Proximate composition,mineral contents,phytochemical constituents,antimicrobial activities and Fourier transforms infrared spectroscopy analysis of bark,stem and seed of Hippophae rhamnoides Linn

Objective:To compare the proximate composition,mineral contents,antimicrobial,phytochemical and Fourier transforms infrared(FTIR)spectroscopy analysis of bark,stem and seed of Hippophae rhamnoides.Methods:Proximate composition was determined according to the described methods.Mineral analysis was carried out by atomic absorption spectroscopy and flame photometer.Antimicrobial activities were evaluated according to the agar well diffusion method.Phytochemical qualitative analysis was carried out according to the described methods and functional groups were determined by FTIR Prestige-21 Shimadzu Japan.Results:The proximate analysis showed high content of protein and fiber in stem and bark.High content of Na(900 mg/L)and K(670 mg/L)was found in bark powder,while in seed,high contents of Ca(800 mg/L),Mg(725 mg/L),Fe(250 mg/L)Zn(90 mg/L)and Mn(65 mg/L)were found compared to stem and bark.Phenols,flavonoids and tannins showed high contents in stem and bark of all extracts.The bark aqueous extract showed high zone of inhibition against Staphylococcus aureus(21 mm)and Escherichia coli(20 mm),while methanol extract of stem showed high zone of inhibition(14 mm and 13 mm)against Enterococcus faecalis and Escherichia coli respectively.The aqueous extract of bark documented high zone of inhibition against Aspergillus niger(21 mm)and Aspergillus parasiticus(20 mm).FTIR spectra revealed the presence of OH,C-O and C=O functional groups.Conclusions:The study concludes that bark,stem and seed extracts will be useful guideline for the new syntheses of feed,food supplements and herb drugs with various combination,which can be used for the treatment of many diseases at global level especially in tropical regions as well as the male nutrition problems in these areas.