Genetic segregation analysis for flag leaf angle was conducted using six generations of P1, P2, F1, B1, B2 and F2 derived from a cross of 863B （a maintainer line of japonica rice） and A7444 （a germplasm with large ...Genetic segregation analysis for flag leaf angle was conducted using six generations of P1, P2, F1, B1, B2 and F2 derived from a cross of 863B （a maintainer line of japonica rice） and A7444 （a germplasm with large flag leaf angle）. Genotypes and phenotypes of flag leaf angle were investigated in 863B （P1）, A7444 （P2） and 141 plants in BC^F~ （863BIA744411863B） population. An SSR genetic linkage map was constructed and QTLs for flag leaf angle were detected. The genetic map containing 79 information loci was constructed, which covers a total distance of 441.6 cM, averaging 5.6 cM between two neighboring loci. Results showed that the trait was controlled by two major genes plus polygene and the major genes were more important. Fifteen markers showed highly significant correlations with flag leaf angle based on single marker regression analysis. Two QTLs （qFLA2 and qFLA8） for flag leaf angle were detected by both composite interval method in software WinQTLCart 2.5 and composite interval method based on mixed linear model in QTL Network 2.0. The qFLA2 explained 10.50% and 13.28% of phenotypic variation, respectively, and was located at the interval of RM300 and RM145 on the short arm of chromosome 2. The qFLA8 explained 9.59% and 7.64% of phenotypic variation, respectively, and was located at the interval flanking RM6215 and RM8265 on the long arm of chromosome 8. The positive alleles at the two QTLs were both contributed from A7444.展开更多
Early generation stability of a japonica rice variety, 84-15, was retested by using the allelic differences of two qualitative traits, i.e., glume tip color and waxy endosperm. Results showed that both glurne tip colo...Early generation stability of a japonica rice variety, 84-15, was retested by using the allelic differences of two qualitative traits, i.e., glume tip color and waxy endosperm. Results showed that both glurne tip color and waxy endosperm segregated in progenies of single F1 plants derived from two crosses of 84-15/Zi 18 （purple color） and 84-15/Dahuaxiangnuo （waxy）. The results denied the conclusion obtained in previous studies that 84-15 had a characteristic of early generation stability. Moreover, it was found that using the coefficient of variation as an indicator of early generation stability was unreasonable by analyzing plant height as an example. Importance of avoiding systematic error in research works was also discussed.展开更多
基金supported by the National High Technology Research and Development Program of China(Grant No. 2010AA101300)the Platform Construction for Science and Technology Basic Condition from Science and Technology Ministry,China (Grant No.505005)
文摘Genetic segregation analysis for flag leaf angle was conducted using six generations of P1, P2, F1, B1, B2 and F2 derived from a cross of 863B （a maintainer line of japonica rice） and A7444 （a germplasm with large flag leaf angle）. Genotypes and phenotypes of flag leaf angle were investigated in 863B （P1）, A7444 （P2） and 141 plants in BC^F~ （863BIA744411863B） population. An SSR genetic linkage map was constructed and QTLs for flag leaf angle were detected. The genetic map containing 79 information loci was constructed, which covers a total distance of 441.6 cM, averaging 5.6 cM between two neighboring loci. Results showed that the trait was controlled by two major genes plus polygene and the major genes were more important. Fifteen markers showed highly significant correlations with flag leaf angle based on single marker regression analysis. Two QTLs （qFLA2 and qFLA8） for flag leaf angle were detected by both composite interval method in software WinQTLCart 2.5 and composite interval method based on mixed linear model in QTL Network 2.0. The qFLA2 explained 10.50% and 13.28% of phenotypic variation, respectively, and was located at the interval of RM300 and RM145 on the short arm of chromosome 2. The qFLA8 explained 9.59% and 7.64% of phenotypic variation, respectively, and was located at the interval flanking RM6215 and RM8265 on the long arm of chromosome 8. The positive alleles at the two QTLs were both contributed from A7444.
基金supported by the Program of Introducing Talents of Discipline to University,China(Grant No.B08025)
文摘Early generation stability of a japonica rice variety, 84-15, was retested by using the allelic differences of two qualitative traits, i.e., glume tip color and waxy endosperm. Results showed that both glurne tip color and waxy endosperm segregated in progenies of single F1 plants derived from two crosses of 84-15/Zi 18 （purple color） and 84-15/Dahuaxiangnuo （waxy）. The results denied the conclusion obtained in previous studies that 84-15 had a characteristic of early generation stability. Moreover, it was found that using the coefficient of variation as an indicator of early generation stability was unreasonable by analyzing plant height as an example. Importance of avoiding systematic error in research works was also discussed.
