期刊文献+
共找到4篇文章
< 1 >
每页显示 20 50 100
美国红枫转录组SSR序列分析 被引量:7
1
作者 郭聪 陈燕 +4 位作者 王莹 谈峰 柯裴蓓 沙文锋 李玉娟 《中南林业科技大学学报》 CAS CSCD 北大核心 2021年第7期132-141,共10页
【目的】旨在全面了解叶色多彩明亮、抗逆性强的美国红枫SSR位点信息及序列特征,为开发新的功能基因相关且多态性良好的SSR标记提供依据。【方法】以美国红枫不同叶色的叶片为试验材料,经转录组测序后,利用MISA软件对美国红枫1 kb以上的... 【目的】旨在全面了解叶色多彩明亮、抗逆性强的美国红枫SSR位点信息及序列特征,为开发新的功能基因相关且多态性良好的SSR标记提供依据。【方法】以美国红枫不同叶色的叶片为试验材料,经转录组测序后,利用MISA软件对美国红枫1 kb以上的Unigene进行SSR位点搜索;通过Excel软件进行SSR分布和序列特征分析并用Prime 3软件设计引物。【结果】从美国红枫转录组测序数据中共获得Unigene 78 571条,其中长度≥1 kb的Unigene共23 278条。对长度≥1 kb的Unigene进行SSR位点搜索和分析,共获得12 974个SSR位点,出现频率为55.75%,平均约3.7 kb出现1个SSR位点;美国红枫转录组SSR重复碱基类型共有7种,其中单碱基为主要重复类型,占总SSR的54.12%,其次为双碱基和三碱基重复类型,分别占总SSR的20.64%和16.19%,其他的碱基重复类型较低,仅占总SSR的9.05%。A/T、AT/TA与GAA/TTC分别为单碱基到三碱基的优势重复基元,分别占总SSR的53.95%、8.00%与1.65%,而四碱基的重复基元均较低,在0.05%以下;单碱基到六碱基各基元重复次数集中在5~22次之间,序列长度变化在10~48 bp之间,平均长度为18.43 bp;筛选出10751个SSR符合引物设计要求,共设计出32253对引物,占总SSR的82.87%。【结论】根据转录组测序结果,美国红枫转录组中SSR位点分布频率高,基序类型丰富,具有较高的多态性,在美国红枫遗传多样性和分子标记辅助育种中具有较大的应用潜力。 展开更多
关键词 美国红枫 SSR 转录组 序列分析
下载PDF
Application of Probiotics in Pig Industry as a Substitute of Antibiotics 被引量:2
2
作者 sha wenfeng Zhu Juan +2 位作者 Gu Yongjian Dai Hui Qiu Liangwei 《Animal Husbandry and Feed Science》 CAS 2015年第1期15-17,38,共4页
Antibiotics plays a positive role in the development of livestock breeding and feed industry,but has also disadvantages impossible to overcome,so it is an inevitable trend of prohibiting application of antibiotics in ... Antibiotics plays a positive role in the development of livestock breeding and feed industry,but has also disadvantages impossible to overcome,so it is an inevitable trend of prohibiting application of antibiotics in feed.This paper reviewed concept and action mechanism of the antibiotics substitute—probiotics,and its application in piglets,growing and fattening pigs and sows. 展开更多
关键词 Antibiotics Probiotics Pig industry Research application
下载PDF
Research Advance of Goat Fattening Technology
3
作者 sha wenfeng 《Animal Husbandry and Feed Science》 CAS 2017年第3期165-167,171,共4页
Effects of breeding mode, feeding mode, feed and nutritional level on fattening performance of goat are summarized. Moreover, several suggestions are put forward for research direction of goat fattening technology in ... Effects of breeding mode, feeding mode, feed and nutritional level on fattening performance of goat are summarized. Moreover, several suggestions are put forward for research direction of goat fattening technology in the future. 展开更多
关键词 GOAT Fattening technology Research advance
下载PDF
Effects of Different Antibiotics Substitutes on Production Performance of Fattening Pigs
4
作者 sha wenfeng Zhu Juan +2 位作者 Gu Yongjian Zhang Ruixiang Shen Jun 《Animal Husbandry and Feed Science》 CAS 2016年第5期275-277,共3页
[ Objective ] The aim was to discuss effects of different antibiotics substitutes on production performance of fattening pigs, and to lay a foundation for development and application of antibiotics-free pig feed. [ Me... [ Objective ] The aim was to discuss effects of different antibiotics substitutes on production performance of fattening pigs, and to lay a foundation for development and application of antibiotics-free pig feed. [ Method ] By adding 100 mg/kg compound probiotics, 100 mg/kg compound enzyme preparation, 500 and 1 000 mg/kg eu-commin in the basal diet, binary hybrid pigs weighing about 15 kg were reared for 102 d, and the feed added with 300 mg/kg colistin sul- fate was used as control. [ Result] Using compound prebiotics and compound enzyme preparation to substitute antibiotics had no adverse effects on daily weight gain, daily feed intake and feed gain ratio of 15 -90 kg fattening pigs; using eu-commin to substitute antibiotics had no adverse effect on daily weight gain, daily feed intake and feed gain ratio of 15 - 30 kg fattening pigs, but had significant influence on daily weight gain of 30 - 90 kg fattening pigs ( P 〈 0.05 ). [ Conclusion] It is feasible to develop antibiotics-free pig feed by using new type of green environmental-friendly additive instead of antibiotics. 展开更多
关键词 Antibiotics substitutes Fattening pig Production performance
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部