Objective:The alcoholic extract of stem bark of Terminalia arjuna（ALTA） was screened for antioxidant and antimutagenic（anticlastogenic） activity.Methods:Antioxidant property was determined by l,l,Diphynyl,2-Picryl...Objective:The alcoholic extract of stem bark of Terminalia arjuna（ALTA） was screened for antioxidant and antimutagenic（anticlastogenic） activity.Methods:Antioxidant property was determined by l,l,Diphynyl,2-Picryl hydrazyl（DPPH） assay,super oxide radical scavenging activity,lipid peroxidation assay and total polyphenolic content was determined by Folin-Ciocalteau’s reagent.Antimutagenic activity was evaluated using micronucleus test in mice. Results:The ALTA has shown potent antioxidant activity with EC<sub>50</sub> of 2.491±0.160,50.110±0.150 & 71.000±0.250 in DPPH assay,superoxide radical scavenging activity and lipid peroxidation assay, which is comparable with ascorbic acid with EC<sub>50</sub> of 2.471±0.140,40.500±0.390 and 63.000±0.360 respectively.In micronucleus test,ALTA（100 & 200 mg/kg,p.o.） showed significant reduction in percentage of micronucleus in both polychromatic erythrocytes（PCE） and normochromatie erythrocytes（NCE） and also shown significant reduction in P/N ratio.Conclusions:These results suggested that ALTA possess significant antioxidant and antimutagenic activity.展开更多
文摘Objective:The alcoholic extract of stem bark of Terminalia arjuna（ALTA） was screened for antioxidant and antimutagenic（anticlastogenic） activity.Methods:Antioxidant property was determined by l,l,Diphynyl,2-Picryl hydrazyl（DPPH） assay,super oxide radical scavenging activity,lipid peroxidation assay and total polyphenolic content was determined by Folin-Ciocalteau’s reagent.Antimutagenic activity was evaluated using micronucleus test in mice. Results:The ALTA has shown potent antioxidant activity with EC<sub>50</sub> of 2.491±0.160,50.110±0.150 & 71.000±0.250 in DPPH assay,superoxide radical scavenging activity and lipid peroxidation assay, which is comparable with ascorbic acid with EC<sub>50</sub> of 2.471±0.140,40.500±0.390 and 63.000±0.360 respectively.In micronucleus test,ALTA（100 & 200 mg/kg,p.o.） showed significant reduction in percentage of micronucleus in both polychromatic erythrocytes（PCE） and normochromatie erythrocytes（NCE） and also shown significant reduction in P/N ratio.Conclusions:These results suggested that ALTA possess significant antioxidant and antimutagenic activity.
