The β-1,3-glucan recognition protein gene from Spodoptera exigua （SeβGRP） was cloned and characterized. The cDNA of this gene is 1？644 nucleotides in length and the predicted polypeptide is 491 amino acids （aa）...The β-1,3-glucan recognition protein gene from Spodoptera exigua （SeβGRP） was cloned and characterized. The cDNA of this gene is 1？644 nucleotides in length and the predicted polypeptide is 491 amino acids （aa） in length, with a calculated molecular mass of 54.8 kDa. The first 22 aa encode a predicted secretion signal peptide. A BLAST search, multiple sequence alignment, and phylogenetic analysis of the aa sequence of SeβGRP revealed that this protein is most similar to the β-1,3-glucan recognition protein （βGRP） family of pattern recognition proteins. Using reverse-transcription polymerase chain reaction, we detected the presence of SeβGRP transcripts in the egg, larval, pupal, and adult stages of S. exigua. In addition, the SeβGRP transcript was expressed in all the tissues examined including the brain, hemocytes, fat body, intestine, and cuticle. There were no changes in SeβGRP mRNA levels in larvae infected with ultraviolet （UV）-killed Escherichia coli DH5α compared with the control larvae inoculated with the water; however, SeβGRP mRNA levels were markedly elevated 4–8 h after infection and slightly induced 12–24 h after infection in larvae injected with UV-killed Fusarium oxysporum. This may be because β-1,3-glucan is the main component of the cell wall of F. oxysporum, but not E. coli DH5α.展开更多
A new putative transposon was identified in the tobacco budworm, Helio- this virescens. This transposon was characterized as a full length CORE-SINE （65 bp of ＂CORE＂ core specific nucleotide short interspersed elem...A new putative transposon was identified in the tobacco budworm, Helio- this virescens. This transposon was characterized as a full length CORE-SINE （65 bp of ＂CORE＂ core specific nucleotide short interspersed elements） that resembled sequences from three other lepidopterans and humans. In particular, the A-box and B-box regions of this sequence most closely conformed to the signature of CORE-SINEs from widely divergent species. This CORE-SINE was present as a polymorphism in a hypervariable region of the gene hscp, which is the target of pyrethroid insecticides and other xenobiotics in the nerve axon. We described this new putative transposon as Noct-1 due to its presence in a noctuid moth. This is the first description of a full-length CORE-SINE with the A-box, B-box, target site duplication, and candidate core domain from an insect.展开更多
文摘The β-1,3-glucan recognition protein gene from Spodoptera exigua （SeβGRP） was cloned and characterized. The cDNA of this gene is 1？644 nucleotides in length and the predicted polypeptide is 491 amino acids （aa） in length, with a calculated molecular mass of 54.8 kDa. The first 22 aa encode a predicted secretion signal peptide. A BLAST search, multiple sequence alignment, and phylogenetic analysis of the aa sequence of SeβGRP revealed that this protein is most similar to the β-1,3-glucan recognition protein （βGRP） family of pattern recognition proteins. Using reverse-transcription polymerase chain reaction, we detected the presence of SeβGRP transcripts in the egg, larval, pupal, and adult stages of S. exigua. In addition, the SeβGRP transcript was expressed in all the tissues examined including the brain, hemocytes, fat body, intestine, and cuticle. There were no changes in SeβGRP mRNA levels in larvae infected with ultraviolet （UV）-killed Escherichia coli DH5α compared with the control larvae inoculated with the water; however, SeβGRP mRNA levels were markedly elevated 4–8 h after infection and slightly induced 12–24 h after infection in larvae injected with UV-killed Fusarium oxysporum. This may be because β-1,3-glucan is the main component of the cell wall of F. oxysporum, but not E. coli DH5α.
文摘A new putative transposon was identified in the tobacco budworm, Helio- this virescens. This transposon was characterized as a full length CORE-SINE （65 bp of ＂CORE＂ core specific nucleotide short interspersed elements） that resembled sequences from three other lepidopterans and humans. In particular, the A-box and B-box regions of this sequence most closely conformed to the signature of CORE-SINEs from widely divergent species. This CORE-SINE was present as a polymorphism in a hypervariable region of the gene hscp, which is the target of pyrethroid insecticides and other xenobiotics in the nerve axon. We described this new putative transposon as Noct-1 due to its presence in a noctuid moth. This is the first description of a full-length CORE-SINE with the A-box, B-box, target site duplication, and candidate core domain from an insect.
