DEAR EDITOR,The Antarctic toothfish,Dissostichus mawsoni,belongs to the Nototheniidae family and is distributed in sub-zero temperatures below S60°latitude in the Southern Ocean.Therefore,it is an attractive mode...DEAR EDITOR,The Antarctic toothfish,Dissostichus mawsoni,belongs to the Nototheniidae family and is distributed in sub-zero temperatures below S60°latitude in the Southern Ocean.Therefore,it is an attractive model species to study the stenothermal cold-adapted character state.In this study,we successfully generated highly contiguous genome sequences of D.mawsoni,which contained 1062 scaffolds with a N50 length of 36.98 Mb and longest scaffold length of 46.82 Mb.Repetitive elements accounted for 40.87%of the genome.We also inferred 32914 protein-coding genes using in silico gene prediction and transcriptome sequencing and detected splicing variants using Isoform-Sequencing(Iso-Seq),which will be invaluable resource for further exploration of the adaptation mechanisms of Antarctic toothfish.This new high-quality reference genome of D.mawsoni provides a fundamental resource for a deeper understanding of cold adaptation and conservation of species.展开更多
基金This study was supported by a grant from the National Institute of Fisheries Science(NIFS)of the Republic of Korea(R2019021)and“Ecosystem Structure and Function of Marine Protected Area(MPA)in Antarctica”project(PM19060)funded by the Ministry of Oceans and Fisheries(20170336),Korea。
文摘DEAR EDITOR,The Antarctic toothfish,Dissostichus mawsoni,belongs to the Nototheniidae family and is distributed in sub-zero temperatures below S60°latitude in the Southern Ocean.Therefore,it is an attractive model species to study the stenothermal cold-adapted character state.In this study,we successfully generated highly contiguous genome sequences of D.mawsoni,which contained 1062 scaffolds with a N50 length of 36.98 Mb and longest scaffold length of 46.82 Mb.Repetitive elements accounted for 40.87%of the genome.We also inferred 32914 protein-coding genes using in silico gene prediction and transcriptome sequencing and detected splicing variants using Isoform-Sequencing(Iso-Seq),which will be invaluable resource for further exploration of the adaptation mechanisms of Antarctic toothfish.This new high-quality reference genome of D.mawsoni provides a fundamental resource for a deeper understanding of cold adaptation and conservation of species.
