High-density genetic markers are required for genotyping and linkage mapping in identifying genes from crops with complex genomes, such as barley. As the most common variation, single nucleotide polymorphisms(SNPs) ar...High-density genetic markers are required for genotyping and linkage mapping in identifying genes from crops with complex genomes, such as barley. As the most common variation, single nucleotide polymorphisms(SNPs) are suitable for accurate genotyping by using the next-generation sequencing(NGS) technology. Reduced representation libraries(RRLs) of five barley accessions and one mutant were sequenced using NGS technology for SNP discovery. Twenty million short reads were generated and the proportion of repetitive sequences was reduced by more than 56%. A total of 6061 SNPs were identified, and 451 were mapped to the draft sequence of the barley genome with pairing reads. Eleven SNPs were validated using length polymorphic allele-specific PCR markers.展开更多
目的探讨伴有骨髓水肿的老年膝骨关节炎与护骨因子、dickkopf-1(DKK-1)和硬骨素的相关性。方法选取2017年9月-2018年12月四川省骨科医院的老年膝骨关节炎患者100例,分为骨髓水肿组(50例)和非骨髓水肿组(50例),分别收集其基本资料、西安...目的探讨伴有骨髓水肿的老年膝骨关节炎与护骨因子、dickkopf-1(DKK-1)和硬骨素的相关性。方法选取2017年9月-2018年12月四川省骨科医院的老年膝骨关节炎患者100例,分为骨髓水肿组(50例)和非骨髓水肿组(50例),分别收集其基本资料、西安大略和麦克马斯特大学骨关节炎指数(Western Ontario and McMaster University Osteoarthritis Index,WOMAC)评分和视觉模拟评分法得分,检测其血清护骨因子、DKK-1、硬骨素、C反应蛋白、红细胞沉降率,并比较两组之间的差异,探讨其检测指标与骨髓水肿及其临床指标的相关性。结果两组患者的年龄、性别、病程、C反应蛋白和红细胞沉降率差异无统计学意义(P>0.05)。骨髓水肿组患者的WOMAC评分[(76.1±5.4)vs.(67.5±6.6)分]、视觉模拟评分法得分[(8.4±1.1)vs.(5.5±0.9)分]、滑膜炎比例(84.0%vs.52.0%)、护骨因子[(1.3±1.1)vs.(0.6±0.5)μg/L]、DKK-1[(18.4±16.9)vs.(6.9±6.0)μg/L]和硬骨素[(147.3±119.4)vs.(99.7±70.7)pg/mL]均高于非骨髓水肿组(P<0.05)。骨髓水肿组患者的骨髓水肿容积积分和程度积分与血清护骨因子相关性无统计学意义(P>0.05),与血清DKK-1(容积积分rs=0.464,P=0.001;程度积分rs=0.379,P=0.007)、硬骨素(容积积分rs=0.316,P=0.025;程度积分rs=0.461,P=0.003)呈正相关。结论伴有骨髓水肿的老年膝骨关节炎患者局部骨代谢指标护骨因子、DKK-1、硬骨素上调,尤以DKK-1、硬骨素与骨髓水肿的严重程度相关。展开更多
基金supported by the National Natural Science Foundation of China (31000711, 31370032)China Agriculture Research System (CARS-05)the Agricultural Science and Technology Innovation Program
文摘High-density genetic markers are required for genotyping and linkage mapping in identifying genes from crops with complex genomes, such as barley. As the most common variation, single nucleotide polymorphisms(SNPs) are suitable for accurate genotyping by using the next-generation sequencing(NGS) technology. Reduced representation libraries(RRLs) of five barley accessions and one mutant were sequenced using NGS technology for SNP discovery. Twenty million short reads were generated and the proportion of repetitive sequences was reduced by more than 56%. A total of 6061 SNPs were identified, and 451 were mapped to the draft sequence of the barley genome with pairing reads. Eleven SNPs were validated using length polymorphic allele-specific PCR markers.
文摘目的探讨伴有骨髓水肿的老年膝骨关节炎与护骨因子、dickkopf-1(DKK-1)和硬骨素的相关性。方法选取2017年9月-2018年12月四川省骨科医院的老年膝骨关节炎患者100例,分为骨髓水肿组(50例)和非骨髓水肿组(50例),分别收集其基本资料、西安大略和麦克马斯特大学骨关节炎指数(Western Ontario and McMaster University Osteoarthritis Index,WOMAC)评分和视觉模拟评分法得分,检测其血清护骨因子、DKK-1、硬骨素、C反应蛋白、红细胞沉降率,并比较两组之间的差异,探讨其检测指标与骨髓水肿及其临床指标的相关性。结果两组患者的年龄、性别、病程、C反应蛋白和红细胞沉降率差异无统计学意义(P>0.05)。骨髓水肿组患者的WOMAC评分[(76.1±5.4)vs.(67.5±6.6)分]、视觉模拟评分法得分[(8.4±1.1)vs.(5.5±0.9)分]、滑膜炎比例(84.0%vs.52.0%)、护骨因子[(1.3±1.1)vs.(0.6±0.5)μg/L]、DKK-1[(18.4±16.9)vs.(6.9±6.0)μg/L]和硬骨素[(147.3±119.4)vs.(99.7±70.7)pg/mL]均高于非骨髓水肿组(P<0.05)。骨髓水肿组患者的骨髓水肿容积积分和程度积分与血清护骨因子相关性无统计学意义(P>0.05),与血清DKK-1(容积积分rs=0.464,P=0.001;程度积分rs=0.379,P=0.007)、硬骨素(容积积分rs=0.316,P=0.025;程度积分rs=0.461,P=0.003)呈正相关。结论伴有骨髓水肿的老年膝骨关节炎患者局部骨代谢指标护骨因子、DKK-1、硬骨素上调,尤以DKK-1、硬骨素与骨髓水肿的严重程度相关。
