目的分析近70年来中医药疗法在玫瑰痤疮(Rosacea)领域的发展状况、研究热点,探索该领域的动态前沿。方法以“玫瑰痤疮”“酒渣鼻”为研究主题,检索中国知网(China National Knowledge Infrastructure,CNKI)建库至2022年12月31日相关文献...目的分析近70年来中医药疗法在玫瑰痤疮(Rosacea)领域的发展状况、研究热点,探索该领域的动态前沿。方法以“玫瑰痤疮”“酒渣鼻”为研究主题,检索中国知网(China National Knowledge Infrastructure,CNKI)建库至2022年12月31日相关文献,应用CiteSpace 6.1.R6进行作者、机构、关键词的共现分析,并绘制相关可视化图谱进行分析。结果中医药治疗玫瑰痤疮相关文献发文量自2016年起逐年上升,共获得文献243篇;2016-2022年,为快速增长阶段,近7年内平均发文量更是增长到17.14篇;核心作者3位,发现该研究领域作者间的分散程度较大、合作较少,有待改善;研究机构主要由成都中医药大学、黑龙江中医药大学、辽宁中医药大学组成,各核心作者及大学机构之间合作不密切;热点关键词为酒渣鼻、玫瑰痤疮、临床观察、中药、鼻赘期、中药面膜、中医、针刺、临床疗效、火针疗法、名医经验、酒渣鼻方、肺胃热盛、刺络拔罐、数据挖掘等,研究热点集中在疾病观察、中医药不同治法及辨证探索上。结论中医药治疗玫瑰痤疮切实可行,效果显著,但其发病机制尚不清楚,还需各团队机构加强交流与合作,开展更多高质量相关研究,促进中医药疗法的治疗特色与优势持续发展。展开更多
To separate the proteins related to pigment synthesis in green colored fiber （GCF）, we performed a comparative proteomic analysis to identify the differentially expressed proteins between green cotton fiber and a wh...To separate the proteins related to pigment synthesis in green colored fiber （GCF）, we performed a comparative proteomic analysis to identify the differentially expressed proteins between green cotton fiber and a white near-isogenic line （NIL）. One differential spot identified as phenylocumaran benzylic ether redutase-like protein （PCBER） was expressed only in GCF, but was not found in white colored fiber （WCF） at any time points. Since PCBER was a key enzyme in lignans biosynthesis, total lignans were extracted from GCF and WCF and their content was determined by using a chromotropic acid spectrophotometric method. The results showed that total lignans content in GCF was significantly higher than that in WCF. The qPCR analysis for two PLR genes associated with lignans biosynthesis showed that the expression level of two genes was much higher in GCF than that in WCF at 24 and 27 days post anthesis （DPA）, which may be responsible for the higher lignans content in GCF. Our study suggested that PCBER and lignans may be responsible for the color difference between GCF and WCF. Additionally, p-dimethylaminocinnamaldehyde （DMACA） staining demonstrated that the pigment in GCF was not proanthocyanidins, and was different from that in brown colored fiber （BCF）. This study provided new clues for uncovering the molecular mechanisms related to pigment biosynthesis in GCF.展开更多
文摘目的分析近70年来中医药疗法在玫瑰痤疮(Rosacea)领域的发展状况、研究热点,探索该领域的动态前沿。方法以“玫瑰痤疮”“酒渣鼻”为研究主题,检索中国知网(China National Knowledge Infrastructure,CNKI)建库至2022年12月31日相关文献,应用CiteSpace 6.1.R6进行作者、机构、关键词的共现分析,并绘制相关可视化图谱进行分析。结果中医药治疗玫瑰痤疮相关文献发文量自2016年起逐年上升,共获得文献243篇;2016-2022年,为快速增长阶段,近7年内平均发文量更是增长到17.14篇;核心作者3位,发现该研究领域作者间的分散程度较大、合作较少,有待改善;研究机构主要由成都中医药大学、黑龙江中医药大学、辽宁中医药大学组成,各核心作者及大学机构之间合作不密切;热点关键词为酒渣鼻、玫瑰痤疮、临床观察、中药、鼻赘期、中药面膜、中医、针刺、临床疗效、火针疗法、名医经验、酒渣鼻方、肺胃热盛、刺络拔罐、数据挖掘等,研究热点集中在疾病观察、中医药不同治法及辨证探索上。结论中医药治疗玫瑰痤疮切实可行,效果显著,但其发病机制尚不清楚,还需各团队机构加强交流与合作,开展更多高质量相关研究,促进中医药疗法的治疗特色与优势持续发展。
基金supported by the National Natural Science Foundation of China (31460360)the National Key Research and Development Program,China (2016YFD0101900)the Foundation Research Funds for Advanced Talents of Shihezi University,China (RCZX201316)
文摘To separate the proteins related to pigment synthesis in green colored fiber （GCF）, we performed a comparative proteomic analysis to identify the differentially expressed proteins between green cotton fiber and a white near-isogenic line （NIL）. One differential spot identified as phenylocumaran benzylic ether redutase-like protein （PCBER） was expressed only in GCF, but was not found in white colored fiber （WCF） at any time points. Since PCBER was a key enzyme in lignans biosynthesis, total lignans were extracted from GCF and WCF and their content was determined by using a chromotropic acid spectrophotometric method. The results showed that total lignans content in GCF was significantly higher than that in WCF. The qPCR analysis for two PLR genes associated with lignans biosynthesis showed that the expression level of two genes was much higher in GCF than that in WCF at 24 and 27 days post anthesis （DPA）, which may be responsible for the higher lignans content in GCF. Our study suggested that PCBER and lignans may be responsible for the color difference between GCF and WCF. Additionally, p-dimethylaminocinnamaldehyde （DMACA） staining demonstrated that the pigment in GCF was not proanthocyanidins, and was different from that in brown colored fiber （BCF）. This study provided new clues for uncovering the molecular mechanisms related to pigment biosynthesis in GCF.
