Immersion of scaffolds in Simulated Body Fluid(10SBF)is a standardized method for evaluating their bioactivity,simulating in vivo conditions where apatite deposits can be formed on the surface of scaffold,facilitating...Immersion of scaffolds in Simulated Body Fluid(10SBF)is a standardized method for evaluating their bioactivity,simulating in vivo conditions where apatite deposits can be formed on the surface of scaffold,facilitating bone integration and ensuring their suitability for bone implant purposes,ultimately contributing to long-term implant success.The effect of apatite deposition on bioactivity and cell behavior of TiO_(2)scaffolds was studied.Scaffolds were soaked in 10SBF for different durations to form HAP layer on their surface.The results proved the development of a hydroxyapatite film resembling the mineral composition of bone Extracellular Matrix(ECM)on the TiO_(2)scaffolds.The XRD test findings showed the presence of hydroxyapatite layer similar to bone at the depth of 10 nm.A decrease in the specific surface area(18.913 m^(2)g^(−1)),the total pore volume(0.045172 cm^(3)g^(−1)(at p/p0=0.990)),and the mean pore diameter(9.5537 nm),were observed by BET analysis which confirmed the formation of the apatite layer.It was found that titania scaffolds with HAP coating promoted human osteosarcoma bone cell(MG63)cell attachment and growth.It seems that immersing the scaffolds in 10SBF to form HAP coating before utilizing them for bone tissue engineering applications might be a good strategy to promote bioactivity,cell attachment,and implant fixation.展开更多
In this paper,we used Corn Stalk(CS)as a renewable and economical bio template to fabricate willemite scaffolds with the potential application in skull bone repair.CS was used as a sacrificial template to synthesize t...In this paper,we used Corn Stalk(CS)as a renewable and economical bio template to fabricate willemite scaffolds with the potential application in skull bone repair.CS was used as a sacrificial template to synthesize the scaffolds.Willemite scaffolds with the chemical formula of Zn2SiO4 and pore size in the range of 3 to 10µm could be successfully synthesized by soaking CS in the willemite solution for 24 h and sintering at 950°C for 5 h.The porosity of the samples was controlled by the soaking time(between 12 and 48 h)in the willemite solution from 5 to 35%,respectively.The properties of these scaffolds showed a good approximation with cranial bone tissue.In addition,cytotoxicity assays(MTT)were performed on Human Bone Marrow Stromal cells(HBMSc)and A172 human glioblastoma cell lines by direct and indirect culture methods to estimate their toxicity for bone and nerve cells,respectively.Alkaline Phosphatase(ALP)activity and DAPI/Phalloidin cell staining were also performed to investigate the efficiency of the scaffolds for bone tissue engineering applications.The results showed that the scaffolds had good biocompatibility with both HBMSC and A172 cells,noticeable improvement on ALP activity,and great apatite formation ability in Simulated Body Fluid(SBF).All the evidence ascertained that willemite scaffolds made by corn stalks could be a useful candidate for bone tissue engineering applications.展开更多
文摘Immersion of scaffolds in Simulated Body Fluid(10SBF)is a standardized method for evaluating their bioactivity,simulating in vivo conditions where apatite deposits can be formed on the surface of scaffold,facilitating bone integration and ensuring their suitability for bone implant purposes,ultimately contributing to long-term implant success.The effect of apatite deposition on bioactivity and cell behavior of TiO_(2)scaffolds was studied.Scaffolds were soaked in 10SBF for different durations to form HAP layer on their surface.The results proved the development of a hydroxyapatite film resembling the mineral composition of bone Extracellular Matrix(ECM)on the TiO_(2)scaffolds.The XRD test findings showed the presence of hydroxyapatite layer similar to bone at the depth of 10 nm.A decrease in the specific surface area(18.913 m^(2)g^(−1)),the total pore volume(0.045172 cm^(3)g^(−1)(at p/p0=0.990)),and the mean pore diameter(9.5537 nm),were observed by BET analysis which confirmed the formation of the apatite layer.It was found that titania scaffolds with HAP coating promoted human osteosarcoma bone cell(MG63)cell attachment and growth.It seems that immersing the scaffolds in 10SBF to form HAP coating before utilizing them for bone tissue engineering applications might be a good strategy to promote bioactivity,cell attachment,and implant fixation.
文摘In this paper,we used Corn Stalk(CS)as a renewable and economical bio template to fabricate willemite scaffolds with the potential application in skull bone repair.CS was used as a sacrificial template to synthesize the scaffolds.Willemite scaffolds with the chemical formula of Zn2SiO4 and pore size in the range of 3 to 10µm could be successfully synthesized by soaking CS in the willemite solution for 24 h and sintering at 950°C for 5 h.The porosity of the samples was controlled by the soaking time(between 12 and 48 h)in the willemite solution from 5 to 35%,respectively.The properties of these scaffolds showed a good approximation with cranial bone tissue.In addition,cytotoxicity assays(MTT)were performed on Human Bone Marrow Stromal cells(HBMSc)and A172 human glioblastoma cell lines by direct and indirect culture methods to estimate their toxicity for bone and nerve cells,respectively.Alkaline Phosphatase(ALP)activity and DAPI/Phalloidin cell staining were also performed to investigate the efficiency of the scaffolds for bone tissue engineering applications.The results showed that the scaffolds had good biocompatibility with both HBMSC and A172 cells,noticeable improvement on ALP activity,and great apatite formation ability in Simulated Body Fluid(SBF).All the evidence ascertained that willemite scaffolds made by corn stalks could be a useful candidate for bone tissue engineering applications.
