基于紫外-可见-近红外光谱技术的蔬菜细胞ATP含量无损检测研究

农产品细胞内的ATP含量水平直接反映细胞的活性,在产后贮藏过程中可作为农产品新鲜度和品质的一种评价指标。采用紫外-可见-近红外分光光度计UV-3600获取菠菜叶片样本的光谱数据,并采用常规物理化学方法和萤火虫荧光素酶生物发光技术制备和测定细胞原生质体悬浮液及其细胞原生质体的ATP含量。利用person相关分析确定关键特征波长,并建立基于298nm紫外光和730nm近红外光两个特征波长菠菜叶片光谱反射率的细胞原生质体ATP含量的预测模型。结果分析表明,298nm紫外光和730nm近红外光两个特征波长具有预测细胞原生质体ATP含量的潜力(R2=0.802 9和0.901)。提出的基于光谱技术的蔬菜细胞ATP含量检测方法为准确、快速、无损的蔬菜新鲜度评价提供一种新的技术途径。

A Grading Method for Mangoes on the Basis of Peel Color Measurement Using a Computer Vision System

An objective grading method using a Computer Vision System (CVS) for mangoes is proposed. Red peel was selected using two types of color space values at chroma = 22 and hue angle = 52°. Eighteen out of 25 fully-ripened fruits were graded as “excellent,” determined by the share of red area per fruit being in the range of 80% - 100%. In contrast, all green-mature fruits were graded as “fair,” where the share of red area per fruit was - 0.0542x + 7.83), with a correlation coefficient accuracy of 0.94 and root mean square error of 1.31 mg·kg-1. This result may be effective for the visualization of anthocyanin distribution on mango skin. The threshold for red peel can be in the range of 131 - 186 mg·kg-1. This suggests that the pigment concentration is usable as a universal threshold. This value is unaffected by conditions for image acquisition or color measurement (e.g., light source, sensor, filter, and optical geometry), unlike color space values as hue angle.

3维图像处理系统在稻米品质检测中的应用研究

利用微切片 3维图像处理系统对稻米的品质特性进行了探索性的研究。结果表明 ,借助于 3维可视化技术 ,可以对稻谷的外观品质、营养品质及蒸煮品质等进行更为直观且客观的观察与测定。尤其是在营养成分分布密度的评价、心白米内部组织的分析、蒸煮过程中组织结构变化的观测等方面 ,取得了传统研究方法难以获得的结果。利用该系统对垩白度的定量化测定 ,为食品的定量形态学研究开辟了一条新的研究途径。