首先归纳了多学科交叉实验室--致远创新研究中心的特点及管理难点;随后结合EHS(Environment,Health and Safety)管理体系,深入分析了多学科交叉实验室的安全管理机制及其特色,重点介绍线上、线下相结合的实验室安全教育与管理体系,最后...首先归纳了多学科交叉实验室--致远创新研究中心的特点及管理难点;随后结合EHS(Environment,Health and Safety)管理体系,深入分析了多学科交叉实验室的安全管理机制及其特色,重点介绍线上、线下相结合的实验室安全教育与管理体系,最后总结了实验室安全管理的实践经验,以期为我国新兴多学科交叉实验室的安全管理工作提供借鉴。展开更多
Enhancement of the SNR (signal to noise ratio) in single-molecule imaging is significantly important for improving image resolu-tion and distinguishing the fine structures of single molecules at a higher precision lev...Enhancement of the SNR (signal to noise ratio) in single-molecule imaging is significantly important for improving image resolu-tion and distinguishing the fine structures of single molecules at a higher precision level.Image processing techniques have dem-onstrated the remarkable capability to improve the SNR and the resolution level by breaking through some inherent limitations unresolved by instrument hardware optimization.In this paper, we focus on single-biomolecule imaging using atomic force mi-croscopy (AFM), a unique method in separated single-biomolecule imaging, and there was few suitable image processing tech-niques reported to increase the SNR of a single molecule’s AFM image.With the typical samples of separately dispersed DNA molecules, we replaced the classified averaging method, which is usually used when the molecules’ structure can be easily and repeatedly prepared, with the time averaging method to improve the SNR in a single molecule’s AFM imaging.Combining the time averaging technique with the image alignment method for the series of time-lapse AFM images of a single DNA molecule, we were able to improve the image’s SNR and recover some buried signals from the background noises.Furthermore, the fine structures of single molecules can potentially be further resolved if other image processing techniques are applied collaboratively with some newly developed imaging methods like Vibrating Mode Scanning Polarization Force Microscopy (VSPFM), and such combination will also be advantageous for the manipulation of single-biomolecules by AFM.In addition, the proposed algorithms for the estimations of drift, distortion and SNR are applicable for quantitative status characterization of AFM imaging.展开更多
Nanobubbles have been proven existent at the liquid/solid interface, and become a focus of research on varied interfacial processes. In the present work, by observing in situ with atomic force microscope (AFM), we fou...Nanobubbles have been proven existent at the liquid/solid interface, and become a focus of research on varied interfacial processes. In the present work, by observing in situ with atomic force microscope (AFM), we found that nanobubbles could influence the adsorption process of bovine serum albumin (BSA) on hydrophobic surface of highly ordered pyrolytic graphite (HOPG). BSA could adsorb evenly, and coexist with nanobubbles at water/HOPG interface. After removing nanobubbles by injecting ethanol, some hollows were found in the BSA layers at the same positions of nanobubbles existing previously. These hollows were about 8 nm in depth and dozens of nanometers in diameter. The correlation coefficient between the areas of nanobubbles and that of the corresponding hollows reached 0.88―0.94, which strongly supported the assumption that the hollows were indeed caused by the nanobubbles. Moreover, the BSA molecules formed rings around the nanobubbles, suggesting the preference of BSA adsorption onto the contact line at water/HOPG interface.展开更多
文摘首先归纳了多学科交叉实验室--致远创新研究中心的特点及管理难点;随后结合EHS(Environment,Health and Safety)管理体系,深入分析了多学科交叉实验室的安全管理机制及其特色,重点介绍线上、线下相结合的实验室安全教育与管理体系,最后总结了实验室安全管理的实践经验,以期为我国新兴多学科交叉实验室的安全管理工作提供借鉴。
基金supported by the National Basic Research Program of China (2006CB932505 and 2007CB936004)
文摘Enhancement of the SNR (signal to noise ratio) in single-molecule imaging is significantly important for improving image resolu-tion and distinguishing the fine structures of single molecules at a higher precision level.Image processing techniques have dem-onstrated the remarkable capability to improve the SNR and the resolution level by breaking through some inherent limitations unresolved by instrument hardware optimization.In this paper, we focus on single-biomolecule imaging using atomic force mi-croscopy (AFM), a unique method in separated single-biomolecule imaging, and there was few suitable image processing tech-niques reported to increase the SNR of a single molecule’s AFM image.With the typical samples of separately dispersed DNA molecules, we replaced the classified averaging method, which is usually used when the molecules’ structure can be easily and repeatedly prepared, with the time averaging method to improve the SNR in a single molecule’s AFM imaging.Combining the time averaging technique with the image alignment method for the series of time-lapse AFM images of a single DNA molecule, we were able to improve the image’s SNR and recover some buried signals from the background noises.Furthermore, the fine structures of single molecules can potentially be further resolved if other image processing techniques are applied collaboratively with some newly developed imaging methods like Vibrating Mode Scanning Polarization Force Microscopy (VSPFM), and such combination will also be advantageous for the manipulation of single-biomolecules by AFM.In addition, the proposed algorithms for the estimations of drift, distortion and SNR are applicable for quantitative status characterization of AFM imaging.
基金National Natural Science Foundation of China (Grant No. 20403010)
文摘Nanobubbles have been proven existent at the liquid/solid interface, and become a focus of research on varied interfacial processes. In the present work, by observing in situ with atomic force microscope (AFM), we found that nanobubbles could influence the adsorption process of bovine serum albumin (BSA) on hydrophobic surface of highly ordered pyrolytic graphite (HOPG). BSA could adsorb evenly, and coexist with nanobubbles at water/HOPG interface. After removing nanobubbles by injecting ethanol, some hollows were found in the BSA layers at the same positions of nanobubbles existing previously. These hollows were about 8 nm in depth and dozens of nanometers in diameter. The correlation coefficient between the areas of nanobubbles and that of the corresponding hollows reached 0.88―0.94, which strongly supported the assumption that the hollows were indeed caused by the nanobubbles. Moreover, the BSA molecules formed rings around the nanobubbles, suggesting the preference of BSA adsorption onto the contact line at water/HOPG interface.
