Leachate plumes from landfills are a major source of pollution in Nigeria, especially in urban areas. Assessing leachate contamination in the subsoil is considered a complex process that needs detailed field measureme...Leachate plumes from landfills are a major source of pollution in Nigeria, especially in urban areas. Assessing leachate contamination in the subsoil is considered a complex process that needs detailed field measurement to accurately define the extent of contamination. To ascertain the extent of pollution of the subsoil and groundwater sources which were reportedly contaminated by leachate plumes from an old dumpsite located in Osubi town, an integrated geo-electrical method involving 1-D vertical electrical sounding (VES) and 2-D, 3-D ERT techniques were employed. Orthogonal set of 2-D apparent resistivity data was collected in a 100 × 50 m<sup>2</sup> rectangular grid around the dumpsite, using the Wenner array. Two years later, three (3) 2-D resistivity imaging profiles were also recorded in time-lapse mode at the dumpsite to monitor the possible effects of attenuation on the leachate over time. Ten (10) VES data were also acquired and used along with the 2-D imaging data. 2-D apparent resistivity data were inverted with Dipprowin software program. The orthogonal set of 2-D lines of apparent resistivity was merged into 3-D data and inverted with RES3DINV program to create a 3-D subsurface resistivity model. Geological models observed from 2-D and 3-D resistivity inversion revealed low resistivity values in the order ρ Ω·m which is indicative of leachate plumes in the saturation zone (pore water). The 2-D resistivity-depth sections imaged low resistivity leachate plumes at the near surface (<5 m) to a depth of 25.0 m, while 3-D inversion depth slices imaged leachate contaminant within the first, second and third layers at depth ranging from 0.00 - 2.50 m, 2.50 - 5.38 m and 5.38 - 8.68 m respectively. Thus, leachate contamination clearly increased with depth beyond the depth of first and second aquifers in the area which implies that available groundwater for domestic use is already contaminated with leachate from the dumpsite. Leachate contaminant-depth map estimated for the second geoelectric layers for VES 2, 3, 4, 7, 8, 9, and 10 shows that the second layer has been invaded completely by leachate contamination up to 6.5 m depth. 2-D apparent resistivity data acquired two years after show lower resistivity anomalies of the leachate plume caused by time-lapse attenuation effect on the observed resistivity of the leachate. This indicates that the leachate plume has become more conductive and toxic to the environment. The Longitudinal conductance map of the area shows that the aquifer protective capacity of this area is weak (0.1 - 0.19 Mho) thus, aquifers in the area are prone to pollution from the dumpsite. The three techniques used in this study (2-D, 3-D ERT and 1-D VES) fitly provided crucial information on the degree of contamination caused by the landfill leachate plume. Therefore, it is advisable to implement an environmental remediation and leachate management program.展开更多
Purpose: There is a need for a continued effort to develop rapid immunodiagnostic assays for tuberculosis (TB) infection with greater sensitivity and specificity that can be used in the field and in the laboratory and...Purpose: There is a need for a continued effort to develop rapid immunodiagnostic assays for tuberculosis (TB) infection with greater sensitivity and specificity that can be used in the field and in the laboratory and that can be formatted for use with multiple species. This would help to obtain definitive early diagnosis of TB. The present study was developed to determine the role of using early secreted antigenic target-6 (ESAT-6) in immunodiagnosis of Mycobacterium tuberculosis. Methods: Serum samples were obtained from TB infected patients and normal healthy controls. Two rapid immunodiagnostic assays (Enzyme-linked immunosorbent assay (ELISA) and Immunoblotting) were performed. Results: The sensitivity of immunoblotting assay was 100%;however, ESAT-6 antigen was not able to discriminate between patients and normal controls. Application of direct ELISA using ESAT-6 antigen yielded 97.6% sensitivity and 75% specificity for the diagnosis of TB infection. Conclusion: In conclusion, the detection of antibodies against ESAT-6 antigen in the sera of TB patients by direct ELISA could be used as a preliminary assay for diagnosis of human M. tuberculosis infection. A combination of the ELISA with either radiological or microscopic examination is required to overcome the low specificity of the assay for negative results.展开更多
文摘Leachate plumes from landfills are a major source of pollution in Nigeria, especially in urban areas. Assessing leachate contamination in the subsoil is considered a complex process that needs detailed field measurement to accurately define the extent of contamination. To ascertain the extent of pollution of the subsoil and groundwater sources which were reportedly contaminated by leachate plumes from an old dumpsite located in Osubi town, an integrated geo-electrical method involving 1-D vertical electrical sounding (VES) and 2-D, 3-D ERT techniques were employed. Orthogonal set of 2-D apparent resistivity data was collected in a 100 × 50 m<sup>2</sup> rectangular grid around the dumpsite, using the Wenner array. Two years later, three (3) 2-D resistivity imaging profiles were also recorded in time-lapse mode at the dumpsite to monitor the possible effects of attenuation on the leachate over time. Ten (10) VES data were also acquired and used along with the 2-D imaging data. 2-D apparent resistivity data were inverted with Dipprowin software program. The orthogonal set of 2-D lines of apparent resistivity was merged into 3-D data and inverted with RES3DINV program to create a 3-D subsurface resistivity model. Geological models observed from 2-D and 3-D resistivity inversion revealed low resistivity values in the order ρ Ω·m which is indicative of leachate plumes in the saturation zone (pore water). The 2-D resistivity-depth sections imaged low resistivity leachate plumes at the near surface (<5 m) to a depth of 25.0 m, while 3-D inversion depth slices imaged leachate contaminant within the first, second and third layers at depth ranging from 0.00 - 2.50 m, 2.50 - 5.38 m and 5.38 - 8.68 m respectively. Thus, leachate contamination clearly increased with depth beyond the depth of first and second aquifers in the area which implies that available groundwater for domestic use is already contaminated with leachate from the dumpsite. Leachate contaminant-depth map estimated for the second geoelectric layers for VES 2, 3, 4, 7, 8, 9, and 10 shows that the second layer has been invaded completely by leachate contamination up to 6.5 m depth. 2-D apparent resistivity data acquired two years after show lower resistivity anomalies of the leachate plume caused by time-lapse attenuation effect on the observed resistivity of the leachate. This indicates that the leachate plume has become more conductive and toxic to the environment. The Longitudinal conductance map of the area shows that the aquifer protective capacity of this area is weak (0.1 - 0.19 Mho) thus, aquifers in the area are prone to pollution from the dumpsite. The three techniques used in this study (2-D, 3-D ERT and 1-D VES) fitly provided crucial information on the degree of contamination caused by the landfill leachate plume. Therefore, it is advisable to implement an environmental remediation and leachate management program.
文摘Purpose: There is a need for a continued effort to develop rapid immunodiagnostic assays for tuberculosis (TB) infection with greater sensitivity and specificity that can be used in the field and in the laboratory and that can be formatted for use with multiple species. This would help to obtain definitive early diagnosis of TB. The present study was developed to determine the role of using early secreted antigenic target-6 (ESAT-6) in immunodiagnosis of Mycobacterium tuberculosis. Methods: Serum samples were obtained from TB infected patients and normal healthy controls. Two rapid immunodiagnostic assays (Enzyme-linked immunosorbent assay (ELISA) and Immunoblotting) were performed. Results: The sensitivity of immunoblotting assay was 100%;however, ESAT-6 antigen was not able to discriminate between patients and normal controls. Application of direct ELISA using ESAT-6 antigen yielded 97.6% sensitivity and 75% specificity for the diagnosis of TB infection. Conclusion: In conclusion, the detection of antibodies against ESAT-6 antigen in the sera of TB patients by direct ELISA could be used as a preliminary assay for diagnosis of human M. tuberculosis infection. A combination of the ELISA with either radiological or microscopic examination is required to overcome the low specificity of the assay for negative results.