[目的]研制止血、止痛、抗菌多功能一体的应用于开放战创伤的新型敷料并评价其疗效.[方法]将壳聚糖、醋酸、聚乙烯醇、蒸馏水溶液、氨甲环酸、罗哌卡因以及纳米银按照一定的比例和浓度配置溶液并通过静电纺丝制备纤维膜.通过紫外线分光...[目的]研制止血、止痛、抗菌多功能一体的应用于开放战创伤的新型敷料并评价其疗效.[方法]将壳聚糖、醋酸、聚乙烯醇、蒸馏水溶液、氨甲环酸、罗哌卡因以及纳米银按照一定的比例和浓度配置溶液并通过静电纺丝制备纤维膜.通过紫外线分光光度计测定此三种药物的体外累积释放度.16只大耳白兔制作开放伤兔模型,随机分为新型敷料组8只,和传统敷料组8只,用相应敷料覆盖创面.评估出血量、兔疼痛评分、C反应蛋白(CRP)、创口组织病理切片中5个多形核粒细胞(polymorphonuclear granulocytes,PMN)最多的高倍镜视野中的平均值、创口完全愈合需要的时间.[结果]此三种药物累积释放量达到50%时需要大约12h,而20h时达到释放平衡;新型敷料组失血量、术后第1d平均疼痛指数、术后第3 d CRP、PMN均数及伤口愈合时间显著低于传统敷料组,差异有统计学意义(P<0.05),而术后第7d疼痛指数、术后第1 d CRP及术后第1 d PMN均数两组差异无统计学意义(P>0.05).[结论]载氨甲环酸、罗哌卡因及纳米银的壳聚糖/聚乙烯醇静电纺丝纤维缓释敷料可稳定缓释药物,并对兔战创伤模型具有止血、止痛、抗感染及促进伤口愈合的作用.展开更多
Background Natural disasters have been frequent in recent years. Effective treatment of patients with cardiovascular disease following natural disasters is an unsolved problem. We aimed to develop a novel miniature mo...Background Natural disasters have been frequent in recent years. Effective treatment of patients with cardiovascular disease following natural disasters is an unsolved problem. We aimed to develop a novel miniature mobile cardiac catheterization laboratory （Mini Mobile Cath Lab） to provide emergency interventional services for patients with critical cardiovascular disease following natural disasters. A feasibility study was performed by testing the Mini Mobile Cath Lab on dogs with ST-elevation myocardial infarction （STEMI） model in a hypothetical natural-disaster-stricken area. Methods The Mini Mobile Cath Lab was transported to the hypothetical natural-disaster-stricken area by truck. Coronary angiography and primary percutaneous coronary intervention （PCI） were performed on six dogs with STEMI model. The transportation and transformation of the Mini Mobile Cath Lab were monitored and its functioning was evaluated through the results of animal experiments. Results The Mini Mobile Cath Lab could be transpdrted by truck at an average speed of 80 km/h on mountain roads during daytime in the winter, under conditions of light snow （-15℃ to -20℃/-68°F to -59°F）. The average time required to prepare the Mini Mobile Cath Lab after transportation, in a wetland area, was 30 minutes. Coronary angiography, and primary PCI were performed successfully. Conclusion This preliminary feasibility study of the use of the Mini Mobile Cath Lab for emergency interventional treatment of dogs with STEMI indicated that it may perform well in the rescue of critical cardiovascular disease following natural disasters.展开更多
文摘[目的]研制止血、止痛、抗菌多功能一体的应用于开放战创伤的新型敷料并评价其疗效.[方法]将壳聚糖、醋酸、聚乙烯醇、蒸馏水溶液、氨甲环酸、罗哌卡因以及纳米银按照一定的比例和浓度配置溶液并通过静电纺丝制备纤维膜.通过紫外线分光光度计测定此三种药物的体外累积释放度.16只大耳白兔制作开放伤兔模型,随机分为新型敷料组8只,和传统敷料组8只,用相应敷料覆盖创面.评估出血量、兔疼痛评分、C反应蛋白(CRP)、创口组织病理切片中5个多形核粒细胞(polymorphonuclear granulocytes,PMN)最多的高倍镜视野中的平均值、创口完全愈合需要的时间.[结果]此三种药物累积释放量达到50%时需要大约12h,而20h时达到释放平衡;新型敷料组失血量、术后第1d平均疼痛指数、术后第3 d CRP、PMN均数及伤口愈合时间显著低于传统敷料组,差异有统计学意义(P<0.05),而术后第7d疼痛指数、术后第1 d CRP及术后第1 d PMN均数两组差异无统计学意义(P>0.05).[结论]载氨甲环酸、罗哌卡因及纳米银的壳聚糖/聚乙烯醇静电纺丝纤维缓释敷料可稳定缓释药物,并对兔战创伤模型具有止血、止痛、抗感染及促进伤口愈合的作用.
文摘Background Natural disasters have been frequent in recent years. Effective treatment of patients with cardiovascular disease following natural disasters is an unsolved problem. We aimed to develop a novel miniature mobile cardiac catheterization laboratory （Mini Mobile Cath Lab） to provide emergency interventional services for patients with critical cardiovascular disease following natural disasters. A feasibility study was performed by testing the Mini Mobile Cath Lab on dogs with ST-elevation myocardial infarction （STEMI） model in a hypothetical natural-disaster-stricken area. Methods The Mini Mobile Cath Lab was transported to the hypothetical natural-disaster-stricken area by truck. Coronary angiography and primary percutaneous coronary intervention （PCI） were performed on six dogs with STEMI model. The transportation and transformation of the Mini Mobile Cath Lab were monitored and its functioning was evaluated through the results of animal experiments. Results The Mini Mobile Cath Lab could be transpdrted by truck at an average speed of 80 km/h on mountain roads during daytime in the winter, under conditions of light snow （-15℃ to -20℃/-68°F to -59°F）. The average time required to prepare the Mini Mobile Cath Lab after transportation, in a wetland area, was 30 minutes. Coronary angiography, and primary PCI were performed successfully. Conclusion This preliminary feasibility study of the use of the Mini Mobile Cath Lab for emergency interventional treatment of dogs with STEMI indicated that it may perform well in the rescue of critical cardiovascular disease following natural disasters.
