Saliva is a feasible specimen used in sample collection for research purposes.It is reported that saliva typically contains^430,000 epithelial cells per mL.There are various cost-effective methods and commercial kits ...Saliva is a feasible specimen used in sample collection for research purposes.It is reported that saliva typically contains^430,000 epithelial cells per mL.There are various cost-effective methods and commercial kits available which are used to extract DNA from saliva.However,this study aims to explore a simple,time-saving and cheap method of DNA extraction using daily life household chemicals without the help of any electronic equipment.This protocol utilizes pineapple juice which contains bromelain enzyme as a proteolytic agent and dishwashing liquid as a detergent to rupture the cell membrane and remove the contamination.It takes approximately 10-15 min for the completion of extraction procedure.Results have indicated the good quality of DNA by gel electrophoresis and good yield with purity of 1.7-1.8 optical density.Thus,this method serves as a simple,convenient,easy-to-handle,time-saving,cheap and less laborious way for the extraction of DNA in daily routine practices with a good precision of quality and quantity that could be used for diagnostic purposes.展开更多
文摘Saliva is a feasible specimen used in sample collection for research purposes.It is reported that saliva typically contains^430,000 epithelial cells per mL.There are various cost-effective methods and commercial kits available which are used to extract DNA from saliva.However,this study aims to explore a simple,time-saving and cheap method of DNA extraction using daily life household chemicals without the help of any electronic equipment.This protocol utilizes pineapple juice which contains bromelain enzyme as a proteolytic agent and dishwashing liquid as a detergent to rupture the cell membrane and remove the contamination.It takes approximately 10-15 min for the completion of extraction procedure.Results have indicated the good quality of DNA by gel electrophoresis and good yield with purity of 1.7-1.8 optical density.Thus,this method serves as a simple,convenient,easy-to-handle,time-saving,cheap and less laborious way for the extraction of DNA in daily routine practices with a good precision of quality and quantity that could be used for diagnostic purposes.
