The effects of anisodamine and dobutamine on gut mucosal blood flow during gut ischemia/reperfusion

AIM：To detemine if anisodamine is able to augment mucsal perfusion during gut I/R ischemia-reperfusion METHODS:A jejunal sac was formend in Sperague Dawley rat.A Laser Doppler probe and a tonometer were inserted into the sac thich was filled with saline.The superior mesenteric artery was occluded(SMAO)for 60 minutes followed by 90 minutes of reperfusion.At the end of 60 minutes of SMAO.either 0.2mg/kg of anisodmine or dobutamine was injected into the jejunal sac.Lase Doppler mucosal blood flow and regional PCO2(PrCO2) measurements were made .RESULTS:Mucosal blood flow was significantly increased at 30,60 and 90 minutes of reperfusion(R30,R60,R90)when intraluminal anisodamine or dobutamine was present compared to intraluminal saline only(44±3.3%or 48±4.1%vs 37±2.6%at R30,57±5.0%,57±5.0%or56±4.7%vs45±2.7%at R60,64±3.3%or56±4.2%.vs48±3.4%at R90,respectively P<0.05),Blood flow changes were also reflected by lowering of jejunal PrCO2 measurements after imtraluminal anisodamine or dobutamine compared with that of the saline controls(41±3.1mmHg or 44±3.0mmHg vs49±3.7mmHg at R30,38±3.7mmHg or 40±2.1mmHg vs47±3.8mmHg at R60,34±2.1mmHg or 39±3.0mmHg vs46±3.4mmHg at R90,respectively,P<0.05),Most interesting finding was that there were significantly higher mucosal blood flow and lower jejunal PrCO2 in anisodamine group than those in dobutamine group at 90 minutes of reperfusion(64±3.3%vs56±4.2%for blood flow or 34±2.1mmHg vs39±3.0mmHg for PrCOs,respectively,P<0.05).suggesting that anisodamine had a more lasting effect on mucosal perfusion than dobutamine.CONCLUSION.Intraluminal anisodamine and dobutamine can augment mucosal blood flow during gut I/R and alleviate mucosal acidosis.The results provided benifical effects on the treatment of splanchnic hypoperfusion following traumatic or burn shock.