We theoretically investigate the hybrid plasmonic modes of cylindrical nanocables with gold nanocore and two dielectric nanolayers(SiO2 and BN).By solving a complete set of Maxwell's equations,the propagation cons...We theoretically investigate the hybrid plasmonic modes of cylindrical nanocables with gold nanocore and two dielectric nanolayers(SiO2 and BN).By solving a complete set of Maxwell's equations,the propagation constants and effective radii depending on geometrical parameters are numerically calculated.By declining a trade-off between propagation length and light confinement,high quality hybrid modes which can travel a long range of 120-200λwith a subwavelength effective radius are obtained at the optical wavelength.These modes in one-dimensional cylindrical waveguides should have potential applications in nanoscale optical device designs.展开更多
目的克隆、表达粉尘螨Der f 32蛋白,并鉴定其免疫原性及其对树突状细胞(DC)的调节作用。方法合成粉尘螨过敏原Der f 32基因,与pET-24a(+)载体连接,转入大肠埃希菌BL21诱导表达,经亲和层析法纯化后,十二烷基硫酸钠-聚丙烯酰氨凝胶电泳(SD...目的克隆、表达粉尘螨Der f 32蛋白,并鉴定其免疫原性及其对树突状细胞(DC)的调节作用。方法合成粉尘螨过敏原Der f 32基因,与pET-24a(+)载体连接,转入大肠埃希菌BL21诱导表达,经亲和层析法纯化后,十二烷基硫酸钠-聚丙烯酰氨凝胶电泳(SDS-PAGE)分析重组蛋白表达情况,蛋白质印迹(Western blotting)、临床皮肤点刺实验检测重组蛋白的免疫原性。将重组蛋白Der f 32与小鼠来源的DC2.4共培养24 h,设阳性对照组(加等量重组蛋白Der f 1)和阴性对照组(加等量PBS),流式细胞术检测细胞表面共刺激分子(CD40、 CD83和CD80)的表达。将重组蛋白稀释为10、 20、 30、 40、 50、 60μg/ml等不同浓度,分别与DC2.4共培养48 h后, Western blotting分析T细胞免疫球蛋白及黏蛋白域蛋白4 (TIM4)的表达情况。结果SDS-PAGE结果显示,重组蛋白Der f 32相对分子质量(Mr)约35 000。临床皮肤点刺实验表明, 42位尘螨过敏患者中有6例对重组蛋白Der f 32过敏,阳性率为14.2%。Western blotting结果显示,重组蛋白Der f 32能与皮试阳性患者血清IgE抗体特异性结合。流式细胞术检测结果显示,重组蛋白Der f 32与DC2.4共培养后, CD40、 CD83、 CD80的表达水平分别为24.5%、 9.6%和24.6%,高于阴性对照组(11.9%、 3.1%和15.6%)(P <0.01),低于阳性对照组(38.2%、 15.0%和39.1%)(P <0.05)。Western blotting检测结果显示, TIM4的表达量随重组蛋白Der f 32浓度的升高逐渐增加,且在Der f 32浓度为50μg/ml时, TIM4的表达量达到最高, TIM4相对灰度值为1.112。结论获得了具有较强免疫原性的粉尘螨新过敏原Der f 32,该蛋白能促进DC表面分子和TIM4的表达。展开更多
Background Cockroaches are an important indoor allergen source causing allergic rhinitis and asthma. The aim of this study was to investigate the cockroach prevalence in mainland of China and the cross-reactivity of I...Background Cockroaches are an important indoor allergen source causing allergic rhinitis and asthma. The aim of this study was to investigate the cockroach prevalence in mainland of China and the cross-reactivity of IgE between cockroach and house dust mite allergen in Chinese patients.Methods The cockroach sensitization pattern was based on a skin prick test （SPT） obtained from a national multicenter prevalence study, in which 6304 patients from 25 allergy centers across China participated. Factors, including different regions of China, age, gender and the correlations between the American and German cockroaches and house dust mite Der p were investigated. Eighteen out of 1236 clinical sera from south China were selected to perform the cross-inhibition assay between house dust mites and cockroaches.Results Totally 25.7% of patients were SPT positive to the American cockroach （Periplaneta Americana, Per a） and 18.7% SPT positive to the German cockroach （Blattella germanica, Bla g）. The prevalence of positive cockroach SPT was higher in southern than in northern China, higher in adults than in children, and higher in males than in females.Patients had relatively low levels of cockroach SPT reactions, mainly class 1 or 2. Of the SPT positive cockroach patients,88% were also SPT positive to house dust mite （Dermatophagoides pteronyssinus, Der p）. An IgE cross-inhibition study confirmed that Der P sensitization could cause false positive SPT reactions against cockroach.Conclusions A relatively high prevalence of cockroach sensitivity was found in mainland of China. However, a cross-inhibition study showed that only a small number of patients appear to have Bla g and/or Per a as primary sensitizing source. The importance of cockroaches as a risk factor for sensitization and triggers of allergic symptoms in mainland of China needs to be further investigated.展开更多
基金Supported by the National Natural Science Foundation of China under Grant Nos 10874004 and 10821062National Basic Research Program of China under Grant No 2007CB307001.
文摘We theoretically investigate the hybrid plasmonic modes of cylindrical nanocables with gold nanocore and two dielectric nanolayers(SiO2 and BN).By solving a complete set of Maxwell's equations,the propagation constants and effective radii depending on geometrical parameters are numerically calculated.By declining a trade-off between propagation length and light confinement,high quality hybrid modes which can travel a long range of 120-200λwith a subwavelength effective radius are obtained at the optical wavelength.These modes in one-dimensional cylindrical waveguides should have potential applications in nanoscale optical device designs.
文摘目的克隆、表达粉尘螨Der f 32蛋白,并鉴定其免疫原性及其对树突状细胞(DC)的调节作用。方法合成粉尘螨过敏原Der f 32基因,与pET-24a(+)载体连接,转入大肠埃希菌BL21诱导表达,经亲和层析法纯化后,十二烷基硫酸钠-聚丙烯酰氨凝胶电泳(SDS-PAGE)分析重组蛋白表达情况,蛋白质印迹(Western blotting)、临床皮肤点刺实验检测重组蛋白的免疫原性。将重组蛋白Der f 32与小鼠来源的DC2.4共培养24 h,设阳性对照组(加等量重组蛋白Der f 1)和阴性对照组(加等量PBS),流式细胞术检测细胞表面共刺激分子(CD40、 CD83和CD80)的表达。将重组蛋白稀释为10、 20、 30、 40、 50、 60μg/ml等不同浓度,分别与DC2.4共培养48 h后, Western blotting分析T细胞免疫球蛋白及黏蛋白域蛋白4 (TIM4)的表达情况。结果SDS-PAGE结果显示,重组蛋白Der f 32相对分子质量(Mr)约35 000。临床皮肤点刺实验表明, 42位尘螨过敏患者中有6例对重组蛋白Der f 32过敏,阳性率为14.2%。Western blotting结果显示,重组蛋白Der f 32能与皮试阳性患者血清IgE抗体特异性结合。流式细胞术检测结果显示,重组蛋白Der f 32与DC2.4共培养后, CD40、 CD83、 CD80的表达水平分别为24.5%、 9.6%和24.6%,高于阴性对照组(11.9%、 3.1%和15.6%)(P <0.01),低于阳性对照组(38.2%、 15.0%和39.1%)(P <0.05)。Western blotting检测结果显示, TIM4的表达量随重组蛋白Der f 32浓度的升高逐渐增加,且在Der f 32浓度为50μg/ml时, TIM4的表达量达到最高, TIM4相对灰度值为1.112。结论获得了具有较强免疫原性的粉尘螨新过敏原Der f 32,该蛋白能促进DC表面分子和TIM4的表达。
文摘Background Cockroaches are an important indoor allergen source causing allergic rhinitis and asthma. The aim of this study was to investigate the cockroach prevalence in mainland of China and the cross-reactivity of IgE between cockroach and house dust mite allergen in Chinese patients.Methods The cockroach sensitization pattern was based on a skin prick test （SPT） obtained from a national multicenter prevalence study, in which 6304 patients from 25 allergy centers across China participated. Factors, including different regions of China, age, gender and the correlations between the American and German cockroaches and house dust mite Der p were investigated. Eighteen out of 1236 clinical sera from south China were selected to perform the cross-inhibition assay between house dust mites and cockroaches.Results Totally 25.7% of patients were SPT positive to the American cockroach （Periplaneta Americana, Per a） and 18.7% SPT positive to the German cockroach （Blattella germanica, Bla g）. The prevalence of positive cockroach SPT was higher in southern than in northern China, higher in adults than in children, and higher in males than in females.Patients had relatively low levels of cockroach SPT reactions, mainly class 1 or 2. Of the SPT positive cockroach patients,88% were also SPT positive to house dust mite （Dermatophagoides pteronyssinus, Der p）. An IgE cross-inhibition study confirmed that Der P sensitization could cause false positive SPT reactions against cockroach.Conclusions A relatively high prevalence of cockroach sensitivity was found in mainland of China. However, a cross-inhibition study showed that only a small number of patients appear to have Bla g and/or Per a as primary sensitizing source. The importance of cockroaches as a risk factor for sensitization and triggers of allergic symptoms in mainland of China needs to be further investigated.
