The overuse and misuse of antibiotics have accelerated the selection of antibiotic-resistant bacteria,significantly impacting human,animal,and environmental health.As aquatic environments are vulnerable to antibiotic ...The overuse and misuse of antibiotics have accelerated the selection of antibiotic-resistant bacteria,significantly impacting human,animal,and environmental health.As aquatic environments are vulnerable to antibiotic resistance,suitable management practices should be adopted to tackle this phenomenon.Here we show an effective,nature-based solution for reducing antibiotic resistance from actual wastewater.We utilize a bioreactor that relies on benthic(biofilms)and planktonic microbial communities to treat secondary effluent from a small urban wastewater treatment plant(<10,000 population equivalent).This treated effluent is eventually released into the local aquatic ecosystem.We observe high removal efficiency for genes that provide resistance to commonly used antibiotic families,as well as for mobile genetic elements that could potentially aid in their spread.Importantly,we notice a buildup of sulfonamide(sul1 and sul2)and tetracycline(tet(C),tet(G),and tetR)resistance genes specifically in biofilms.This advancement marks the initial step in considering this bioreactor as a nature-based,cost-effective tertiary treatment option for small UWWTPs facing antibiotic resistance challenges.展开更多
基金Spanish Ministry of Science and Innovation(Project PID2021-127326OB-100)Spanish Ministry of Science and Innovation,the State Investigation Agency and the Regional Development of European Funding(FRENAWASTE Project:PID2022-1385630A-100)+2 种基金L.P has received funding from the Spanish Ministry of Science and Innovation through a Ramón y Cajal contract ‘[RYC 2020-029829-I]’L.B.T has received funding from the European Social Funding Plus (FSE+)Generalitat de Catalunya (grant number 2022_FI_B2 00 027).
文摘The overuse and misuse of antibiotics have accelerated the selection of antibiotic-resistant bacteria,significantly impacting human,animal,and environmental health.As aquatic environments are vulnerable to antibiotic resistance,suitable management practices should be adopted to tackle this phenomenon.Here we show an effective,nature-based solution for reducing antibiotic resistance from actual wastewater.We utilize a bioreactor that relies on benthic(biofilms)and planktonic microbial communities to treat secondary effluent from a small urban wastewater treatment plant(<10,000 population equivalent).This treated effluent is eventually released into the local aquatic ecosystem.We observe high removal efficiency for genes that provide resistance to commonly used antibiotic families,as well as for mobile genetic elements that could potentially aid in their spread.Importantly,we notice a buildup of sulfonamide(sul1 and sul2)and tetracycline(tet(C),tet(G),and tetR)resistance genes specifically in biofilms.This advancement marks the initial step in considering this bioreactor as a nature-based,cost-effective tertiary treatment option for small UWWTPs facing antibiotic resistance challenges.
