Primary hepatocytes(PHCs)are widely used in various fields,but the progressive deterioration of liverspecific features in vitro significantly limits their application.While the transcriptional regulation and whole cel...Primary hepatocytes(PHCs)are widely used in various fields,but the progressive deterioration of liverspecific features in vitro significantly limits their application.While the transcriptional regulation and whole cell proteome(WCP)of PHCs have been extensively studied,only a small number of studies have addressed the role of posttranslational modifications in this process.To elucidate the underlying mechanisms that induce dedifferentiation,we carried out parallel quantifications of the transcriptome,WCP,ubiquitinome,and phosphoproteome of rat PHCs after 0,6,12,24,and 48 h of in vitro culture.Our data constitute a detailed proteomic analysis of dedifferentiated PHCs including 2196 proteins,2056 ubiquitinated sites,and 4932 phosphorylated peptides.We revealed a low correlation between the transcriptome and WCP during dedifferentiation.A combined analysis of the ubiquitinome with the corresponding WCP indicated that the dedifferentiation of PHCs led to an increase in nondegradative K27 ubiquitination.Functional analysis of the altered phosphoproteins suggested a significant enrichment in ferroptosis.In all,404 proteins with both ubiquitination and phosphorylation were identified to be involved in critical metabolic events.Furthermore,Ptbph Hnqjd,Hnrnpu,and Srrm2 were identified as hub genes.Taken together,our data provide new insights into proteome dynamics during PHC dedifferentiation and potential targets to inhibit the dedifferentiation process.展开更多
Since there are multiple influencing factors and lack of evaluation standards for the construction of hydrogen production and hydrogenation station in China,this paper establishes the suitability evaluation index syst...Since there are multiple influencing factors and lack of evaluation standards for the construction of hydrogen production and hydrogenation station in China,this paper establishes the suitability evaluation index system of hydrogen production and hydrogenation station from four aspects of technology,economy,environment and safety.Combined with actual conditions,this paper uses Analytic Hierarch Process(AHP)and Fuzzy Comprehensive eval-uation to evaluate water electrolysis hydrogen and hydrogenation production station and natural gas reformation hydrogen production and hydrogenation station.The results show that hydrogen producing by water electrolysis is more efficient than natural gas reformation in hydrogen production and hydrogenation station,and it should be choose firstly.Furthermore,natural gas reformation hydrogen producing is inferior in technology,environment and safety,but it is superior to water electrolysis hydrogen producing in economy.In the future,we can strengthen development of the hydrogen production from renewable energy sources,so as to enhance the economic benefit of that by water electrolysis.In addition,we can also adopt two ways of hydrogen production in hydrogen production and hydrogenation station to achieve favorable integrative benefits.展开更多
A reversed phase (RP)/hydrophilic interaction (HILIC)/ion exchange (IEX) mixed tri-mode stationary phase (TMSP) has been prepared via a divergent synthesis scheme starting from propylamine on silica then by amine-epox...A reversed phase (RP)/hydrophilic interaction (HILIC)/ion exchange (IEX) mixed tri-mode stationary phase (TMSP) has been prepared via a divergent synthesis scheme starting from propylamine on silica then by amine-epoxy reactions with 1,4-butanedioldiglycidyl ether and tertiary amines (N,Ndimethyldecylamine, DMDA). Its retention mechanism was found to follow RP/HILIC/IEX mixed-mode.The stop-flow test revealed that TMSP had good compatibility with 100% aqueous mobile phase. It demonstrated effective separation towards several kinds of compounds or drug molecules and their counterions within a single run.展开更多
Posttranslational modifications(PTMs)of proteins,particularly acetylation,phosphorylation,and ubiquitination,play critical roles in the host innate immune response.PTMs’dynamic changes and the crosstalk among them ar...Posttranslational modifications(PTMs)of proteins,particularly acetylation,phosphorylation,and ubiquitination,play critical roles in the host innate immune response.PTMs’dynamic changes and the crosstalk among them are complicated.To build a comprehensive dynamic network of inflammation-related proteins,we integrated data from the whole-cell proteome(WCP),acetylome,phosphoproteome,and ubiquitinome of human and mouse macrophages.Our datasets of acetylation,phosphorylation,and ubiquitination sites helped identify PTM crosstalk within and across proteins involved in the inflammatory response.Stimulation of macrophages by lipopolysaccharide(LPS)resulted in both degradative and non-degradative ubiquitination.Moreover,this study contributes to the interpretation of the roles of known inflammatory molecules and the discovery of novel inflammatory proteins.展开更多
The assembly of biomacromole particles with artificial solid matrixes to fabricate hybrid composites has been demonstrated as an efficient strategy to promote both components'applications.Elaborating matrixes as w...The assembly of biomacromole particles with artificial solid matrixes to fabricate hybrid composites has been demonstrated as an efficient strategy to promote both components'applications.Elaborating matrixes as well as appropriate assembly method is essential for optimal performances of the obtained natural-artificial composites.Herein,we fabricated the Cyt c@COF-42-B microcapsule through a sacrificial templating method and researched their adjustable biocatalysis by precisely adjusting the microenvironments of these assembled enzyme@COF particles.As a result,the hydrophobicity and mass transfer of the COF microcapsule can be controlled by modifying the different amounts of PEG moieties,thus improving the composite's bioactivity.We found that compared with pristine Cyt c@COF-42-B without PEG,the Cyt c@COF-42-B-PEG exhibited a~2.2 times higher activity.This study provides a significant step toward designing customizable functional natural-artificial composites for enhanced biomacromolecule's activity.展开更多
A new species, Hemithyrsocera hemiptera sp. nov., is described from Southwest China, based on its shortened tegmina, and the lack of hind wings, including the illustrations of its habitus and male genitalia. A key to ...A new species, Hemithyrsocera hemiptera sp. nov., is described from Southwest China, based on its shortened tegmina, and the lack of hind wings, including the illustrations of its habitus and male genitalia. A key to the Chinese species of the genus is provided. The type material is deposited in Shanghai Entomological Museum(SEM), Chinese Academy of Sciences(CAS).展开更多
基金This work was supported by the National Key Research and Development Program of China(2016YFC1101304/3)the National Natural Science Foundation of China(81400589,81790630,and 81790633)Research Unit of Infectious Diseases and Microecology,Chinese Academy of Medical Sciences(2019RU021).
文摘Primary hepatocytes(PHCs)are widely used in various fields,but the progressive deterioration of liverspecific features in vitro significantly limits their application.While the transcriptional regulation and whole cell proteome(WCP)of PHCs have been extensively studied,only a small number of studies have addressed the role of posttranslational modifications in this process.To elucidate the underlying mechanisms that induce dedifferentiation,we carried out parallel quantifications of the transcriptome,WCP,ubiquitinome,and phosphoproteome of rat PHCs after 0,6,12,24,and 48 h of in vitro culture.Our data constitute a detailed proteomic analysis of dedifferentiated PHCs including 2196 proteins,2056 ubiquitinated sites,and 4932 phosphorylated peptides.We revealed a low correlation between the transcriptome and WCP during dedifferentiation.A combined analysis of the ubiquitinome with the corresponding WCP indicated that the dedifferentiation of PHCs led to an increase in nondegradative K27 ubiquitination.Functional analysis of the altered phosphoproteins suggested a significant enrichment in ferroptosis.In all,404 proteins with both ubiquitination and phosphorylation were identified to be involved in critical metabolic events.Furthermore,Ptbph Hnqjd,Hnrnpu,and Srrm2 were identified as hub genes.Taken together,our data provide new insights into proteome dynamics during PHC dedifferentiation and potential targets to inhibit the dedifferentiation process.
基金supported by the Major National Key R&D Program of China 2018YFF0214804Chinese Academy of Engineering Consulting Project“Research on China’s Hydrogen Energy and Fuel Cell Development Strategy”(2019-ZD-3).
文摘Since there are multiple influencing factors and lack of evaluation standards for the construction of hydrogen production and hydrogenation station in China,this paper establishes the suitability evaluation index system of hydrogen production and hydrogenation station from four aspects of technology,economy,environment and safety.Combined with actual conditions,this paper uses Analytic Hierarch Process(AHP)and Fuzzy Comprehensive eval-uation to evaluate water electrolysis hydrogen and hydrogenation production station and natural gas reformation hydrogen production and hydrogenation station.The results show that hydrogen producing by water electrolysis is more efficient than natural gas reformation in hydrogen production and hydrogenation station,and it should be choose firstly.Furthermore,natural gas reformation hydrogen producing is inferior in technology,environment and safety,but it is superior to water electrolysis hydrogen producing in economy.In the future,we can strengthen development of the hydrogen production from renewable energy sources,so as to enhance the economic benefit of that by water electrolysis.In addition,we can also adopt two ways of hydrogen production in hydrogen production and hydrogenation station to achieve favorable integrative benefits.
基金financially supported by the National Natural Science Foundation of China(Nos. 21477037, 21322502)the Outstanding Young Talent Cultivation Fund of East China University of Science and Technology
文摘A reversed phase (RP)/hydrophilic interaction (HILIC)/ion exchange (IEX) mixed tri-mode stationary phase (TMSP) has been prepared via a divergent synthesis scheme starting from propylamine on silica then by amine-epoxy reactions with 1,4-butanedioldiglycidyl ether and tertiary amines (N,Ndimethyldecylamine, DMDA). Its retention mechanism was found to follow RP/HILIC/IEX mixed-mode.The stop-flow test revealed that TMSP had good compatibility with 100% aqueous mobile phase. It demonstrated effective separation towards several kinds of compounds or drug molecules and their counterions within a single run.
基金supported by the National Key R&D Program of China(Grant Nos.2016YFC1101304/3 and 2017YFC1200100)the National Natural Science Foundation of China(Grant No.81400589)+1 种基金the National S&T Major Project,China(Grant No.2017ZX10202202-001-008)the Science Fund for Creative Research Groups of the National Natural Science Foundation,China(Grant No.81721091)。
文摘Posttranslational modifications(PTMs)of proteins,particularly acetylation,phosphorylation,and ubiquitination,play critical roles in the host innate immune response.PTMs’dynamic changes and the crosstalk among them are complicated.To build a comprehensive dynamic network of inflammation-related proteins,we integrated data from the whole-cell proteome(WCP),acetylome,phosphoproteome,and ubiquitinome of human and mouse macrophages.Our datasets of acetylation,phosphorylation,and ubiquitination sites helped identify PTM crosstalk within and across proteins involved in the inflammatory response.Stimulation of macrophages by lipopolysaccharide(LPS)resulted in both degradative and non-degradative ubiquitination.Moreover,this study contributes to the interpretation of the roles of known inflammatory molecules and the discovery of novel inflammatory proteins.
基金financial support from the National Natural Science Foundation of China(grant Nos.21871153,31800793)Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(TSBICIP-KJGG-003).
文摘The assembly of biomacromole particles with artificial solid matrixes to fabricate hybrid composites has been demonstrated as an efficient strategy to promote both components'applications.Elaborating matrixes as well as appropriate assembly method is essential for optimal performances of the obtained natural-artificial composites.Herein,we fabricated the Cyt c@COF-42-B microcapsule through a sacrificial templating method and researched their adjustable biocatalysis by precisely adjusting the microenvironments of these assembled enzyme@COF particles.As a result,the hydrophobicity and mass transfer of the COF microcapsule can be controlled by modifying the different amounts of PEG moieties,thus improving the composite's bioactivity.We found that compared with pristine Cyt c@COF-42-B without PEG,the Cyt c@COF-42-B-PEG exhibited a~2.2 times higher activity.This study provides a significant step toward designing customizable functional natural-artificial composites for enhanced biomacromolecule's activity.
基金sponsored by the Science-technology basic condition platform from The Ministry of Science and Technology of People’s Republic of China(2005DKA21402)
文摘A new species, Hemithyrsocera hemiptera sp. nov., is described from Southwest China, based on its shortened tegmina, and the lack of hind wings, including the illustrations of its habitus and male genitalia. A key to the Chinese species of the genus is provided. The type material is deposited in Shanghai Entomological Museum(SEM), Chinese Academy of Sciences(CAS).
