Objective: To investigate the comparative effects of Diospyros blancoi(Ebenaceae)leaves(DBL), root bark(DBRB) and stem bark(DBSB) on free radicals and cancer.Methods: The polyphenol contents, antioxidant and free radi...Objective: To investigate the comparative effects of Diospyros blancoi(Ebenaceae)leaves(DBL), root bark(DBRB) and stem bark(DBSB) on free radicals and cancer.Methods: The polyphenol contents, antioxidant and free radical scavenging properties were determined using standard spectrophotometric methods. Cytotoxicity and anticancer activities were performed on brine shrimp nauplii and Ehrlich ascite carcinoma cells, respectively.Results: Among the extracts, DBSB showed the highest total antioxidant capacity and reducing capacity on ferrous ion. Based on 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radical scavenging activities, DBSB showed(95.760 ± 0.343)% and(67.460 ± 2.641)%scavenging with IC50of(3.10 ± 0.17) and(50.00 ± 3.11) mg/m L, respectively. The IC50 values of standard butylated hydroxytoluene and catechin(CA) for 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals were(8.50 ± 0.25) and(75.00 ± 0.14) mg/m L,respectively suggesting that DBSB had a significant(P < 0.05) radical scavenging activity than standards. In lipid peroxidation inhibition assay, the inhibitory activity of the extracts and the standard was in the following order: DBSB > DBRB > CA > DBL.Also, the phenolic [(139.91 ± 3.924) mg gallic acid equivalent/g] and flavonoid contents[(412.00 ± 16.70) mg catechin equivalent/g)] of DBSB were higher than that of other extracts. In addition, the DBSB showed the moderate cytotoxic and anticancer properties.Conclusions: Our results indicate that Diospyros blancoi stem bark had the significant highest antioxidant and free radical scavenging properties as well as moderate anticancer activity.Hence, we assume that the anticancer activity of this plant can be, at least in part, attributed to its content in phenolic compounds as well as its significant free radical scavenging properties.展开更多
基金Supported by the Ministry of Science and Technology(Memorandum No.39.012.002.02.01.016.2013-328)Bangladesh and University Grant Commission(Memorandum No.UGC/BUDGET/2013/7353)
文摘Objective: To investigate the comparative effects of Diospyros blancoi(Ebenaceae)leaves(DBL), root bark(DBRB) and stem bark(DBSB) on free radicals and cancer.Methods: The polyphenol contents, antioxidant and free radical scavenging properties were determined using standard spectrophotometric methods. Cytotoxicity and anticancer activities were performed on brine shrimp nauplii and Ehrlich ascite carcinoma cells, respectively.Results: Among the extracts, DBSB showed the highest total antioxidant capacity and reducing capacity on ferrous ion. Based on 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radical scavenging activities, DBSB showed(95.760 ± 0.343)% and(67.460 ± 2.641)%scavenging with IC50of(3.10 ± 0.17) and(50.00 ± 3.11) mg/m L, respectively. The IC50 values of standard butylated hydroxytoluene and catechin(CA) for 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals were(8.50 ± 0.25) and(75.00 ± 0.14) mg/m L,respectively suggesting that DBSB had a significant(P < 0.05) radical scavenging activity than standards. In lipid peroxidation inhibition assay, the inhibitory activity of the extracts and the standard was in the following order: DBSB > DBRB > CA > DBL.Also, the phenolic [(139.91 ± 3.924) mg gallic acid equivalent/g] and flavonoid contents[(412.00 ± 16.70) mg catechin equivalent/g)] of DBSB were higher than that of other extracts. In addition, the DBSB showed the moderate cytotoxic and anticancer properties.Conclusions: Our results indicate that Diospyros blancoi stem bark had the significant highest antioxidant and free radical scavenging properties as well as moderate anticancer activity.Hence, we assume that the anticancer activity of this plant can be, at least in part, attributed to its content in phenolic compounds as well as its significant free radical scavenging properties.