalase(CAT),superoxide dismutase(SOD),glutathione peroxidase(GPx)and the content of malonaldehyde(MDA)were measured in the digestive gland,gills,and mantle tissues.Results showed that the SOD activity in the gills of M.meretrix was significantly higher than that of the control at day 1,and PS-NPs exposure inhibited the CAT activity in the gills and SOD and GPx activities in the mantle.Exposure to low or high concentrations of PS-NPs increased the activities of both CAT and GPx in the gills of S.constricta at day 3.The oxidative damage was more severe in the digestive gland of M.meretrix and in the mantle of S.constricta based on MDA level,and it returned to normal after recovery.Integrated biomarker response,index version 2(IBRv2)values showed that the gills were more sensitive to PS-NPs than mantle and digestive gland.Therefore,the oxidative stress by PS-NPs depended on exposure time,dose,organ,and species.展开更多
Microplastics(MPs)pollution has become a worldwide environmental problem.The study about the effects of microplastics on fish,especially on the fish immune system is limited.In the present study,the transcriptome of s...Microplastics(MPs)pollution has become a worldwide environmental problem.The study about the effects of microplastics on fish,especially on the fish immune system is limited.In the present study,the transcriptome of soiny mullet(Liza haematocheila)larvae at 7 d and 14 d post 0.5μm MPs exposure were analyzed by DNBSEQ platform.A total number of 96585 unigenes were obtained with average length of 1925 bp.Approximately 72.48%(70004),76.80%(74176),and 62.94%(60787)unigenes were matched with the annotated sequences for non-redundant(NR),NCBI nucleotide(NT)and Swissprot database,respectively.Compared with control group,1336 differently expressed genes(DEGs)were identified at 7 d post MPs exposure.As the MPs exposure time extended to 14 d,the number of DEGs in the MPs exposure group reached to 3561.Further,KEGG enrichment analysis revealed that several immune-related pathways,such as interleukin(IL)-17 signaling pathway,tumor necrosis factor(TNF)signaling pathway,were affected by MPs exposure.In addition,apoptosis signaling pathway was also enriched under MPs exposure.These results demonstrated that MPs can alter the expressions of immune-related genes,providing basis for understanding the immune toxicity of MPs on fish and other vertebrates.展开更多
基金funded by research grants from the Na-tural Science Foundation of China(Nos.41706142 and 61936014)the National Modern Agricultural Industry Technology System Construction Project(No.CARS-49)the National Key R&D Program during the 13th Five-Year Plan Period(No.2018YFD900603).Dr.Yan-ming Sui is supported by a fellowship from China Schol-arship Council.
文摘The presence of microplastics in aquatic ecosystems is of increasing global concern.Nano-sized plastics,in particular,can penetrate the cell membrane and cause biological death.Our study evaluated the combined impacts of several polystyrene mi-crospheres’sizes and nominal concentrations on the overall performance changes of Brachionus plicatilis.Experimental animals were exposed to three microplastic sizes(0.08,0.5 and 6μm)and five nominal concentrations(0,0.5,2,8,32μg mL−1)for 20 d.Our results showed that the toxicological effect of particle size on rotifers did not significantly depend on the nominal concentration.The interaction between the nominal concentration and size occurred only for body length and lorica width.Specifically,high nominal concentrations of microplastics that were close to nanometer size significantly impaired the overall vitality of rotifers,embodied in shortage of body type,delay in the arrival of maturity,reduction in the cumulative number of neonates,and the advance of the death process.In comparison,fair-sized size(0.5 and 6μm)displayed non-significant damage except for individual groups.Most notably,the net reproductive yield was only a third of what it was in the original environment,implying that there was not much fertility left.Besides,with the development of rotifers,the adverse effects of polystyrene microsphere drive had become more and more serious.
基金supported by the Central Public-interest Scientific Institution Basal Research Fund (Nos. 2016HYZD0601 and 2018HYXKQ01-10)the Fund of Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, P. R. Chinathe National Modern Agricultural Industry Technology System Construction Project (No. CARS-49)
文摘Anthropogenic emission of atmospheric carbon dioxide (CO2) has led to a rapid increase in atmospheric CO2 concentra- tion. Increasing atmospheric CO2 can reduce seawater pH and carbonate ions, which may adversely affect the survival of the larvae of calcareous animals. Cyclina sinensis is a commercially and ecologically important species in several Asian countries. Living in coast shallow waters, this species has experienced the coastal environmental changes frequently throughout its life cycle. In this study, we simulated possible future seawater pH values including 8.2, 7.8 and 7.4 and examined the effects of ocean acidification on the early development of C. sinensis. Clam embryos were incubated for 48 h (2 d) in control and high-CO2 seawater to compare embryo- genesis, larval growth and swimming behavior. Fertilization rate was quite sensitive to pH, and moderate acidification could induce a significant decrease in fertilization rate. However, only extreme acidification could bring significant negative effect to hatching rate, body size, and average path velocity of trochophora. Moreover, with seawater acidification, C. sinensis needs much more time to reach the same developmental stage, which increases the risk of larva survival. Together with recent studies demonstrating negative impacts of high CO2 on fertilization and larva swimming behavior, the results imply a future decrease of C. sinensis populations in oceans if its acclimation to the predicted environmental alteration does not occur.
基金supported by the Natural Natural Science Foundation of China(No.41706142)the Earmarked Fund for CARS-49(No.CARS-49).
文摘Nanoplastic(NP)contaminants are receiving increasing attention due to the harm they can cause to aquatic organisms and their accumulation in the human body through the food web.The goal of this study was to evaluate the oxidative stress caused by NPs on the clams Meretrix meretrix and Sinonovacula constricta.Specimens were exposed to three concentrations of fluorescently labeled polystyrene NPs(PS-NPs;0,0.1,and 1 mg L^(-1))for 7 d followed by a 3 d recovery period.The activities of antioxidant enzymes,such as catalase(CAT),superoxide dismutase(SOD),glutathione peroxidase(GPx)and the content of malonaldehyde(MDA)were measured in the digestive gland,gills,and mantle tissues.Results showed that the SOD activity in the gills of M.meretrix was significantly higher than that of the control at day 1,and PS-NPs exposure inhibited the CAT activity in the gills and SOD and GPx activities in the mantle.Exposure to low or high concentrations of PS-NPs increased the activities of both CAT and GPx in the gills of S.constricta at day 3.The oxidative damage was more severe in the digestive gland of M.meretrix and in the mantle of S.constricta based on MDA level,and it returned to normal after recovery.Integrated biomarker response,index version 2(IBRv2)values showed that the gills were more sensitive to PS-NPs than mantle and digestive gland.Therefore,the oxidative stress by PS-NPs depended on exposure time,dose,organ,and species.
基金supported by the Major Projects of Natural Science Research for University and Colleges in Jiangsu Province(No.21KJA240001)the Key Research and Development Program of Jiangsu Province(No.BE2018348)+2 种基金partially by the Jiangsu Agricultural Science and Technology Innovation Fund(No.CX(22)3199)the Projects for the High-Quality Development of Fishery Industry of Yancheng City(No.YCSCYJ20210014)supported financially by the projects for‘Six Talents’of Jiangsu Province(No.NY115)。
文摘Microplastics(MPs)pollution has become a worldwide environmental problem.The study about the effects of microplastics on fish,especially on the fish immune system is limited.In the present study,the transcriptome of soiny mullet(Liza haematocheila)larvae at 7 d and 14 d post 0.5μm MPs exposure were analyzed by DNBSEQ platform.A total number of 96585 unigenes were obtained with average length of 1925 bp.Approximately 72.48%(70004),76.80%(74176),and 62.94%(60787)unigenes were matched with the annotated sequences for non-redundant(NR),NCBI nucleotide(NT)and Swissprot database,respectively.Compared with control group,1336 differently expressed genes(DEGs)were identified at 7 d post MPs exposure.As the MPs exposure time extended to 14 d,the number of DEGs in the MPs exposure group reached to 3561.Further,KEGG enrichment analysis revealed that several immune-related pathways,such as interleukin(IL)-17 signaling pathway,tumor necrosis factor(TNF)signaling pathway,were affected by MPs exposure.In addition,apoptosis signaling pathway was also enriched under MPs exposure.These results demonstrated that MPs can alter the expressions of immune-related genes,providing basis for understanding the immune toxicity of MPs on fish and other vertebrates.
