目的探讨密歇根大学手概况问卷调查表(Michigan Hand Outcomes Questionnaire,MHQ)在手外科的应用效果并,为临床上使用该患者主观自评量表提供参考。方法查阅近年国内外MHQ应用的相关文献,总结分析MHQ在手外伤、周围神经卡压性疾病和关...目的探讨密歇根大学手概况问卷调查表(Michigan Hand Outcomes Questionnaire,MHQ)在手外科的应用效果并,为临床上使用该患者主观自评量表提供参考。方法查阅近年国内外MHQ应用的相关文献,总结分析MHQ在手外伤、周围神经卡压性疾病和关节炎等方面的应用。结果文献显示MHQ具有较好的信度、效度及敏感度,不但可以对手功能进行整体评估,而且可以利用某一子量表对某一方面进行单独评估。结论目前MHQ在国外已成为手外科常用的主观自评量表之一,但国内应用较少,相信随着对患者主观自评的重视,其研究和应用会越来越多。展开更多
为确定患病半滑舌鳎的病原,从其体内分离到3株革兰氏阴性菌,将其分别命名为ST-1、ST-3和ST-6,进行细菌理化特性、药敏试验、16S rDNA测序以及组织病理学观察等方面的研究,并建立了外膜蛋白(OMP)基因的SYBR Green I荧光定量PCR检测方法...为确定患病半滑舌鳎的病原,从其体内分离到3株革兰氏阴性菌,将其分别命名为ST-1、ST-3和ST-6,进行细菌理化特性、药敏试验、16S rDNA测序以及组织病理学观察等方面的研究,并建立了外膜蛋白(OMP)基因的SYBR Green I荧光定量PCR检测方法。结果显示,3株分离菌株的理化特性符合创伤弧菌特征,16S rDNA与创伤弧菌标准菌株ATCC29307序列相似性达99%以上,系统发育树分析显示,3株分离株与创伤弧菌自然聚为一支,最终鉴定为创伤弧菌;药敏试验显示,3株菌对头孢克肟、头孢哌酮、链霉素、亚胺培南和氟苯尼考等药物高度敏感。人工回归感染试验表明,分离株具有致病性,且半滑舌鳎、斑马鱼的临床症状与自然患病鱼症状相似;组织病理学观察显示,鳃丝上皮细胞增生,鳃小片肿胀黏连、颗粒细胞和黏液细胞增多,肝脏中央静脉淤血,肾脏出血、坏死,肠道黏膜固有层萎缩、黏液细胞增多等。进一步建立OMP基因的荧光定量PCR方法,结果显示,标准曲线的相关系数为0.995,检测下限为1.88×10~2 CFU/mL。以上结果可为半滑舌鳎弧菌病的致病机理、分子诊断和防治提供科学参考。展开更多
Intracytoplasmic infection with a prokaryotic mycoplasma-like organism (MLO) were characterized in the scallop Argopecten irradians, and the first report of such infection in this scallop was represented. Ultrastruc...Intracytoplasmic infection with a prokaryotic mycoplasma-like organism (MLO) were characterized in the scallop Argopecten irradians, and the first report of such infection in this scallop was represented. Ultrastructurally these microorganisms are usually pleomorphic and variable in morphology and size, and appear in several cell types such as small spherical body, rod-shaped bodies and longer filament-shaped body. They lack a cell wall. These observations revealed that these types of the prokaryote showed some characteristics of a mycoplasma-like organism (MLO). MLO reproduced in two ways: binary fission and budding. The results of isolation and purification showed that a large number of MLOs existed in the tissues of diseased scallops. The results of experimental infection revealed that the MLO is pathogenic to the scallop Argopecten irradians.展开更多
文摘目的探讨密歇根大学手概况问卷调查表(Michigan Hand Outcomes Questionnaire,MHQ)在手外科的应用效果并,为临床上使用该患者主观自评量表提供参考。方法查阅近年国内外MHQ应用的相关文献,总结分析MHQ在手外伤、周围神经卡压性疾病和关节炎等方面的应用。结果文献显示MHQ具有较好的信度、效度及敏感度,不但可以对手功能进行整体评估,而且可以利用某一子量表对某一方面进行单独评估。结论目前MHQ在国外已成为手外科常用的主观自评量表之一,但国内应用较少,相信随着对患者主观自评的重视,其研究和应用会越来越多。
基金This study was supported by the National Natural Science Foundation of China under contract No.30170741.
文摘Intracytoplasmic infection with a prokaryotic mycoplasma-like organism (MLO) were characterized in the scallop Argopecten irradians, and the first report of such infection in this scallop was represented. Ultrastructurally these microorganisms are usually pleomorphic and variable in morphology and size, and appear in several cell types such as small spherical body, rod-shaped bodies and longer filament-shaped body. They lack a cell wall. These observations revealed that these types of the prokaryote showed some characteristics of a mycoplasma-like organism (MLO). MLO reproduced in two ways: binary fission and budding. The results of isolation and purification showed that a large number of MLOs existed in the tissues of diseased scallops. The results of experimental infection revealed that the MLO is pathogenic to the scallop Argopecten irradians.