Research Advance of Chinese Medicine in Treating Atherosclerosis:Focus on Lipoprotein-Associated Phospholipase A2

As a serious cardiovascular disease,atherosclerosis(AS)causes chronic inflammation and oxidative stress in the body and poses a threat to human health.Lipoprotein-associated phospholipase A2(Lp-PLA2)is a member of the phospholipase A2(PLA2)family,and its elevated levels have been shown to contribute to AS.Lp-PLA2 is closely related to a variety of lipoproteins,and its role in promoting inflammatory responses and oxidative stress in AS is mainly achieved by hydrolyzing oxidized phosphatidylcholine(ox PC)to produce lysophosphatidylcholine(lyso PC).Moreover,macrophage apoptosis within plaque is promoted by localized Lp-PLA2 which also promotes plaque instability.This paper reviews those researches of Chinese medicine in treating AS via reducing Lp-PLA2 levels to guide future experimental studies and clinical applications related to AS.

基于自噬基因Beclin-1与LC3探讨丹参素对心肌缺血再灌注损伤大鼠心肌ATP5G1的影响

目的:探讨丹参素对心肌缺血再灌注损伤(IRI)大鼠的心肌保护作用,并揭示其作用机制。方法:将18只SD大鼠随机分为正常组、模型组、丹参素组。正常组持续灌注KH液90min;模型组停灌30min,复灌KH液60min;丹参素组停灌30min,复灌含有浓度为10μmol/L丹参素的KH液60min。利用离体灌流系统检测LVDP;采用ELISA法检测大鼠心肌ADP、ATP的含量以及灌流液中cTnT的酶活性;采用RT-PCR法检测自噬基因LC3、Beclin-1的表达;使用蛋白印迹法在蛋白水平测量ATP5G1的变化。结果:与正常组比较,模型组大鼠LVDP下降,ADP/ATP增加,cTnT的酶活性增强,自噬基因LC3、Beclin-1表达显著上调,ATP5G1蛋白表达显著上调(P<0.01);与模型组比较,丹参素组大鼠LVDP升高,ADP/ATP下降,cTnT的酶活性降低,自噬基因LC3、Beclin-1表达下调,ATP5G1蛋白表达下调(P<0.05)。结论:丹参素具有调节自噬、下调ATP5G1蛋白表达、促进ATP生成的作用,这可能是其改善IRI的作用机制之一。