As a matter of fact, infectious diseases hamper everyone’s life and produce a lifelong threat to everyone neutral of age, sex, lifestyle and socio-economic status. Nowadays, come into the sight of Chikungunya viral (...As a matter of fact, infectious diseases hamper everyone’s life and produce a lifelong threat to everyone neutral of age, sex, lifestyle and socio-economic status. Nowadays, come into the sight of Chikungunya viral (CHIKV) infection injured many Asian and African countries, also deliberated threat in rising countries and also low socio-economic countries. CHIKV is a positive-sense, enveloped single-stranded, RNA virus belonging to the genus Alphavirus, family Togaviridae. As the Dengue & Chikungunya viruses are spread simultaneously at the same time, so it is tough to identify them. In our resource-limited countries, swift detection of CHIKV by RT-LAMP is the simplest molecular technique in low-equipment settings without the use of any expensive decoration. Heat-treated centrifuged and uncentrifuged samples were used in this study and they showed the same result (100%). Different instruments like heat block, water bath, conventional thermal cycler & real-time thermal cycler were used to amplify the CHIKV RNA and they indicated that 100% samples were identified by all four instruments. The amplified products were visualized by turbidity test, color change by HNB, step-ladder band pattern in agarose gel electrophoresis and amplification curve in real-time thermal cycler naked eye, here the results also showed 100% samples were determined by all visualized methods. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a recent technique for amplifying RNA under limited temperature, with high tangibility, quickness and competency. To identify the CHIKV RNA Reverse transcription loop-mediated isothermal amplification was fabricated and validated, and the results were also compared with reverse transcription polymerase chain reaction (RT-PCR). The sensitivity was 95.71% and specificity was 100%, these results indicate that RT-LAMP is a feasible method for quick detection of CHIKV RNA.展开更多
Allergy is an adverse immunologic response of increasing prevalence and severity in which immunoglobulin E plays a pivotal role. Total IgE (tIgE) levels show wide variations depending on various factors, such as age, ...Allergy is an adverse immunologic response of increasing prevalence and severity in which immunoglobulin E plays a pivotal role. Total IgE (tIgE) levels show wide variations depending on various factors, such as age, sex, ethnicity, and geographic area. This study set out to quantitatively determine the serum tIgE levels in Bangladeshi population who were clinically suspected cases of allergic diseases and to find out the association of tIgE level with their age and gender. This cross-sectional descriptive exploratory study was conducted at Biomed Diagnostics Laboratory, Panthapath, Dhaka, from March 2021-September 2021. A total of 402 blood samples were collected from clinically suspected cases of allergic diseases. Quantitative detection of tIgE in serum was done by Immunoblot assay manufactured by Proteome Tech Inc. (PROTIATM Allergy-Q64S panel). Among 402 subjects, a wide variation of tIgE levels ranging from 5 to 2000 IU/ml was found but majority of the study population (30.35%) showed tIgE levels within the range of 201 - 350 IU/ml. The study population aging 1 - 10 years showed highest frequency (23.63%) in tIgE concentrations and the mean tIgE level exhibited a declining tendency with an increase in age. Depending on gender there was no difference in tIgE levels. Determination of tIgE level is imperative for screening allergic diseases. The present study enlightened the distribution of tIgE levels and suggested that among Bangladeshi subjects irrespective of gender there is an inverse relationship between tIgE level and age.展开更多
文摘As a matter of fact, infectious diseases hamper everyone’s life and produce a lifelong threat to everyone neutral of age, sex, lifestyle and socio-economic status. Nowadays, come into the sight of Chikungunya viral (CHIKV) infection injured many Asian and African countries, also deliberated threat in rising countries and also low socio-economic countries. CHIKV is a positive-sense, enveloped single-stranded, RNA virus belonging to the genus Alphavirus, family Togaviridae. As the Dengue & Chikungunya viruses are spread simultaneously at the same time, so it is tough to identify them. In our resource-limited countries, swift detection of CHIKV by RT-LAMP is the simplest molecular technique in low-equipment settings without the use of any expensive decoration. Heat-treated centrifuged and uncentrifuged samples were used in this study and they showed the same result (100%). Different instruments like heat block, water bath, conventional thermal cycler & real-time thermal cycler were used to amplify the CHIKV RNA and they indicated that 100% samples were identified by all four instruments. The amplified products were visualized by turbidity test, color change by HNB, step-ladder band pattern in agarose gel electrophoresis and amplification curve in real-time thermal cycler naked eye, here the results also showed 100% samples were determined by all visualized methods. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a recent technique for amplifying RNA under limited temperature, with high tangibility, quickness and competency. To identify the CHIKV RNA Reverse transcription loop-mediated isothermal amplification was fabricated and validated, and the results were also compared with reverse transcription polymerase chain reaction (RT-PCR). The sensitivity was 95.71% and specificity was 100%, these results indicate that RT-LAMP is a feasible method for quick detection of CHIKV RNA.
文摘Allergy is an adverse immunologic response of increasing prevalence and severity in which immunoglobulin E plays a pivotal role. Total IgE (tIgE) levels show wide variations depending on various factors, such as age, sex, ethnicity, and geographic area. This study set out to quantitatively determine the serum tIgE levels in Bangladeshi population who were clinically suspected cases of allergic diseases and to find out the association of tIgE level with their age and gender. This cross-sectional descriptive exploratory study was conducted at Biomed Diagnostics Laboratory, Panthapath, Dhaka, from March 2021-September 2021. A total of 402 blood samples were collected from clinically suspected cases of allergic diseases. Quantitative detection of tIgE in serum was done by Immunoblot assay manufactured by Proteome Tech Inc. (PROTIATM Allergy-Q64S panel). Among 402 subjects, a wide variation of tIgE levels ranging from 5 to 2000 IU/ml was found but majority of the study population (30.35%) showed tIgE levels within the range of 201 - 350 IU/ml. The study population aging 1 - 10 years showed highest frequency (23.63%) in tIgE concentrations and the mean tIgE level exhibited a declining tendency with an increase in age. Depending on gender there was no difference in tIgE levels. Determination of tIgE level is imperative for screening allergic diseases. The present study enlightened the distribution of tIgE levels and suggested that among Bangladeshi subjects irrespective of gender there is an inverse relationship between tIgE level and age.
