TODI和MDI并用对浇注型聚氨酯弹性体性能的影响

以聚四氢呋喃(PTMG)为聚合物多元醇,TODI和MDI并用为异氰酸酯,添加一定量的扩链剂,采用预聚法制备了一系列浇注型聚氨酯弹性体;研究了TODI/MDI不同并用比、扩链剂种类以及扩链系数对弹性体工艺性能和力学性能的影响。结果表明,随着TODI用量的增加,可操作时间延长,弹性体的耐热性能提高;当采用PTMG 1000为聚合物多元醇,TODI/MDI并用比为60/40,1,4-BD为扩链剂,且扩链系数为0.95时,聚氨酯弹性体的综合性能最佳。

当归鸡血藤汤对再生障碍性贫血模型大鼠骨髓造血功能的影响

目的 探讨当归鸡血藤汤(DJD)对再生障碍性贫血(AA)模型大鼠骨髓造血功能的影响。方法 将SD大鼠随机分为空白组(0.9%NaCl)、模型组(0.9%NaCl)、阳性对照组(10.8 g·kg^(-1)复方阿胶浆)和低、中、高剂量实验组(3.10、6.21和12.42 g·kg^(-1)DJD)。用乙酰苯肼联合环磷酰胺建立AA大鼠模型,造模的同时给药,每天1次,连续15 d。测定大鼠体质量、血常规及骨髓中造血因子水平;观察大鼠骨髓涂片,进行骨髓有核细胞计数。结果 中、高剂量实验组和阳性对照组、模型组、空白组的血常规中白细胞数量分别为(2.44±0.46)×10^(9)、(4.26±0.38)×10^(9)、(4.57±1.43)×10^(9)、(0.96±0.18)×10^(9)和(9.83±0.87)×10^(9)·L^(-1),红细胞计数分别为(3.14±0.61)×10^(12)、(3.44±0.35)×10^(12)、(4.06±0.86)×10^(12)、(1.35±0.54)×10^(12)和(9.61±1.18)×10^(12)·L^(-1),血红蛋白数量分别为(87.83±7.25)、(108.17±8.93)、(99.50±13.81)、(22.33±7.63)和(153.92±19.42)g·L^(-1),血小板数量分别为(218.50±28.05)×10^(9)、(291.83±32.32)×10^(9)、(269.33±67.60)×10^(9)、(37.50±12.21)×10^(9)和(507.83±181.01)×10^(9)·L^(-1),骨髓中粒细胞巨噬细胞集落刺激因子水平分别为(317.08±31.86)、(359.70±36.37)、(394.86±23.41)、(198.31±14.63)和(379.39±14.95)ng·L^(-1),血管内皮细胞黏附分子1水平分别为(765.98±60.32)、(877.96±24.75)、(812.74±66.40)、(588.25±58.15)和(929.01±35.50)μg·L^(-1),白细胞介素3水平分别为(129.42±6.19)、(121.31±12.65)、(124.24±2.50)、(83.24±11.05)和(135.67±7.66)ng·L^(-1),胱天蛋白酶3水平分别为(105.49±10.07)、(115.49±6.89)、(114.36±2.97)、(150.62±16.82)和(116.10±4.68)pmol·L^(-1),骨髓损伤评分分别为(1.31±0.18)、(1.96±0.09)、(1.49±20.42)、(3.76±0.19)和(0.05±0.04)分,有核细胞计数分别为(5.79±0.47)×10^(6)、(5.12±0.10)×10^(6)、(5.56±0.25)×10^(6)、(3.20±0.28)×10^(6)和(8.49±0.56)×10^(6)·L^(-1)。空白组的上述指标与模型组比较,中、高剂量实验组及阳性对照组的上述指标与模型组比较,差异均有统计学意义(P<0.05,P<0.01)。结论 当归鸡血藤汤可能通过调控骨髓造血微环境,改善免疫功能和抑制细胞凋亡,改善AA模型大鼠骨髓造血功能。

Youjing granules ameliorate spermatogenesis in rats through regulating the prolifereation of spermatogonial stem cells

Male infertility has evolved from a common reproductive system disease to a major social issue.Youjing granule(YG)is a Chinese medicinal material used as a therapy method for tonifying the kidneys and removing dampness due to its pathogenic characteristics.YG has been shown to regulate sperm quality in clinical trials,but the underlying mechanism is not fully understood.The present study was aimed to explore the protective effects and mechanism of action of YG on male reproductive system damage caused by methyl methane sulfonate(MMS).We first established an infertility model of rats through oral administration of MMS and then treated with YG.To determine the effect of YG,spermatogenesis,microvascular density,and secretory function of Leydig cells and Sertoli cells in rats were assessed.Spermatogonial stem cells(SSCs)were co-cultured with mouse embryo fibroblast(MEF)cells as an in vitro cell model before exposure to serum containing YG.Furthermore,the proliferation and apoptosis of SSCs were measured.Results indicated that YG increased the expression of self-renewal and proliferation-related molecules such as glial cell line derived neurotrophic factor(GDNF)and fibroblast growth factor-2(FGF2),and improved the quality of sperm and the proliferation of SSCs.In conclusion,YG may protect spermatogenetic function of rats through regulating the proliferation and self-renewal of SSCs.