期刊文献+
共找到5篇文章
< 1 >
每页显示 20 50 100
红芪多糖“脱蛋白-大孔吸附树脂”的纯化富集工艺考察
1
作者 赵沙沙 何海 +3 位作者 张小荣 郭玫 崔治家 邵晶 《药物流行病学杂志》 CAS 2023年第6期679-688,共10页
目的研究红芪多糖(HPS)标准提取物,筛选优化其“脱蛋白-大孔吸附树脂纯化富集”的工艺方法与工艺参数。方法以蛋白脱除率和多糖损失率为指标,比较4种不同方法的脱蛋白效果,并对筛选出的方法进行工艺参数优化;以吸附量和吸附率为指标,考... 目的研究红芪多糖(HPS)标准提取物,筛选优化其“脱蛋白-大孔吸附树脂纯化富集”的工艺方法与工艺参数。方法以蛋白脱除率和多糖损失率为指标,比较4种不同方法的脱蛋白效果,并对筛选出的方法进行工艺参数优化;以吸附量和吸附率为指标,考察4种型号大孔吸附树脂(AB-8、D-101、NKA-9、X-5)对HPS的纯化富集效果,并进行相关工艺参数优化;采用苯酚硫酸法和考马斯亮兰法分别测定多糖和蛋白质含量;采用紫外分光光度(UV)和红外光谱(IR)法比较纯化前后多糖的基本特征。结∶果筛选出的脱蛋白方法为Sevag法,优选的树脂为AB-8大孔吸附树脂。通过工艺参数优选,确定的纯化工艺为:10 mg·mL^(-1)粗多糖水溶液,以1/2倍体积氯仿^(-1),∶v/v)混合试剂进行脱蛋白操作2次;除去残留有机溶剂后,以质量浓度为1.5 mg·mL^(-1)的水溶液,上预处理好的AB-8大孔吸附树脂柱[树脂量(g)与上样体10]∶,以2.0 mL·min^(-1)流速循环上样2次,动态吸附后再静态吸附6~8 h,后以30%乙醇1.0 mL·min^(-1)进行洗脱,至洗脱液无明显碱性酒石酸铜反应,收集洗脱液浓缩至与原药1冲洗沉淀,即得HPS。结论验证试验表明,经本工艺纯化富集后,所得HPS样品纯度提高了1.27倍;UV在260,280 nm处未见蛋白质吸收峰;IR表现出更突出的多糖结构特征吸收峰,说明纯化过程有效且未对HPS主要结构产生影响。优选出的HPS脱蛋白和大孔树脂纯化工艺稳定可行,重复性较好,使多糖得到较好的纯化富集,为HPS标准提取物的形成以及其能成为制剂科学化原料药提供了研究基础。 展开更多
关键词 红芪多糖 脱蛋白 大孔吸附树脂 纯化工艺
下载PDF
Effect of fast-track surgery in gynecological laparoscopy:a randomized controlled trial 被引量:3
2
作者 Xue-Ping Zhu sha-sha zhao Jie-Dan Qin 《Frontiers of Nursing》 CAS 2020年第3期235-238,共4页
Objective:To evaluate the value of fast-track surgery(FTS)in gynecological laparoscopy during the perioperative period.Methods:The clinical data of 234 patients who underwent gynecological laparoscopy from January to ... Objective:To evaluate the value of fast-track surgery(FTS)in gynecological laparoscopy during the perioperative period.Methods:The clinical data of 234 patients who underwent gynecological laparoscopy from January to December in 2018 were collected.Of note,117 patients received a routine nursing method(control group),and 117 patients received the FTS nursing method(observation group).Results:Compared with the control group,FTS group had a quicker postoperative bowel function recovery,earlier to get out of bed,and a lower anxiety level(P<0.05);The incidence of postoperative nausea and vomiting of two groups had no statistical difference.Conclusions:The application of FTS in gynecological laparoscopy is safe,effective,and worth promoting. 展开更多
关键词 fast-track surgery perioperative period ANXIETY LAPAROSCOPY GYNECOLOGICAL POSTOPERATIVE
下载PDF
Mechanical and Microstructural Characteristics of Superplastic Al-4. 42Mg Aluminum Alloy
3
作者 sha-sha zhao Rehan Qayyume +3 位作者 Hao-Yan Diao Chao-Li Ma Xiao-Wei Wu Yong Wang 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2013年第5期96-100,共5页
The superplastic response of commercial 5083 alloy( Al-4. 42Mg) under uniaxial tension at strain rates ranging from 5 × 10- 5to 10- 2s- 1in the temperature interval 400- 550 ℃ was systematically studied in this ... The superplastic response of commercial 5083 alloy( Al-4. 42Mg) under uniaxial tension at strain rates ranging from 5 × 10- 5to 10- 2s- 1in the temperature interval 400- 550 ℃ was systematically studied in this paper. The tension test was conducted on samples of rolling direction. The maximum elongation-to-failure of 486% was found at 500 ℃ and strain rate of 10- 4s- 1. To identify the main characteristics of superplastic deformation and to determine the mechanism of superplastic deformation of the alloy,the microstructure and fracture of the alloy were analyzed as a function of strain,strain rate and temperature using optical microscopy( OM) and scanning electron microscopy( SEM),the apparent strain rate sensitivity exponent m a and the apparent activation energy Q a were also studied. Based on the fracture analysis and the calculated data of m a and Q a,it is suggested that the dominant deformation mechanism in the present alloy is grain boundary sliding( GBS) during the best deformation condition. 展开更多
关键词 5083 alloy SUPERPLASTICITY CAVITY FRACTURE MICROSTRUCTURE
下载PDF
Hot Deformation Behavior of Aluminum Alloy 5083 for Automotive and Aviation Applications
4
作者 Rehan Qayyume sha-sha zhao +3 位作者 Hao-Yan Diao Chao-Li Ma Xiao-Wei Wu Yong Wang 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2013年第5期87-95,共9页
This article concentrates on the investigation of hot deformation behavior of conventionally rolled commercial grade AA5083 alloy( Al-4. 5Mg),for automotive and aviation applications. The superplastic response of the ... This article concentrates on the investigation of hot deformation behavior of conventionally rolled commercial grade AA5083 alloy( Al-4. 5Mg),for automotive and aviation applications. The superplastic response of the alloy was investigated at high strain rates( ≥10- 3s- 1),and a temperature range of 400 ℃ to 550 ℃. An elongation to failure of 201% was achieved at low temperature( 425 ℃) and high strain rate( 10- 2s- 1),which indicates sufficient ductility under hot deformation for manufacturing of extremely complex shapes using superplastic forming technology. Furthermore,the alloy exhibited a maximum elongation of about 470% at strain rate of 10- 3s- 1and a temperature of 525 ℃. The deformation and failure mechanisms at both the critical conditions were studied as a function of strain rate and temperature. The contributions of strain-rate sensitivity and strain hardening were analyzed in relation to the observed tensile ductilities. Deformation mechanism of the alloy was also investigated with reference to Strain rate sensitivity index( m) and Activation energy( Q) for the given test condition. Empirical calculations reveal that dominant deformation mechanism responsible for hot deformation of the alloy is grain boundary sliding( GBS),which is further supported by deformed surface examination using scanning electron microscopy( SEM). Fracture surfaces of the samples deformed to failure,at relatively higher and lower strain rates,was examined to investigate the micromechanisms governing failure. Phenomenon of cavity nucleation,growth and coalescence was observed to be the failure mechanism in the investigated alloy. 展开更多
关键词 Al-Mg alloys SUPERPLASTICITY hot deformation microstructure evolution CAVITATION
下载PDF
支原体污染通过耗竭L-精氨酸降低HEK-293细胞中质粒DNA转染效率(英文) 被引量:1
5
作者 Zi-fei YIN Ya-ni ZHANG +3 位作者 Shu-fang LIANG sha-sha zhao Juan DU Bin-bin CHENG 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2019年第12期1021-1026,共6页
目的:明确支原体污染对HEK-293细胞质粒DNA转染效率的影响,并从支原体对细胞精氨酸代谢的角度探究其机制。创新点:支原体是细胞培养中的常见污染源。HEK-293是目前常用的生产蛋白、包装病毒的常用细胞系。然而,目前支原体污染对于质粒DN... 目的:明确支原体污染对HEK-293细胞质粒DNA转染效率的影响,并从支原体对细胞精氨酸代谢的角度探究其机制。创新点:支原体是细胞培养中的常见污染源。HEK-293是目前常用的生产蛋白、包装病毒的常用细胞系。然而,目前支原体污染对于质粒DNA转染效率的影响未见报道。本研究首次报道支原体污染对HEK-293细胞质粒DNA转染效率的影响,并揭示其机理。方法:采用4’,6-二脒基-2-苯基吲哚(DAPI)和聚合酶链反应(PCR)方法鉴定HEK-293细胞中的支原体污染情况以及支原体抗生素Plasmocin对支原体污染的清除效果。通过聚乙烯亚胺(PEI)方法对支原体污染的HEK-293细胞及支原体清除后的HEK-293细胞转染质粒,比较转染效率差异。通过高效液相色谱法(HPLC)分析支原体污染的和支原体清除后的HEK-293细胞的细胞裂解产物和细胞上清中的L-精氨酸、瓜氨酸含量变化。在支原体污染的HEK-293细胞中补充L-精氨酸,观察质粒转染效率的改变情况。结论:支原体污染能大大降低HEK-293细胞中质粒DNA的转染效率,且其原因与支原体能耗竭细胞中的L-精氨酸有关。 展开更多
关键词 支原体 质粒 转染效率 精氨酸 HEK-293细胞
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部