Cotton leaf curl virus is the first example of acotton-infecting virus where infectious clones areavailable.Plant viruses are valuable tools inunderstanding plant biology as they can beengineered for expression of for...Cotton leaf curl virus is the first example of acotton-infecting virus where infectious clones areavailable.Plant viruses are valuable tools inunderstanding plant biology as they can beengineered for expression of foreign genes orsilencing of genes homologous to cloned genes.展开更多
Cotton leaf curl virus is the first example of a cotton-infecting virus where infectious clones are available. Plant viruses are valuable tools in understanding plant biology as they can be engineered for expressi... Cotton leaf curl virus is the first example of a cotton-infecting virus where infectious clones are available. Plant viruses are valuable tools in understanding plant biology as they can be engineered for expression of foreign genes or silencing of genes homologous to cloned genes.
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RNA interference （RNAi） of vital insect genes is a potential tool for targeted pest control. However, selection of the right target genes is a challenge because the RNAi efficacy is known to vary among insect specie...RNA interference （RNAi） of vital insect genes is a potential tool for targeted pest control. However, selection of the right target genes is a challenge because the RNAi efficacy is known to vary among insect species. Cotton mealybug, Phenacoccus solenopsis, is a phloem-feeding economically important crop pest. We evaluated the RNAi of 2 vital genes, Bursicon （PsBur） and V-ATPase （Ps V-ATPase） as potential targets in P. solenopsis for its control. PCR fragments of PsBur and PsV-ATPase were amplified using cDNA synthesized from the total RNA. The PCR amplicons were cloned into Potato virus X （PVX） to develop recombinant PVX for the inoculation ofNicotiana tabacum plants for bioassays with healthy P. solenopsis. Reverse-transcription-polymerase chain reaction （RT- PCR） was used to validate the expression oftransgenes in the recombinant-PVX-inoculated plants （treated）, and suppression of the target genes in the mealybugs exposed to them. The RT-PCR confirmed the expression of transgenes in the treated plants. Mealybug individuals on treated plants either died or showed physical deformities. Further, the population of mealybug was significantly reduced by feeding on N. tabacum expressing RNAi triggers against PsBur and Ps V-A TPase. The results conclude that RNAi is activated in P. solenopsis by feeding on N. tabacum expressing RNAi triggering elements of PsBur and Ps V-ATPase genes through recombinant PVX vector. Further, V-ATPase and Bursicon genes are potential targets for RNAi-mediated control ofP. solenopsis.展开更多
Wheat production is often affected by different types of rust diseases,in which stripe rust is triggered by a fungal pathogen,Puccinia striiformis f.sp.tritici(Pst),and remains the biggest challenge to plant breeders ...Wheat production is often affected by different types of rust diseases,in which stripe rust is triggered by a fungal pathogen,Puccinia striiformis f.sp.tritici(Pst),and remains the biggest challenge to plant breeders and the farming community.Nevertheless,pathogens exploit its effector proteins for altering the susceptibility(S)genes in the host plant,rendering host defense inactivated.Thus,altering the S genes by employing precise genome editing tools provides a great opportunity to develop broad-spectrum and durable,resistant crops.Recently,Wang et al.,2022 have demonstrated the TaPsIPK1 as an ideal S gene target to confer stripe rust resistance in wheat,providing an opportunity to deliver broad-spectrum,rust-resistant crop varieties.展开更多
Soil-borne pathogens,e.g.,Plasmodiophora brassicae,causes devastating clubroot disease,and existing identified resistance(R)genes has been broken down with the co-evolution of pathogen,threatening the future of crucif...Soil-borne pathogens,e.g.,Plasmodiophora brassicae,causes devastating clubroot disease,and existing identified resistance(R)genes has been broken down with the co-evolution of pathogen,threatening the future of cruciferous crops.Thus,there is a dire need to identify new R genes for developing clubroot-resistant crops.Recently,Wang et al.,2023 have reported a new R gene,WeiTsing,that confers complete resistance to Plasmodiophora brassicae,providing an opportunity to deliver broad-spectrum,durable clubroot-resistant crop varieties.展开更多
文摘Cotton leaf curl virus is the first example of acotton-infecting virus where infectious clones areavailable.Plant viruses are valuable tools inunderstanding plant biology as they can beengineered for expression of foreign genes orsilencing of genes homologous to cloned genes.
文摘 Cotton leaf curl virus is the first example of a cotton-infecting virus where infectious clones are available. Plant viruses are valuable tools in understanding plant biology as they can be engineered for expression of foreign genes or silencing of genes homologous to cloned genes.
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文摘RNA interference （RNAi） of vital insect genes is a potential tool for targeted pest control. However, selection of the right target genes is a challenge because the RNAi efficacy is known to vary among insect species. Cotton mealybug, Phenacoccus solenopsis, is a phloem-feeding economically important crop pest. We evaluated the RNAi of 2 vital genes, Bursicon （PsBur） and V-ATPase （Ps V-ATPase） as potential targets in P. solenopsis for its control. PCR fragments of PsBur and PsV-ATPase were amplified using cDNA synthesized from the total RNA. The PCR amplicons were cloned into Potato virus X （PVX） to develop recombinant PVX for the inoculation ofNicotiana tabacum plants for bioassays with healthy P. solenopsis. Reverse-transcription-polymerase chain reaction （RT- PCR） was used to validate the expression oftransgenes in the recombinant-PVX-inoculated plants （treated）, and suppression of the target genes in the mealybugs exposed to them. The RT-PCR confirmed the expression of transgenes in the treated plants. Mealybug individuals on treated plants either died or showed physical deformities. Further, the population of mealybug was significantly reduced by feeding on N. tabacum expressing RNAi triggers against PsBur and Ps V-A TPase. The results conclude that RNAi is activated in P. solenopsis by feeding on N. tabacum expressing RNAi triggering elements of PsBur and Ps V-ATPase genes through recombinant PVX vector. Further, V-ATPase and Bursicon genes are potential targets for RNAi-mediated control ofP. solenopsis.
文摘Wheat production is often affected by different types of rust diseases,in which stripe rust is triggered by a fungal pathogen,Puccinia striiformis f.sp.tritici(Pst),and remains the biggest challenge to plant breeders and the farming community.Nevertheless,pathogens exploit its effector proteins for altering the susceptibility(S)genes in the host plant,rendering host defense inactivated.Thus,altering the S genes by employing precise genome editing tools provides a great opportunity to develop broad-spectrum and durable,resistant crops.Recently,Wang et al.,2022 have demonstrated the TaPsIPK1 as an ideal S gene target to confer stripe rust resistance in wheat,providing an opportunity to deliver broad-spectrum,rust-resistant crop varieties.
文摘Soil-borne pathogens,e.g.,Plasmodiophora brassicae,causes devastating clubroot disease,and existing identified resistance(R)genes has been broken down with the co-evolution of pathogen,threatening the future of cruciferous crops.Thus,there is a dire need to identify new R genes for developing clubroot-resistant crops.Recently,Wang et al.,2023 have reported a new R gene,WeiTsing,that confers complete resistance to Plasmodiophora brassicae,providing an opportunity to deliver broad-spectrum,durable clubroot-resistant crop varieties.
