Semantic Consistency and Correctness Verification of Digital Traffic Rules

The consensus of the automotive industry and traffic management authorities is that autonomous vehicles must follow the same traffic laws as human drivers.Using formal or digital methods,natural language traffic rules can be translated into machine language and used by autonomous vehicles.In this paper,a translation flow is designed.Beyond the translation,a deeper examination is required,because the semantics of natural languages are rich and complex,and frequently contain hidden assumptions.The issue of how to ensure that digital rules are accurate and consistent with the original intent of the traffic rules they represent is both significant and unresolved.In response,we propose a method of formal verification that combines equivalence verification with model checking.Reasonable and reassuring digital traffic rules can be obtained by utilizing the proposed traffic rule digitization flow and verification method.In addition,we offer a number of simulation applications that employ digital traffic rules to assess vehicle violations.The experimental findings indicate that our digital rules utilizing metric temporal logic(MTL)can be easily incorporated into simulation platforms and autonomous driving systems(ADS).

Age-related Changes in Humanin Expression in the Ovarian Tissue of Rat

Objective This study examined humanin expression in rat ovarian tissue,its cellular localization,and its correlation with rat age under physiological conditions.Methods A total of 40 Sprague-Dawley rats of various ages(2,12,30,and 60 days old and 1 year old)were grouped by age.Immunofluorescence and immunohistochemical techniques were used to observe humanin expression and cellular location in the ovarian tissues of rats from each age group.In addition,Western blotting and Real-time quantitative reverse transcription PCR(qRT-PCR)techniques were used to measure humanin expression level in the ovarian tissues of rats from each age group.Results The immunofluorescence and immunohistochemical results confirmed that humanin was expressed in rat ovarian tissues.In addition,cellular localization analysis showed that humanin was expressed in the cytoplasm of oocytes,interstitial cells,granulosa cells and theca cells in all levels of follicles after the primary follicles,and in the corpus luteum.The qRT-PCR results revealed that the level of humanin expression in the ovarian tissues of 12-day-old rats was non-significantly higher than that in the ovarian tissues of 2-day-old rats(P>0.05),whereas the levels of humanin expression in the ovarian tissues of 30-day-old,60-day-old,and 1-year-old rats were significantly lower than that in the ovarian tissues of 2-day-old rats(P<0.05).The Western blotting results demonstrated that the levels of humanin protein expression in the ovarian tissues of 60-day-old and 1-year-old rats were significantly lower than those of 2-day-old rats(P<0.01),whereas there was no significant difference in the level of humanin protein expression between the ovarian tissues of 12-day-old and 30-day-old rats.Conclusion This study confirmed that humanin is expressed in the cytoplasm of various cells in rat ovarian tissues.Moreover,the level of humanin expression was highest in the ovarian tissues of 12-day-old rats,and it subsequently decreased with age.The changes in the expression of humanin in the ovary of rats at different ages will lay the foundation for the role of humanin in ovarian aging.The effect of humanin on ovarian function is worthy of further study in the future.

环磷酰胺联合人免疫球蛋白治疗老年SLE的有效性及安全性分析

目的探讨环磷酰胺联合人免疫球蛋白治疗老年系统性红斑狼疮(SLE)的有效性及安全性。方法选取2012年10月-2014年2月该院收治的74例SLE患者。采用随机数字表法,将患者分为研究组和对照组。每组37例。对照组患者给予环磷酰胺治疗。研究组患者给予环磷酰胺联合人免疫球蛋白治疗。对两组患者临床疗效、治疗前后SLE疾病活动指数(SLEDAI)、24 h尿蛋白及不良反应发生情况进行比较。结果与对照组比较,研究组患者临床治疗显效率和总有效率提高,而无效率降低;治疗后SLEDAI评分和24 h尿蛋白含量降低,不良反应高血压和高血脂的发生率下降(P<0.05)。结论环磷酰胺联合人免疫球蛋白不仅能够提高老年SLE患者的临床疗效,还能进一步降低不良反应的发生率,进而提高老年患者治疗的安全性。

Study on the correlation of MLCK and FAP expression with uterine fibroid cell proliferation and invasion

Objective:To study the correlation of myosin light chain kinase (MLCK) and fibroblast activation protein (FAP) expression with uterine fibroid cell proliferation and invasion. Methods:Uterine fibroids samples and normal uterine muscle samples next to fibroids that were surgically removed in Wuhan Red Cross Hospital between May 2014 and January 2017 were chosen, fluorescence quantitative PCR kits were used to deterct MLCK and FAP mRNA expression, and enzyme-linked immunosorbent assay kits were used to determine proliferation and invasion gene protein expression.Results: MLCK and FAP mRNA expression in uterine fibroids samples were significantly higher than those in normal uterine muscle samples, and Survivin, Livin, Bcl-2, Snail, N-cadherin and MMP2 protein expression were significantly higher than those in normal uterine muscle samples;Survivin, Livin, Bcl-2, Snail, N-cadherin and MMP2 protein expression in uterine fibroids samples with high MLCK and FAP expression were significantly higher than those in uterine fibroids samples with low MLCK and FAP expression.Conclusion: Highly expressed MLCK and FAP in uterine fibroids can promote the proliferation and invasion of uterine fibroids.

Effect of folic acid and vitamin supplement on endothelial injury and placental blood perfusion in patients with hypertensive disorder complicating pregnancy

Objective: To study the effect of folic acid and vitamin supplement on endothelial injury and placental blood perfusion in patients with hypertensive disorder complicating pregnancy. Methods: A total of 62 patients who were diagnosed with preeclampsia in Wuhan Red Cross hospital between March 2015 and February 2017 were selected as the research subjects and randomly divided into two groups, the observation group received folic acid, vitamin and conventional symptomatic treatment, and control group accepted conventional symptomatic treatment. Serum contents of endothelial injury markers and oxidative stress indexes were detected before treatment and 2 weeks after treatment;the expression of apoptosis genes in the placenta were detected after delivery. Results: 2 weeks after treatment, serum ET-1, tTG, sFlt-1, sEng, MPO, IMA, MDA and AOPP levels of both groups of patients were significantly lower than those before treatment, and serum ET-1, tTG, sFlt-1, sEng, MPO, IMA, MDA and AOPP levels of observation group were significantly lower than those of control group;after delivery, Fas, Bax and Caspase-3 mRNA expression levels in placenta tissue of observation group were significantly lower than those of control group while Bcl-2, Survivin and Livin mRNA expression levels were significantly higher than those of control group. Conclusion:Folic acid and vitamin supplement can significantly improve the endothelial injury and placental blood perfusion in patients with hypertensive disorder complicating pregnancy.

Secure, Efficient and Searchable File System on Distributed Clouds

Many enterprises and personals are inclining to outsource their data to public clouds, but security and privacy are two critical problems cannot be ignored. The door of cloud provider may be broken, and the data may also be dug into by providers to find valuable information. In this paper, a secure and efficient storage file (SES FS) system is proposed to distribute files in several clouds and allows users to search the files securely and efficiently. In the proposed system, keywords were transformed into integers and secretly shared in a defined finite field, then the shares were mapped to random numbers in specified random domain in each cloud. Files were encrypted with distinct secret key and scattered within different clouds. Information about keyword/file was secretly shared among cloud providers. Legal users can search in the clouds to find correct encrypted files and reconstruct corresponding secret key. No adversary can find or detect the real file information even they can collude all the servers. Manipulation on shares by one or more clouds can be detected with high probability. The system can also detect malicious servers through introduced virtual points. One interesting property for the scheme is that new keywords can be added easily, which is difficult and usually not efficient for many searchable symmetric encryption systems. Detailed experimental result shows, with tolerable uploading delay, the scheme exhibits excellent performance on data retrieving aspect.