Objective:To identify the differences in cytokine expression between sinonasal tissue from patients treated with Leptospermum (Manuka) honey (LH) irrigation versus normal saline irrigation twice-daily for twelve weeks...Objective:To identify the differences in cytokine expression between sinonasal tissue from patients treated with Leptospermum (Manuka) honey (LH) irrigation versus normal saline irrigation twice-daily for twelve weeks following sinus surgery (FESS).Methods:Forty-six CRS patients were recruited.Sinus tissue biopsies were collected during FESS and then at 5 and 12 weeks postoperatively during the course of treatment.A multiplex cytokine assay quantified the abundance of 17 cytokines in biopsied tissue.Cytokine expression fold-change was analyzed between each time point using a robust linear regression model and compared between the two treatment groups.Results:Compared to the saline irrigation group,five cytokines were differently expressed (CI =95%) in sinonasal tissue obtained from subjects in the LH irrigation group during the 12-week treatment period.Cytokines IL-6 (P =0.0400),IL-8 (P =0.0398),MCP-1 (P =0.0284),and MIP-1β (P =0.016) were significantly increased in the LH irrigation group compared to the saline irrigation group.IL-13 was significantly increased in the saline irrigation group compared to the LH group (P =0.0086).Conclusion:LH may potentially act to modulate the expression of IL-6,IL-8,IL-13,MCP-1 and MIP-1β in sinonasal tissue.展开更多
文摘Objective:To identify the differences in cytokine expression between sinonasal tissue from patients treated with Leptospermum (Manuka) honey (LH) irrigation versus normal saline irrigation twice-daily for twelve weeks following sinus surgery (FESS).Methods:Forty-six CRS patients were recruited.Sinus tissue biopsies were collected during FESS and then at 5 and 12 weeks postoperatively during the course of treatment.A multiplex cytokine assay quantified the abundance of 17 cytokines in biopsied tissue.Cytokine expression fold-change was analyzed between each time point using a robust linear regression model and compared between the two treatment groups.Results:Compared to the saline irrigation group,five cytokines were differently expressed (CI =95%) in sinonasal tissue obtained from subjects in the LH irrigation group during the 12-week treatment period.Cytokines IL-6 (P =0.0400),IL-8 (P =0.0398),MCP-1 (P =0.0284),and MIP-1β (P =0.016) were significantly increased in the LH irrigation group compared to the saline irrigation group.IL-13 was significantly increased in the saline irrigation group compared to the LH group (P =0.0086).Conclusion:LH may potentially act to modulate the expression of IL-6,IL-8,IL-13,MCP-1 and MIP-1β in sinonasal tissue.
