Based on the LC-ESI/MSn technique, the four pairs of isomers of psoralen and isopsoralen, imperatorin and isoimperatorin, neohesperidin and hesperidin, naringin and narirutin were distinguished mainly by comparison of...Based on the LC-ESI/MSn technique, the four pairs of isomers of psoralen and isopsoralen, imperatorin and isoimperatorin, neohesperidin and hesperidin, naringin and narirutin were distinguished mainly by comparison of the relative abundances of their major fragment ions. Because of the effect of the hydrogen on the C8 of psoralen, the intensity of the fragment ion formed by the loss of CO2 was relatively increased. By comparing the abundance of the product ions formed by the loss of CO and 2CO, the isomers of psoralen and isopsoralen could be distinguished. This method can also be used to distinguish the isomers of imperatorin and isoimperatorin. Because of the different con- nected position between glucose and rhamnose, in the mass spectrum of neohesperidin, the relative abundances of ions at m/z 489 and 343 generated by the ring-cross cleavage of glycan were more than 10%, while in the mass spec- trum of hesperidin, they were all less than 1%. This approach can also be used to distinguish the isomers ofnaringin and narirutin. Although the fragmentation pathways were similar, according to the tiny difference of structure, the change of relative intensity of main fragment ions could be observed and it could be used to distinguish some isomers more accurately and quickly.展开更多
目的:从代谢组学角度研究塞络通胶囊治疗急性多发性脑梗死大鼠模型的作用机制。方法:24只SD大鼠随机分为假手术组、模型组和塞络通组(33 mg·kg^-1),通过大鼠颈内动脉注射荧光微球的方法建立急性多发性脑梗死大鼠模型。手术成功后,...目的:从代谢组学角度研究塞络通胶囊治疗急性多发性脑梗死大鼠模型的作用机制。方法:24只SD大鼠随机分为假手术组、模型组和塞络通组(33 mg·kg^-1),通过大鼠颈内动脉注射荧光微球的方法建立急性多发性脑梗死大鼠模型。手术成功后,塞络通组大鼠通过十二指肠注射给药,给药体积2 m L·kg^-1。基于UPLC-Q-TOF-MS分析各组大鼠脑组织中内源性代谢物,应用主成分分析(PCA)和偏最小二乘法-判别分析(PLS-DA)进行模式识别及生物标志物的鉴定。结果:通过模式识别发现假手术组与模型组的代谢轮廓有明显差异,并鉴定了10个与急性脑缺血相关的生物标志物。与假手术组相比,模型组中N-乙酰天门冬氨酸(NAA),延胡索酸,谷胱甘肽,脱氢抗坏血酸,天门冬氨酸以及S-腺苷同型半胱氨酸含量下降,精氨酸、瓜氨酸、酵母氨酸、乙内酰脲-5-丙酸含量升高;而塞络通胶囊则可使上述10个异常变化的生物标志物水平显著回调。结论:塞络通胶囊主要调节的代谢途径有NAA代谢、精氨酸代谢、能量代谢及氧化应激等。展开更多
基金Supported by the National Natural Science Foundation of China(Nos.30670212 and 30870262)
文摘Based on the LC-ESI/MSn technique, the four pairs of isomers of psoralen and isopsoralen, imperatorin and isoimperatorin, neohesperidin and hesperidin, naringin and narirutin were distinguished mainly by comparison of the relative abundances of their major fragment ions. Because of the effect of the hydrogen on the C8 of psoralen, the intensity of the fragment ion formed by the loss of CO2 was relatively increased. By comparing the abundance of the product ions formed by the loss of CO and 2CO, the isomers of psoralen and isopsoralen could be distinguished. This method can also be used to distinguish the isomers of imperatorin and isoimperatorin. Because of the different con- nected position between glucose and rhamnose, in the mass spectrum of neohesperidin, the relative abundances of ions at m/z 489 and 343 generated by the ring-cross cleavage of glycan were more than 10%, while in the mass spec- trum of hesperidin, they were all less than 1%. This approach can also be used to distinguish the isomers ofnaringin and narirutin. Although the fragmentation pathways were similar, according to the tiny difference of structure, the change of relative intensity of main fragment ions could be observed and it could be used to distinguish some isomers more accurately and quickly.
文摘目的:从代谢组学角度研究塞络通胶囊治疗急性多发性脑梗死大鼠模型的作用机制。方法:24只SD大鼠随机分为假手术组、模型组和塞络通组(33 mg·kg^-1),通过大鼠颈内动脉注射荧光微球的方法建立急性多发性脑梗死大鼠模型。手术成功后,塞络通组大鼠通过十二指肠注射给药,给药体积2 m L·kg^-1。基于UPLC-Q-TOF-MS分析各组大鼠脑组织中内源性代谢物,应用主成分分析(PCA)和偏最小二乘法-判别分析(PLS-DA)进行模式识别及生物标志物的鉴定。结果:通过模式识别发现假手术组与模型组的代谢轮廓有明显差异,并鉴定了10个与急性脑缺血相关的生物标志物。与假手术组相比,模型组中N-乙酰天门冬氨酸(NAA),延胡索酸,谷胱甘肽,脱氢抗坏血酸,天门冬氨酸以及S-腺苷同型半胱氨酸含量下降,精氨酸、瓜氨酸、酵母氨酸、乙内酰脲-5-丙酸含量升高;而塞络通胶囊则可使上述10个异常变化的生物标志物水平显著回调。结论:塞络通胶囊主要调节的代谢途径有NAA代谢、精氨酸代谢、能量代谢及氧化应激等。
