Neuropeptides are widely distributed in vertebrates and invertebrates,regulating a variety of physiological activities in the organisms,such as metabolism,feeding and reproduction.In this study,to explore the function...Neuropeptides are widely distributed in vertebrates and invertebrates,regulating a variety of physiological activities in the organisms,such as metabolism,feeding and reproduction.In this study,to explore the function of neuropeptide LFRFamide in Sepia pharaonis,the full-length cDNA of LFRFamide-like gene(named SpLFRFL,MG869822.1)was identified with rapid amplification of cDNA ends(RACE)method.The sequence of SpLFRFL was 860 bp in length and encoded 188 amino acids containing 4 different mature peptides:1 copy of PHTPFRFamide,NSLFRFamide,TIFRFamide,and 3 copies of GNLFRFamide.Multiple alignment and phylogenetic analysis results showed that SpLFRFL shared high identity with LFRFamides of Sepia officinalis and Sepiella japonica and had the closest relationship with them.Through quantitative Real-time PCR(qRT-PCR),it was found that the SpLFRFL gene was highly expressed in the optic lobe and brain at three different stages during gonad development in both genders.Moreover,the four mature peptides at a concentration of 0.01μmol L^(−1) could inhibit the protein synthesis in the Chinese hamster ovary cell strain-K1(CHOK1)induced by SpGnRH.These data suggest that SpLFRFL might be involved in the development and reproduction of S.pharaonis.The results can contribute to future studies on neuropeptide evolution and function and benefit the cuttlefish farming.展开更多
In the formulation development of laundry gel beads,4,4-dichloro-2-hydroxydiphenyl ether(HP-100)was used as a bacteriostatic agent,and the effect of HP-100 dosage on the bacteriostatic rate of laundry gel bead samples...In the formulation development of laundry gel beads,4,4-dichloro-2-hydroxydiphenyl ether(HP-100)was used as a bacteriostatic agent,and the effect of HP-100 dosage on the bacteriostatic rate of laundry gel bead samples with different formulation systems was examined by the suspension quantification method;the long-lasting bacteriostatic performance of laundry gel bead samples added with HP-100 was investigated by the absorption method for evaluating the antimicrobial performance of textiles;the effect of HP-100 on the odor control of laundry gel bead samples was investigated by filling out surveys and questionnaires using the actual measurement method.Questionnaire was used to investigate the effect of HP-100 on odor control.The test results showed that the antibacterial rate of the two laundry gel samples against S.aureus increased with the increase of HP-100 dosage,and the antibacterial rate of Formula 1 reached 99.9%when the dosage of HP-100 was 0.3%;the antibacterial rate of cotton cloth washed with the laundry gel sample of Formula 1+0.3%HP-100 was more than 50%after 24 h,and still had the antibacterial effect;the antibacterial rate of cotton cloth washed with the sample of laundry gel with HP-100 was more than 50%after 24 h,and still had the antibacterial effect;the antibacterial effect of the sample with HP-100 was more than 50%after 24 h,and still had the antibacterial effect.The fabrics washed with HP-100 added laundry gel beads had better odor control than those treated with laundry gel beads without HP-100,and the addition of HP-100 to laundry gel beads could have antibacterial and deodorizing effects.展开更多
基金supported by the National Natural Science Foundation of China(No.31872547)the Natural Science Foundation of Zhejiang Province,China(No.LY20 C190007)+1 种基金the Open Foundation from Marine Sciences in the First-Class Subjects of Zhejiang(No.OFMS004)the Excellent Postdoctoral Program of Jiangsu Province(No.314865).
文摘Neuropeptides are widely distributed in vertebrates and invertebrates,regulating a variety of physiological activities in the organisms,such as metabolism,feeding and reproduction.In this study,to explore the function of neuropeptide LFRFamide in Sepia pharaonis,the full-length cDNA of LFRFamide-like gene(named SpLFRFL,MG869822.1)was identified with rapid amplification of cDNA ends(RACE)method.The sequence of SpLFRFL was 860 bp in length and encoded 188 amino acids containing 4 different mature peptides:1 copy of PHTPFRFamide,NSLFRFamide,TIFRFamide,and 3 copies of GNLFRFamide.Multiple alignment and phylogenetic analysis results showed that SpLFRFL shared high identity with LFRFamides of Sepia officinalis and Sepiella japonica and had the closest relationship with them.Through quantitative Real-time PCR(qRT-PCR),it was found that the SpLFRFL gene was highly expressed in the optic lobe and brain at three different stages during gonad development in both genders.Moreover,the four mature peptides at a concentration of 0.01μmol L^(−1) could inhibit the protein synthesis in the Chinese hamster ovary cell strain-K1(CHOK1)induced by SpGnRH.These data suggest that SpLFRFL might be involved in the development and reproduction of S.pharaonis.The results can contribute to future studies on neuropeptide evolution and function and benefit the cuttlefish farming.
文摘In the formulation development of laundry gel beads,4,4-dichloro-2-hydroxydiphenyl ether(HP-100)was used as a bacteriostatic agent,and the effect of HP-100 dosage on the bacteriostatic rate of laundry gel bead samples with different formulation systems was examined by the suspension quantification method;the long-lasting bacteriostatic performance of laundry gel bead samples added with HP-100 was investigated by the absorption method for evaluating the antimicrobial performance of textiles;the effect of HP-100 on the odor control of laundry gel bead samples was investigated by filling out surveys and questionnaires using the actual measurement method.Questionnaire was used to investigate the effect of HP-100 on odor control.The test results showed that the antibacterial rate of the two laundry gel samples against S.aureus increased with the increase of HP-100 dosage,and the antibacterial rate of Formula 1 reached 99.9%when the dosage of HP-100 was 0.3%;the antibacterial rate of cotton cloth washed with the laundry gel sample of Formula 1+0.3%HP-100 was more than 50%after 24 h,and still had the antibacterial effect;the antibacterial rate of cotton cloth washed with the sample of laundry gel with HP-100 was more than 50%after 24 h,and still had the antibacterial effect;the antibacterial effect of the sample with HP-100 was more than 50%after 24 h,and still had the antibacterial effect.The fabrics washed with HP-100 added laundry gel beads had better odor control than those treated with laundry gel beads without HP-100,and the addition of HP-100 to laundry gel beads could have antibacterial and deodorizing effects.
