After China and Australia established diplomatic ties in 1972, bilateral relations developed rapidly. People-to-people links, politicalexchanges and trade and economic cooperation played an increasingly larger part in...After China and Australia established diplomatic ties in 1972, bilateral relations developed rapidly. People-to-people links, politicalexchanges and trade and economic cooperation played an increasingly larger part in the foreign policies of the two countries. In the post global financial crisis era, bilateral relations have remained positive and played an imPortant role in ensuring the stability and prosperity of the Pacific region. There have been several unavoidable misunderstandings and even disagreements between the two countries, which has influenced mutual trust and policies. This article will aim to analyze the history and current situation of the Sino-Australian relationship in an objective and comprehensive way; to look ahead to future bilateral relations, and make policy recommendations for the further development of bilateral ties to increase mutual understanding and promote a more positive future for Sino-Australian relations.展开更多
目的:建立基于重组酶聚合酶扩增技术(RPA)技术快速检测肺炎支原体的方法。方法:本研究以肺炎支原体编码P1黏附蛋白为靶基因,利用Primer Premier 5软件进行引物、探针的设计,最终筛选出最佳引物。同时设计相应的实时荧光定量PCR(RT-PCR)...目的:建立基于重组酶聚合酶扩增技术(RPA)技术快速检测肺炎支原体的方法。方法:本研究以肺炎支原体编码P1黏附蛋白为靶基因,利用Primer Premier 5软件进行引物、探针的设计,最终筛选出最佳引物。同时设计相应的实时荧光定量PCR(RT-PCR)引物用于后续的验证试验。对反应体系试剂比例、反应时间、反应温度、引物探针浓度进行确定。肺炎支原体、解脲支原体、人型支原体、肺炎克雷伯菌、肺炎双球菌、大肠杆菌和链球菌作为对照评估RPA检测肺炎支原体的特异性及敏感度。结果:RPA快速检测肺炎支原体方法仅需14 min,检测灵敏度达200 copies/mL;6种非肺炎支原体均不能扩增,特异性较高。结论:本研究建立了肺炎支原体的RPA快速检测方法,具有迅速、简便、经济等优势,为肺炎支原体的快速检测提供一个新的有利工具。展开更多
Growth shapes soft tissues not only through mass addition or volume expansion but also through deformation instabilities and consequent morphological evolution.In this paper,we probe the torsion instability of an anis...Growth shapes soft tissues not only through mass addition or volume expansion but also through deformation instabilities and consequent morphological evolution.In this paper,we probe the torsion instability of an anisotropically growing tube with fiber reinforcement,which mimics many tubular organs in animals or plants.We derive the Stroh formulation for the incremental boundary value problem and numerically solve it using the surface impedance method.A linear st ability analysis is conducted to investigate the critical condition for the onset of wrinkling.The thresholds of helical wrinkling are calculated in terms of growth ratio and external load.The effect of fibers on the critical state under axial stretching is examined.It is found that the tangential growth tends to enhance the critical torsion angle but has a weak influence on the critical longitudinal mode of wrinkling,which,however,can be remarkably affected by the axial growth.Our study can help understand the formation of helical morphologies in biological mat erials and provide cues for engineering desired st ruc tu res or devices.展开更多
文摘After China and Australia established diplomatic ties in 1972, bilateral relations developed rapidly. People-to-people links, politicalexchanges and trade and economic cooperation played an increasingly larger part in the foreign policies of the two countries. In the post global financial crisis era, bilateral relations have remained positive and played an imPortant role in ensuring the stability and prosperity of the Pacific region. There have been several unavoidable misunderstandings and even disagreements between the two countries, which has influenced mutual trust and policies. This article will aim to analyze the history and current situation of the Sino-Australian relationship in an objective and comprehensive way; to look ahead to future bilateral relations, and make policy recommendations for the further development of bilateral ties to increase mutual understanding and promote a more positive future for Sino-Australian relations.
文摘目的:建立基于重组酶聚合酶扩增技术(RPA)技术快速检测肺炎支原体的方法。方法:本研究以肺炎支原体编码P1黏附蛋白为靶基因,利用Primer Premier 5软件进行引物、探针的设计,最终筛选出最佳引物。同时设计相应的实时荧光定量PCR(RT-PCR)引物用于后续的验证试验。对反应体系试剂比例、反应时间、反应温度、引物探针浓度进行确定。肺炎支原体、解脲支原体、人型支原体、肺炎克雷伯菌、肺炎双球菌、大肠杆菌和链球菌作为对照评估RPA检测肺炎支原体的特异性及敏感度。结果:RPA快速检测肺炎支原体方法仅需14 min,检测灵敏度达200 copies/mL;6种非肺炎支原体均不能扩增,特异性较高。结论:本研究建立了肺炎支原体的RPA快速检测方法,具有迅速、简便、经济等优势,为肺炎支原体的快速检测提供一个新的有利工具。
基金Supports from National Natural Science Foundation of China(Grant Nos.11432008,11672161,and 11620101001)are acknowledged.
文摘Growth shapes soft tissues not only through mass addition or volume expansion but also through deformation instabilities and consequent morphological evolution.In this paper,we probe the torsion instability of an anisotropically growing tube with fiber reinforcement,which mimics many tubular organs in animals or plants.We derive the Stroh formulation for the incremental boundary value problem and numerically solve it using the surface impedance method.A linear st ability analysis is conducted to investigate the critical condition for the onset of wrinkling.The thresholds of helical wrinkling are calculated in terms of growth ratio and external load.The effect of fibers on the critical state under axial stretching is examined.It is found that the tangential growth tends to enhance the critical torsion angle but has a weak influence on the critical longitudinal mode of wrinkling,which,however,can be remarkably affected by the axial growth.Our study can help understand the formation of helical morphologies in biological mat erials and provide cues for engineering desired st ruc tu res or devices.
