Objective:To assess the presence of hepatitis B surface antigen,one of the serologic markers of hepatitis B virus(HBV)infection,in postmortem blood samples from autopsy cases using ELISA,and to compare the results wit...Objective:To assess the presence of hepatitis B surface antigen,one of the serologic markers of hepatitis B virus(HBV)infection,in postmortem blood samples from autopsy cases using ELISA,and to compare the results with those obtained by PCR,which is the gold standard method in assessing HBV infection.Methods:The HBV test results of the blood samples from 880 autopsy cases determined in our laboratory,were retrospectively studied.Results:When compared with the gold standard method PCR,the sensitivity and specificity of postmortem ELISA were 100%and 84.1%,respectively.Conclusions:The increasingly used molecular diagnostic methods,such as PCR,should be used in cases where serological tests remain insufficient.We think that prospective studies on the comparison of ELISA and PCR assessment of postmortem blood samples with larger material should be carried out.展开更多
文摘Objective:To assess the presence of hepatitis B surface antigen,one of the serologic markers of hepatitis B virus(HBV)infection,in postmortem blood samples from autopsy cases using ELISA,and to compare the results with those obtained by PCR,which is the gold standard method in assessing HBV infection.Methods:The HBV test results of the blood samples from 880 autopsy cases determined in our laboratory,were retrospectively studied.Results:When compared with the gold standard method PCR,the sensitivity and specificity of postmortem ELISA were 100%and 84.1%,respectively.Conclusions:The increasingly used molecular diagnostic methods,such as PCR,should be used in cases where serological tests remain insufficient.We think that prospective studies on the comparison of ELISA and PCR assessment of postmortem blood samples with larger material should be carried out.
