IM To investigate the agerelated alterations of cytoskeleton system in liver Kupffer cell and their relation to the changed phagocytic function.METHODS The phagocytic function of Kupffer cells from rats of various a...IM To investigate the agerelated alterations of cytoskeleton system in liver Kupffer cell and their relation to the changed phagocytic function.METHODS The phagocytic function of Kupffer cells from rats of various ages (6mo, 12mo, 18mo and 24mo) were quantitatively evaluated by phagocytosis of polystyrene beads. The actin distribution and measurement of Kupffer cell were determined by a phalloidinTRITC method; and the myosin and vimentin distribution and measurement with indirect immunochemical staining.RESULTS Aging resulted in significant alterations of actin, myosin and vimentin distributions and reductions in Kupffer cell; the 3 cytoskeleton components of 24moold Kupffer cell were significantly decreased to 680%, 849% and 755%, respectively of these of 6moold Kupffer cell(P<001,001 and 001). And these decreases had significant positive relations with the damaged phagocytosis of the aged Kupffer cell. γ values were 096(P<005), 099(P<001) and 095 (P<005) respectively.CONCLUSION The cytoskeleton system of the aged Kupffer cell presents an evident state of senescence, which may be an important mechanism of decreased phagocytosis of the aged Kupffer cell..展开更多
Radiofrequency ablation （RFA）, especially percutaneous RFA （PRFA）, is a novel technique for the treatment of hepatocellular carcinoma （HCC） that is becoming increasingly more popular because of its feasibility, ...Radiofrequency ablation （RFA）, especially percutaneous RFA （PRFA）, is a novel technique for the treatment of hepatocellular carcinoma （HCC） that is becoming increasingly more popular because of its feasibility, effectiveness, repeatability, and safety.1 The complications of PRFA mainly include pneumothorax, hemopneumothorax, visceral organ perforation, liver abscess, bile duct injury,展开更多
Sinistral portal hypertension （SPH） is usually caused by pancreatic pathology and is characterized bysplenic vein thrombosis with or without portal vein thrombosis.1 The increased pressure caused by splenic vein occ...Sinistral portal hypertension （SPH） is usually caused by pancreatic pathology and is characterized bysplenic vein thrombosis with or without portal vein thrombosis.1 The increased pressure caused by splenic vein occlusion is transmitted via the short gastric and gastroepiploic veins to the portal system. The reversal of blood flow in the left gastric vein results in gastric varices. In patiems with SPH, especially those with occlusion of both the splenic and portal veins, the main or even the only pathway of splenic venous return to the portal vein is via the gastric varices, and these patiems require special management during gastric surgery.展开更多
文摘IM To investigate the agerelated alterations of cytoskeleton system in liver Kupffer cell and their relation to the changed phagocytic function.METHODS The phagocytic function of Kupffer cells from rats of various ages (6mo, 12mo, 18mo and 24mo) were quantitatively evaluated by phagocytosis of polystyrene beads. The actin distribution and measurement of Kupffer cell were determined by a phalloidinTRITC method; and the myosin and vimentin distribution and measurement with indirect immunochemical staining.RESULTS Aging resulted in significant alterations of actin, myosin and vimentin distributions and reductions in Kupffer cell; the 3 cytoskeleton components of 24moold Kupffer cell were significantly decreased to 680%, 849% and 755%, respectively of these of 6moold Kupffer cell(P<001,001 and 001). And these decreases had significant positive relations with the damaged phagocytosis of the aged Kupffer cell. γ values were 096(P<005), 099(P<001) and 095 (P<005) respectively.CONCLUSION The cytoskeleton system of the aged Kupffer cell presents an evident state of senescence, which may be an important mechanism of decreased phagocytosis of the aged Kupffer cell..
基金This research was supported by grants from the Natural Science Foundation of China （No. 30872490） and Dr. WU Jie-ping Medical Foundation （No. 320675007131）.
文摘Radiofrequency ablation （RFA）, especially percutaneous RFA （PRFA）, is a novel technique for the treatment of hepatocellular carcinoma （HCC） that is becoming increasingly more popular because of its feasibility, effectiveness, repeatability, and safety.1 The complications of PRFA mainly include pneumothorax, hemopneumothorax, visceral organ perforation, liver abscess, bile duct injury,
文摘Sinistral portal hypertension （SPH） is usually caused by pancreatic pathology and is characterized bysplenic vein thrombosis with or without portal vein thrombosis.1 The increased pressure caused by splenic vein occlusion is transmitted via the short gastric and gastroepiploic veins to the portal system. The reversal of blood flow in the left gastric vein results in gastric varices. In patiems with SPH, especially those with occlusion of both the splenic and portal veins, the main or even the only pathway of splenic venous return to the portal vein is via the gastric varices, and these patiems require special management during gastric surgery.
