Objective:To investigate the therapeutic efficacy and underlying mechanisms of LiZhong Tang in the context of non-alcoholic fatty liver disease(NAFLD).Methods:High-fat feed was used to induce NAFLD in rats,Blood and l...Objective:To investigate the therapeutic efficacy and underlying mechanisms of LiZhong Tang in the context of non-alcoholic fatty liver disease(NAFLD).Methods:High-fat feed was used to induce NAFLD in rats,Blood and liver samples were collected to facilitate a comparative analysis of rat body mass and liver wet weight and calculate the liver index.Liver pathology was observed,while serum transaminase and blood lipid levels were measured.The protein expression levels of PINK1,Parkin,and LC-3II in rat liver were detected using Western Blot analysis.Results:Compared with the control group,the NAFLD rats exhibited a significant increase in body weight,liver wet weight,liver index,transaminase levels,and blood lipid levels.The expression levels of PINK1,Parkin,and LC3-II protein were significantly decreased(P<0.01).Following intervention with Lizhong Tang,rats in each herbal treatment group displayed a decrease in body weight,liver wet weight,liver index,se-rum transaminase,and blood lipid levels.The expression levels of PINK1,Parkin,and LC-3II rebounded(P<0.05),with the high-dose group demonstrating the most pronounced effects(P<0.01).Histopathological examination of liver tissue revealed that rats in the model group displayed disrupted hepatic lobule structure,swollen hepatocytes,disordered arrangement,and a multi-tude of varying-sized lipid vacuoles within the cytoplasm.Conversely,rats treated with different doses of the herbal remedy exhibited improvements in liver tissue pathology,with the high-dose group showing the most notable enhancement.Conclusion:Lizhong Tang can improve NAFLD disease by regulating mitochondrial autophagy.展开更多
SATB1在乳腺癌的侵袭转移中起到决定性作用,抑制SATB1的表达能够有效地控制乳腺癌的转移。该实验从茯苓中分离纯化得到均一多糖并制备硫酸化衍生物,筛选具有抑制SATB1表达的多糖成分。茯苓经醇提去除小分子成分,水提醇沉后得茯苓粗多糖P...SATB1在乳腺癌的侵袭转移中起到决定性作用,抑制SATB1的表达能够有效地控制乳腺癌的转移。该实验从茯苓中分离纯化得到均一多糖并制备硫酸化衍生物,筛选具有抑制SATB1表达的多糖成分。茯苓经醇提去除小分子成分,水提醇沉后得茯苓粗多糖PPS,PPS经DEAE Sepharose Fast Flow阴离子交换树脂分离和Superdex-75系列凝胶纯化后得PPSW-1,并对PPSW-1进行结构分析、硫酸衍生化和抑制人乳腺癌MDA-MB-231细胞迁移作用考察。结果显示从茯苓中得到一种具有抑制人乳腺癌MDA-MB-231细胞迁移作用的均一多糖PPSW-1,相对分子质量为3. 06×104,结构为1,3连接α-D-半乳聚糖为主链,1,6连接α-D-半乳聚糖为支链的多糖,PPSW-1表现出较强的抑制细胞迁移的作用,经硫酸衍生化后产物Sul-W-1活性较PPSW-1并未有明显的增强,但水溶性有明显增加,并且发现茯苓多糖及其硫酸化衍生物能抑制SATB1的表达。该研究从茯苓中成功分离到具有抑制人乳腺癌MDA-MB-231细胞迁移作用的均一多糖,并经过硫酸衍生化得到活性不变溶解性更好的多糖,该发现为茯苓多糖及其衍生物的抗乳腺癌研究提供依据。展开更多
基金Youth Fund of Guangxi Natural Science Foundat ion (No.2020GXNSFBA297133)Guangxi Traditional Chinese Medicine Administration Science and Technology Project (No.GZSY20-60)+1 种基金Natural Science Youth Fund of Guangxi University of Traditional Chinese Medicine (No.2020QN020)Guangxi Qihuang Scholar Training Project.
文摘Objective:To investigate the therapeutic efficacy and underlying mechanisms of LiZhong Tang in the context of non-alcoholic fatty liver disease(NAFLD).Methods:High-fat feed was used to induce NAFLD in rats,Blood and liver samples were collected to facilitate a comparative analysis of rat body mass and liver wet weight and calculate the liver index.Liver pathology was observed,while serum transaminase and blood lipid levels were measured.The protein expression levels of PINK1,Parkin,and LC-3II in rat liver were detected using Western Blot analysis.Results:Compared with the control group,the NAFLD rats exhibited a significant increase in body weight,liver wet weight,liver index,transaminase levels,and blood lipid levels.The expression levels of PINK1,Parkin,and LC3-II protein were significantly decreased(P<0.01).Following intervention with Lizhong Tang,rats in each herbal treatment group displayed a decrease in body weight,liver wet weight,liver index,se-rum transaminase,and blood lipid levels.The expression levels of PINK1,Parkin,and LC-3II rebounded(P<0.05),with the high-dose group demonstrating the most pronounced effects(P<0.01).Histopathological examination of liver tissue revealed that rats in the model group displayed disrupted hepatic lobule structure,swollen hepatocytes,disordered arrangement,and a multi-tude of varying-sized lipid vacuoles within the cytoplasm.Conversely,rats treated with different doses of the herbal remedy exhibited improvements in liver tissue pathology,with the high-dose group showing the most notable enhancement.Conclusion:Lizhong Tang can improve NAFLD disease by regulating mitochondrial autophagy.
文摘SATB1在乳腺癌的侵袭转移中起到决定性作用,抑制SATB1的表达能够有效地控制乳腺癌的转移。该实验从茯苓中分离纯化得到均一多糖并制备硫酸化衍生物,筛选具有抑制SATB1表达的多糖成分。茯苓经醇提去除小分子成分,水提醇沉后得茯苓粗多糖PPS,PPS经DEAE Sepharose Fast Flow阴离子交换树脂分离和Superdex-75系列凝胶纯化后得PPSW-1,并对PPSW-1进行结构分析、硫酸衍生化和抑制人乳腺癌MDA-MB-231细胞迁移作用考察。结果显示从茯苓中得到一种具有抑制人乳腺癌MDA-MB-231细胞迁移作用的均一多糖PPSW-1,相对分子质量为3. 06×104,结构为1,3连接α-D-半乳聚糖为主链,1,6连接α-D-半乳聚糖为支链的多糖,PPSW-1表现出较强的抑制细胞迁移的作用,经硫酸衍生化后产物Sul-W-1活性较PPSW-1并未有明显的增强,但水溶性有明显增加,并且发现茯苓多糖及其硫酸化衍生物能抑制SATB1的表达。该研究从茯苓中成功分离到具有抑制人乳腺癌MDA-MB-231细胞迁移作用的均一多糖,并经过硫酸衍生化得到活性不变溶解性更好的多糖,该发现为茯苓多糖及其衍生物的抗乳腺癌研究提供依据。
