The kelp grouper (Epinephelus bruneus), belonging to one of the largest genera among the subfamily Epinephelinae, is a commercially important fish in Japan. There are limited data about the genomics of this species. T...The kelp grouper (Epinephelus bruneus), belonging to one of the largest genera among the subfamily Epinephelinae, is a commercially important fish in Japan. There are limited data about the genomics of this species. To provide tools for addressing both population genetics studies and gene mapping, dito pentanucleotide simple sequence repeat (SSR) markers were developed using 454 pyrosequencing. Among the 1466 SSR markers developed, 1244 primer sets produced strong PCR products, of which 905 (72.7%) were polymorphic in kelp grouper. Cross-species utility of the 905 polymorphic SSR markers was tested in four additional Epinephelinae species of Hyporthodus septemfasciatus, Plectropomus leopardus, Epinephelus lanceolatus and Epinephelus coioides. Results revealed that, respectively, 401 (44.3%), 136 (15.0%), 434 (49.0%) and 538 (59.4%) SSRs showed specific polymorphic products. Of these, 40 SSR markers (33 di-, 1 tri- and 6 tetra-nucleotides) showed polymorphism in all species tested. Additionally, three AGAT SSR motifs which accounted for 42.9% of the nondi-nucleotide markers were found in the 40 SSR markers. This indicates that the AGAT SSR motif has a high potential as a highly versatile SSR marker in grouper Epinephelinae. The SSR markers developed in this study can be employed to obtain reliable genetic variability estimates for groupers (Epinephelinae).展开更多
Anguillids areаvaluable fish commodity worldwide.Although Anguilla luzonensis have been abundantly found in the northern Philippines and collected for trade,no available records show that it recruited in the mid-part...Anguillids areаvaluable fish commodity worldwide.Although Anguilla luzonensis have been abundantly found in the northern Philippines and collected for trade,no available records show that it recruited in the mid-part where Lagonoy Gulf,Bicol is situated.In this study,we investigated the occurrence of A.luzonensis in the tributaries along the Lagonoy Gulf,Philippines using molecular tools.Glass eel specimens were collected in 2018-2019 from the Comun river,Albay;the Lagonoy river,Camarines Sur;and the Bato river,Catanduanes.Anguilla luzonensis was first reported in Lagonoy Gulf using molecular analysis.A.luzonensis was the second most abundant species in the Comun and Lagonoy rivers(9.5 and 22.4%,respectively).Anguilla luzonensis collected from the Comun and Lagonoy rivers did not show a significant difference(FST=0.00825,p>0.05).Anguilla marmorata was the most dominant species in all tributaries(71.1-98.0%).In the Comun and Lagonoy rivers,A.bicolor pacifica was the third most abundant species(7.7 and 6.5%,respectively).In addition,Anguilla celebesensis was only found rarely in the Comun river(0.9%).This study provides important information for sustainable resource management and effective utilization of the eel species in these regions.展开更多
文摘The kelp grouper (Epinephelus bruneus), belonging to one of the largest genera among the subfamily Epinephelinae, is a commercially important fish in Japan. There are limited data about the genomics of this species. To provide tools for addressing both population genetics studies and gene mapping, dito pentanucleotide simple sequence repeat (SSR) markers were developed using 454 pyrosequencing. Among the 1466 SSR markers developed, 1244 primer sets produced strong PCR products, of which 905 (72.7%) were polymorphic in kelp grouper. Cross-species utility of the 905 polymorphic SSR markers was tested in four additional Epinephelinae species of Hyporthodus septemfasciatus, Plectropomus leopardus, Epinephelus lanceolatus and Epinephelus coioides. Results revealed that, respectively, 401 (44.3%), 136 (15.0%), 434 (49.0%) and 538 (59.4%) SSRs showed specific polymorphic products. Of these, 40 SSR markers (33 di-, 1 tri- and 6 tetra-nucleotides) showed polymorphism in all species tested. Additionally, three AGAT SSR motifs which accounted for 42.9% of the nondi-nucleotide markers were found in the 40 SSR markers. This indicates that the AGAT SSR motif has a high potential as a highly versatile SSR marker in grouper Epinephelinae. The SSR markers developed in this study can be employed to obtain reliable genetic variability estimates for groupers (Epinephelinae).
基金the Department of Agriculture(DA)Gratuitous Permit No.0173-19.
文摘Anguillids areаvaluable fish commodity worldwide.Although Anguilla luzonensis have been abundantly found in the northern Philippines and collected for trade,no available records show that it recruited in the mid-part where Lagonoy Gulf,Bicol is situated.In this study,we investigated the occurrence of A.luzonensis in the tributaries along the Lagonoy Gulf,Philippines using molecular tools.Glass eel specimens were collected in 2018-2019 from the Comun river,Albay;the Lagonoy river,Camarines Sur;and the Bato river,Catanduanes.Anguilla luzonensis was first reported in Lagonoy Gulf using molecular analysis.A.luzonensis was the second most abundant species in the Comun and Lagonoy rivers(9.5 and 22.4%,respectively).Anguilla luzonensis collected from the Comun and Lagonoy rivers did not show a significant difference(FST=0.00825,p>0.05).Anguilla marmorata was the most dominant species in all tributaries(71.1-98.0%).In the Comun and Lagonoy rivers,A.bicolor pacifica was the third most abundant species(7.7 and 6.5%,respectively).In addition,Anguilla celebesensis was only found rarely in the Comun river(0.9%).This study provides important information for sustainable resource management and effective utilization of the eel species in these regions.
